Microbiology : A Laboratory Manual

by Cappuccino, James; Sherman, Natalie

• ISBN13: 9780805325782
• ISBN10: 0805325786
• Edition: 9th
• Format: Spiral Bound
• Copyright: 2011-01-01
• Publisher: Benjamin Cummings

Summary

Key Message: Known for its straightforward and well thought-out laboratory experiments, minimal equipment requirements, and competitive price,Microbiology: A Laboratory Manual, Eighth Editionretains these advantages while gaining currency with a new "Hot Topics in Microbiology" feature, 50% new color photographs, and a new section of molecular biology experiments. This versatile laboratory manual can be used with any undergraduate microbiology text and course. Key Topics: Basic Laboratory Techniques for Isolation, Cultivation, and Cultural Characterization of Microorganisms; Microscopy; Bacterial Staining; Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Poulations; Biochemical Activities of Microorganisms; The Protozoa; The Fungi; The Viruses; Physical and Chemical Agents for the Control of Microbial Growth; Microbiology of Food; Microbiology of Water; Microbiology of Soil; Bacterial Genetics; Biotechnology; Medical Microbiology; Immunology Market: For all readers interested in microbiology.

Table of Contents

Basic Laboratory Techniques for Isolation, Cultivation, and Cultural Characterization of Microorganisms

Culture Transfer Techniques

Techniques for Isolation of Pure Cultures

Cultural Characteristics of Microorganisms

Microscopy

Microscopic Examination of Stained Cell Preparations

Microscopic Examination of Living Microorganisms Using a Hanging-Drop Preparation or a Wet Mount

The Microscopic Measurement of Microorganisms

Darkfield Microscopy

Bacterial Staining

Preparation of Bacterial Smears

Simple Staining

Negative Staining

Gram Stain

Acid-Fast Stain (Ziehl-Neelsen Method)

Differential Staining for Visualization of Bacterial Cell Structures

Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Poulations

Nutritional Requirements: Media for the Routine Cultivation of Bacteria

Use of Differential and Selective Media

Physical Factors: Temperature

Physical Factors: pH of the Extracellular Environment

Physical Factors: Atmospheric Oxygen Requirements

Techniques for the Cultivation of Anaerobic Microorganisms

Serial Dilution - Agar Plate Procedure to Quantitate Viable Cells

The Bacterial Growth Curve

Biochemical Activities of Microorganisms

Extracellular Enzymatic Activities of Microorganisms

Carbohydrate Fermentation

Triple Sugar-Iron Agar Test

IMViC Test

Hydrogen Sulfide Test

Urease Test

Litmus Milk Reactions

Nitrate Reduction Test

Catalase Test

Oxidase Test

Utilization of Amino Acids

Genus Identification of Unknown Bacterial Cultures

The Protozoa

Free-Living Protozoa

Parasitic Protozoa

The Fungi

Cultivation and Morphology of Molds

Yeast Morphology, Cultural Characteristics, and Reproduction

Identification of Unknown Fungi

The Viruses

Cultivation and Enumeration of Bacteriophages

Isolation of Coliphages from Raw Sewage

Physical and Chemical Agents for the Control of Microbial Growth

Physical Agents of Control: Moist Heat

Physical Agents of Control: Environmental Osmotic Pressure

Physical Agents of Control: Electromagnetic Radiation

Chemical Agents of Control: Chemotherapeutic Agents

Determination of Penicillin Activity in the Presence and Absence of Penicillinase

Chemical Agents of Control: Disinfectants and Antiseptics

Microbiology of Food

Microbiological Analysis of Food Products: Bacterial Count

Wine Production

Sauerkraut Production

Microbiology of Water

Standard Qualitative Analysis of Water

Quantitative Analysis of Water: Membrane Filter Method

Microbiology of Soil

Nitrogen Cycle

Microbial Populations in Soil: Enumeration

Isolation of Antibiotic-Producing Microorganisms and Determination of Antimicrobial Spectrum of Isolates

Isolation of Pseudomonas Species by Means of the Enrichment Culture Technique

Bacterial Genetics

Enzyme Induction

Bacterial Conjugation

Isolation of a Streptomycin-Resistant Mutant

The Ames Test: A Bacterial Test System for Chemical Carcinogenicity

Biotechnology

Bacterial Transformation

Isolation of Bacterial Plasmids

Restriction Analysis and Electrophoretic Separation

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