Microbiology A Laboratory Manual

by Cappuccino, James; Sherman, Natalie

• ISBN13: 9780321651334
• ISBN10: 0321651332
• Edition: 9th
• Format: Spiral Bound
• Copyright: 2010-01-13
• Publisher: Benjamin Cummings

Summary

Versatile, comprehensive, and clearly written, this competitively priced laboratory manual can be used with any undergraduate microbiology book-and now features a stunning, full-color design, integrated color photographs, and newly rendered color illustrations.Microbiology: A Laboratory Manualis known for its thorough coverage, descriptive and straightforward procedures, and minimal equipment requirements. A broad range of experiments helps to convey basic principles and techniques. Each experiment includes an overview, an in-depth discussion of the principle involved, easy-to-follow procedures, and lab reports with review and critical thinking questions. Ample introductory material and laboratory safety instructions are provided. Culture Transfer Techniques; Techniques for Isolation of Pure Cultures; Cultural Characteristics of Microorganisms; Microscopic Examination of Stained Cell Preparations; Microscopic Examination of Living Microorganisms Using a Hanging-Drop Preparation or a Wet Mount; The Microscopic Measurement of Microorganisms; Preparation of Bacterial Smears; Simple Staining; Negative Staining; Gram Stain; Acid-Fast Stain (Ziehl-Neelsen Method); Differential Staining for Visualization of Bacterial Cell Structures; Nutritional Requirements: Media for the Routine Cultivation of Bacteria; Use of Differential, Selective, and Enriched Media; Physical Factors: Temperature; Physical Factors: pH of the Extracellular Environment; Physical Factors: Atmospheric Oxygen Requirements; Techniques for the Cultivation of Anaerobic Microorganisms; Serial Dilution - Agar Plate Procedure to Quantitate Viable Cells; The Bacterial Growth Curve; Extracellular Enzymatic Activities of Microorganisms; Carbohydrate Fermentation; Triple Sugar-Iron Agar Test; IMViC Test; Hydrogen Sulfide Test; Urease Test; Litmus Milk Reactions; Catalase Test; Oxidase Test; Utilization of Amino Acids; Genus Identification of Unknown Bacterial Cultures; Free-Living Protozoa; Parasitic Protozoa; Cultivation and Morphology of Molds; Yeast Morphology, Cultural Characteristics, and Reproduction; Identification of Unknown Fungi; Cultivation and Enumeration of Bacteriophages; Isolation of Coliphages from Raw Sewage; Physical Agents of Control: Moist Heat; Physical Agents of Control: Environmental Osmotic Pressure; Physical Agents of Control: Electromagnetic Radiation; Chemical Agents of Control: Chemotherapeutic Agents; Determination of Penicillin Activity in the Presence and Absence of Penicillinase; Chemical Agents of Control: Disinfectants and Antiseptics; Microbiological Analysis of Food Products: Bacterial Count; Wine Production; Standard Qualitative Analysis of Water; Quantitative Analysis of Water: Membrane Filter Method; Microbial Populations in Soil: Enumeration; Isolation of Antibiotic-Producing Microorganisms and Determination of Antimicrobial Spectrum of Isolates; Isolation of Pseudomonas Species by Means of the Enrichment Culture Technique; Enzyme Induction; Bacterial Conjugation; Isolation of a Streptomycin-Resistant Mutant; The Ames Test: A Bacterial Test System for Chemical Carcinogenicity; Bacterial Transformation; Isolation of Bacterial Plasmids; Restriction Analysis and Electrophoretic Separation of Bacteriophage Lambda DNA; Microbial Flora of the Mouth: Determination of Susceptibility to Dental Caries; Normal Microbial Flora of the Throat and Skin; Identification of Human Staphylococcal Pathogens; Identification of Human Streptococcal Pathogens; Identification of Streptococcus pneumoniae; Identification of Enteric Microorganisms Using Computer-Assisted Multitest Microsystems; Isolation and Presumptive Identification of Campylobacter; Microbiological Analysis of Urine Specimens; Microbiological Analysis of Blood Specimens; Species Identification of Unknown Bacterial Cultures ; Precipitin Reaction: The Ring Test; Agglutination Reaction: The Febrile Antibody; Immunofluorescence; Enzyme-Linked Immunoabsorbent Assay;

Author Biography

James Cappuccino is a retired instructor from the State University of New York (SUNY) system at Rockland Community College. 

Table of Contents

Part 1: Basic Laboratory Techniques for Isolation, Cultivation, and Cultural Characterization of Microorganisms
1. Culture Transfer Techniques
2. Techniques for Isolation of Pure Cultures
3. Cultural Characteristics of Microorganisms
 
Part 2: Microscopy
4. Microscopic Examination of Stained Cell Preparations
5. Microscopic Examination of Living Microorganisms Using a Hanging-Drop Preparation or a Wet Mount
6. The Microscopic Measurement of Microorganisms
 
Part 3: Bacterial Staining
7. Preparation of Bacterial Smears
8. Simple Staining
9. Negative Staining
10. Gram Stain
11. Acid-Fast Stain (Ziehl-Neelsen Method)
12. Differential Staining for Visualization of Bacterial Cell Structures
 
Part 4: Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Poulations
13. Nutritional Requirements: Media for the Routine Cultivation of Bacteria
14. Use of Differential, Selective, and Enriched Media
15. Physical Factors: Temperature
16. Physical Factors: pH of the Extracellular Environment
17. Physical Factors: Atmospheric Oxygen Requirements
18. Techniques for the Cultivation of Anaerobic Microorganisms
19. Serial Dilution - Agar Plate Procedure to Quantitate Viable Cells
20. The Bacterial Growth Curve
 
Part 5: Biochemical Activities of Microorganisms
21. Extracellular Enzymatic Activities of Microorganisms
22. Carbohydrate Fermentation
23. Triple Sugar-Iron Agar Test
24. IMViC Test
25. Hydrogen Sulfide Test
26. Urease Test
27. Litmus Milk Reactions
28. Nitrate Reduction Test
29. Catalase Test
30. Oxidase Test
31. Utilization of Amino Acids
32. Genus Identification of Unknown Bacterial Cultures
 
Part 6: The Protozoa
33. Free-Living Protozoa
34. Parasitic Protozoa
 
Part 7: The Fungi
35. Cultivation and Morphology of Molds
36. Yeast Morphology, Cultural Characteristics, and Reproduction
37. Identification of Unknown Fungi
 
Part 8: The Viruses
38. Cultivation and Enumeration of Bacteriophages
39. Isolation of Coliphages from Raw Sewage
 
Part 9: Physical and Chemical Agents for the Control of Microbial Growth
40. Physical Agents of Control: Moist Heat
41. Physical Agents of Control: Environmental Osmotic Pressure
42. Physical Agents of Control: Electromagnetic Radiation
43. Chemical Agents of Control: Chemotherapeutic Agents
44. Determination of Penicillin Activity in the Presence and Absence of Penicillinase
45. Chemical Agents of Control: Disinfectants and Antiseptics
 
Part 10: Microbiology of Food
46. Microbiological Analysis of Food Products: Bacterial Count
47. Wine Production
 
Part 11: Microbiology of Water
48. Standard Qualitative Analysis of Water
49. Quantitative Analysis of Water: Membrane Filter Method
 
Part 12: Microbiology of Soil
50. Microbial Populations in Soil: Enumeration
51. Isolation of Antibiotic-Producing Microorganisms and Determination of Antimicrobial Spectrum of Isolates
52. Isolation of Pseudomonas Species by Means of the Enrichment Culture Technique
 
Part 13: Bacterial Genetics
53. Enzyme Induction
54. Bacterial Conjugation
55. Isolation of a Streptomycin-Resistant Mutant
56. The Ames Test: A Bacterial Test System for Chemical Carcinogenicity
 
Part 14: Biotechnology
57. Bacterial Transformation
58. Isolation of Bacterial Plasmids
59. Restriction Analysis and Electrophoretic Separation of Bacteriophage Lambda DNA
 
Part 15: Medical Microbiology
60. Microbial Flora of the Mouth: Determination of Susceptibility to Dental Caries
61. Normal Microbial Flora of the Throat and Skin
62. Identification of Human Staphylococcal Pathogens
63. Identification of Human Streptococcal Pathogens
64. Identification of Streptococcus pneumoniae
65. Identification of Enteric Microorganisms Using Computer-Assisted Multitest Microsystems
66. Isolation and Presumptive Identification of Campylobacter
67. Microbiological Analysis of Urine Specimens
68. Microbiological Analysis of Blood Specimens
69. Species Identification of Unknown Bacterial Cultures
 
Part 16: Immunology
70. Precipitin Reaction: The Ring Test
71. Agglutination Reaction: The Febrile Antibody
72. Immunofluorescence
73. Enzyme-Linked Immunoabsorbent Assay
74. Agglutination Reaction: Mono-Test for Infectious Mononucleosis
75. Sexually Transmitted Diseases: Rapid Immunodiagnostic Procedures
 
Appendices