9780805325782

Microbiology : A Laboratory Manual

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  • ISBN13:

    9780805325782

  • ISBN10:

    0805325786

  • Edition: 9th
  • Format: Spiral Bound
  • Copyright: 2011-01-01
  • Publisher: Benjamin Cummings
  • View Upgraded Edition

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Summary

Key Message: Known for its straightforward and well thought-out laboratory experiments, minimal equipment requirements, and competitive price,Microbiology: A Laboratory Manual, Eighth Editionretains these advantages while gaining currency with a new "Hot Topics in Microbiology" feature, 50% new color photographs, and a new section of molecular biology experiments. This versatile laboratory manual can be used with any undergraduate microbiology text and course. Key Topics: Basic Laboratory Techniques for Isolation, Cultivation, and Cultural Characterization of Microorganisms; Microscopy; Bacterial Staining; Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Poulations; Biochemical Activities of Microorganisms; The Protozoa; The Fungi; The Viruses; Physical and Chemical Agents for the Control of Microbial Growth; Microbiology of Food; Microbiology of Water; Microbiology of Soil; Bacterial Genetics; Biotechnology; Medical Microbiology; Immunology Market: For all readers interested in microbiology.

Table of Contents

Basic Laboratory Techniques for Isolation, Cultivation, and Cultural Characterization of Microorganisms
Culture Transfer Techniques
Techniques for Isolation of Pure Cultures
Cultural Characteristics of Microorganisms
Microscopy
Microscopic Examination of Stained Cell Preparations
Microscopic Examination of Living Microorganisms Using a Hanging-Drop Preparation or a Wet Mount
The Microscopic Measurement of Microorganisms
Darkfield Microscopy
Bacterial Staining
Preparation of Bacterial Smears
Simple Staining
Negative Staining
Gram Stain
Acid-Fast Stain (Ziehl-Neelsen Method)
Differential Staining for Visualization of Bacterial Cell Structures
Cultivation of Microorganisms: Nutritional and Physical Requirements, and Enumeration of Microbial Poulations
Nutritional Requirements: Media for the Routine Cultivation of Bacteria
Use of Differential and Selective Media
Physical Factors: Temperature
Physical Factors: pH of the Extracellular Environment
Physical Factors: Atmospheric Oxygen Requirements
Techniques for the Cultivation of Anaerobic Microorganisms
Serial Dilution - Agar Plate Procedure to Quantitate Viable Cells
The Bacterial Growth Curve
Biochemical Activities of Microorganisms
Extracellular Enzymatic Activities of Microorganisms
Carbohydrate Fermentation
Triple Sugar-Iron Agar Test
IMViC Test
Hydrogen Sulfide Test
Urease Test
Litmus Milk Reactions
Nitrate Reduction Test
Catalase Test
Oxidase Test
Utilization of Amino Acids
Genus Identification of Unknown Bacterial Cultures
The Protozoa
Free-Living Protozoa
Parasitic Protozoa
The Fungi
Cultivation and Morphology of Molds
Yeast Morphology, Cultural Characteristics, and Reproduction
Identification of Unknown Fungi
The Viruses
Cultivation and Enumeration of Bacteriophages
Isolation of Coliphages from Raw Sewage
Physical and Chemical Agents for the Control of Microbial Growth
Physical Agents of Control: Moist Heat
Physical Agents of Control: Environmental Osmotic Pressure
Physical Agents of Control: Electromagnetic Radiation
Chemical Agents of Control: Chemotherapeutic Agents
Determination of Penicillin Activity in the Presence and Absence of Penicillinase
Chemical Agents of Control: Disinfectants and Antiseptics
Microbiology of Food
Microbiological Analysis of Food Products: Bacterial Count
Wine Production
Sauerkraut Production
Microbiology of Water
Standard Qualitative Analysis of Water
Quantitative Analysis of Water: Membrane Filter Method
Microbiology of Soil
Nitrogen Cycle
Microbial Populations in Soil: Enumeration
Isolation of Antibiotic-Producing Microorganisms and Determination of Antimicrobial Spectrum of Isolates
Isolation of Pseudomonas Species by Means of the Enrichment Culture Technique
Bacterial Genetics
Enzyme Induction
Bacterial Conjugation
Isolation of a Streptomycin-Resistant Mutant
The Ames Test: A Bacterial Test System for Chemical Carcinogenicity
Biotechnology
Bacterial Transformation
Isolation of Bacterial Plasmids
Restriction Analysis and Electrophoretic Separation
Table of Contents provided by Publisher. All Rights Reserved.

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