PCR Protocols in Molecular Toxicology

by Vanden Heuvel; John P.

• ISBN13: 9780849333446
• ISBN10: 084933344X
• Format: Nonspecific Binding
• Copyright: 1997-09-24
• Publisher: CRC Press

Summary

Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail:o Quantitative reverse transcriptase PCR and methods to examine gene expressiono Differential display cloningo Cloning and library screening by PCRo Genotype and polymorphism analysis of drug and toxicant metabolizing enzymeso Basic, non-PCR based molecular biology methodsPCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.

Table of Contents

Chapter 1. The Basics of the Polymerase Chain Reaction	1	(40)
William B. Mattes
Chapter 2. Analysis of Gene Expression	41	(58)
John P. Vanden Heuvel
Chapter 3. Differential Display PCR	99	(22)
J. Christopher Corton
Chapter 4. Cloning by PCR	121	(42)
John P. Vanden Heuvel
Chapter 5. Genotype Analysis	163	(14)
Douglas A. Bell
Gary Pittman
Chapter 6. General Molecular Biology Techniques	177	(36)
John P. Vanden Heuvel
Appendix I -- List of Suppliers	213	(4)
Appendix II -- Companies, Online Catalogs, and Internet Resources	217	(2)
Index	219