9780805376487

Microbiology : A Laboratory Manual

by ;
  • ISBN13:

    9780805376487

  • ISBN10:

    0805376488

  • Edition: 6th
  • Format: Paperback
  • Copyright: 2001-07-01
  • Publisher: Pearson College Div
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Supplemental Materials

What is included with this book?

Summary

This versatile laboratory manual can be used with any undergraduate microbiology text and course. Known for its brief laboratory activities, minimal equipment requirements, and competitive price, the manual includes a variety of experiments selected to assist in the teaching of basic principles and techniques. Each of the 77 experiments includes an overview, a purpose, an in-depth discussion of the principle involved, easy-to-follow procedures, and lab reports with review and critical thinking questions. Comprehensive introductory material and laboratory safety instructions are provided. For college instructors and students.

Table of Contents

Preface ix
Laboratory Safety: General Rules and Regulations xiii
Laboratory Protocol xv
PART 1 Basic Laboratory Techniques for Isolation, Cultivation, and Cultural Characterization of Microorganisms 1(26)
Introduction
1(6)
Culture Transfer Techniques
7(6)
Techniques for Isolation of Pure Cultures
13(8)
Isolation of Discrete Colonies from a Mixed Culture
13(2)
Isolation of Pure Cultures from a Spread-Plate or Streak-Plate Preparation
15(6)
Cultural Characteristics of Microorganisms
21(6)
PART 2 Microscopy 27(18)
Introduction
27(2)
Microscopic Examination of Stained Cell Preparations
29(8)
Microscopic Examination of Living Bacterial Preparations
37(4)
The Microscopic Measurement of Microorganisms
41(4)
PART 3 Bacterial Staining 45(38)
Introduction
45(4)
Preparation of Bacterial Smears
49(6)
Simple Staining
55(4)
Negative Staining
59(4)
Gram Stain
63(6)
Acid-Fast Stain (Ziehl-Neelsen Method)
69(6)
Differential Staining for Visualization of Bacterial Cell Structure
75(8)
Spore Stain (Schaeffer-Fulton Method)
75(1)
Capsule Stain
76(7)
PART 4 Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Populations 83(50)
Introduction
83(4)
Nutritional Requirements: Media for the Routine Cultivation of Bacteria
87(6)
Use of Differential and Selective Media
93(6)
Physical Factors: Temperature
99(4)
Physical Factors: pH of the Extracellular Environment
103(4)
Physical Factors: Atmospheric Oxygen Requirements
107(6)
Techniques for the Cultivation of Anaerobic Microorganisms
113(6)
Serial Dilution-Agar Plate Procedure to Quantitate Viable Cells
119(6)
The Bacterial Growth Curve
125(8)
PART 5 Biochemical Activities of Microorganisms 133(66)
Introduction
133(4)
Extracellular Enzymatic Activities of Microorganisms
137(6)
Carbohydrate Fermentation
143(6)
Triple Sugar-Iron Agar Test
149(4)
IMViC Test
153(10)
Indole Production Test
153(1)
Methyl Red Test
154(1)
Voges-Proskauer Test
155(1)
Citrate Utilization Test
156(7)
Hydrogen Sulfide Test
163(4)
Urease Test
167(4)
Litmus Milk Reactions
171(6)
Nitrate Reduction Test
177(4)
Catalase Test
181(4)
Oxidase Test
185(4)
Utilization of Amino Acids
189(6)
Decarboxylase Test
189(1)
Phenylalanine Deaminase Test
190(5)
Genus Identification of Unknown Bacterial Cultures
195(4)
PART 6 The Protozoa 199(12)
Introduction
199(2)
Free-Living Protozoa
201(4)
Parasitic Protozoa
205(6)
PART 7 The Fungi 211(16)
Introduction
211(2)
Cultivation and Morphology of Molds
213(4)
Yeast Morphology
217(4)
Identification of Unknown Fungi
221(6)
PART 8 The Viruses 227(16)
Introduction
227(4)
Cultivation and Enumeration of Bacteriophages
231(6)
Isolation of Coliphages from Raw Sewage
237(6)
PART 9 Physical and Chemical Agents for the Control of Microbial Growth 243(42)
Introduction
243(4)
Physical Agents of Control: Moist Heat
247(8)
Physical Agents of Control: Environmental Osmotic Pressure
255(4)
Physical Agents of Control: Electromagnetic Radiations
259(4)
Chemical Agents of Control: Chemotherapeutic Agents
263(10)
The Kirby-Bauer Antibiotic Susceptibility Test Procedure
264(2)
Synergistic Effect of Drug Combinations
266(7)
Determination of Penicillin Activity in the Presence and Absence of Penicillinase
273(4)
Chemical Agents of Control: Disinfectants and Antiseptics
277(8)
Phenol Coefficient
280(1)
Agar Plate-Sensitivity Method
281(4)
PART 10 Microbiology of Food 285(18)
Introduction
285(2)
Methylene Blue Reductase Test
287(4)
Microbiological Analysis of Food Products: Bacterial Count
291(4)
Wine Production
295(4)
Sauerkraut Production
299(4)
PART 11 Microbiology of Water 303(16)
Introduction
303(2)
Standard Qualitative Analysis of Water
305(8)
Presumptive Test: Determination of the Most Probable Number of Coliform Bacteria
305(3)
Confirmed Test
308(1)
Completed Test
308(5)
Quantitative Analysis of Water: Membrane Filter Method
313(6)
PART 12 Microbiology of Soil 319(32)
Introduction
319(2)
Nitrogen Cycle
321(12)
Ammonification
321(2)
Nitrification
323(1)
Denitrification
324(1)
Nitrogen Fixation
324(9)
Microbial Populations in Soil: Enumeration
333(6)
Isolation of Antibiotic-Producing Microorganisms and Determination of Antimicrobial Spectrum of Isolates
339(6)
Isolation of Antibiotic-Producing Microorganisms
340(1)
Determination of Antimicrobial Spectrum of Isolates
341(4)
Isolation of Pseudomonas Species by Means of the Enrichment Culture Technique
345(6)
PART 13 Bacterial Genetics 351(22)
Introduction
351(2)
Enzyme Induction
353(4)
Bacterial Conjugation
357(6)
Isolation of a Streptomycin-Resistant Mutant
363(4)
The Ames Test: A Bacterial Test System for Chemical Carcinogenicity
367(6)
PART 14 Medical Microbiology 373(62)
Introduction
373(2)
Microbial Flora of the Mouth: Determination of Susceptibility to Dental Caries
375(4)
Normal Microbial Flora of the Throat and Skin
379(4)
Identification of Human Staphylococcal Pathogens
383(8)
Identification of Human Streptococcal Pathogens
391(6)
Identification of Streptococcus pneumoniae
397(4)
Identification of Enteric Microorganisms Using Computer-Assisted Multitest Microsystems
401(10)
Enterotube Multitest System and Encise II
402(1)
API (Analytical Profile Index) System
403(8)
Isolation and Presumptive Identification of Campylobacter
411(4)
Microbiological Analysis of Urine Specimens
415(6)
Microbiological Analysis of Blood Specimens
421(6)
Species Identification of Unknown Bacterial Cultures
427(8)
PART 15 Immunology 435(54)
Introduction
435(2)
Precipitin Reaction: The Ring Test
437(4)
Precipitin Reaction: Immunodiffusion
441(6)
Agglutination Reaction: The Febrile Antibody Test
447(4)
Immunofluorescence
451(4)
Latex Agglutination Test
455(4)
Enzyme-Linked Immunoabsorbent Assay
459(4)
Agglutination Reaction: Mono-Test for Infectious Mononucleosis
463(4)
Sexually Transmitted Diseases: Rapid Immunodiagnostic Procedures
467(22)
Rapid Plasma Reagin Test for Syphilis
467(1)
Genital Herpes: Isolation and Identification of Herpes Simplex Virus
468(2)
Detection of Sexually Transmitted Chlamydial Diseases
470(3)
APPENDICES
Appendix 1: Scientific Notation
473(2)
Appendix 2: Methods for the Preparation of Dilutions
475(2)
Appendix 3: Microbiological Media
477(6)
Appendix 4: Biochemical Test Reagents
483(2)
Appendix 5: Staining Reagents
485(2)
Appendix 6: Experimental Microorganisms
487(2)
Index 489

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