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9780306460937

Principles of Fluorescence Spectroscopy

by Lakowicz, J.
  • ISBN13:

    9780306460937

  • ISBN10:

    0306460939

  • eBook ISBN(s):

    9781475730616

  • Additional ISBN(s):

    9780387312781

  • Edition: 2nd
  • Format: Hardcover
  • Copyright: 1999-07-01
  • Publisher: Kluwer Academic Pub
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Summary

'In the second edition of Principles I have attempted to maintain the emphasis on basics, while updating the examples to include more recent results from the literature. There is a new chapter providing an overview of extrinisic fluorophores. The discussion of timeresolved measurements has been expanded to two chapters. Quenching has also been expanded in two chapters. Energy transfer and anisotropy have each been expanded to three chapters. There is also a new chapter on fluorescence sensing. To enhance the usefulness of this book as a textbook, most chapters are followed by a set of problems. Sections which describe advanced topics are indicated as such, to allow these sections to be skipped in an introduction course. Glossaries are provided for commonly used acronyms and mathematical symbols. For those wanting additional informtion, the final appendix contains a list of recommended books which expand on various specialized topics.' from the author's Preface

Table of Contents

Introduction to Fluorescence
Instrumentation for Fluorescence Spectroscopy
Fluorophores
Time-Domain Lifetime Measurements
Frequency-Domain Lifetime Measurements
Solvent Effects on Emission Spectra
Dynamics of Solvent and Spectral Relaxation
Quenching of Fluorescence
Advanced Topics in Fluorescence Quenching
Fluorescence Anisotropy
Time-Dependent Anisotropy Decays
Advanced Anisotropy Concepts
Energy Transfer
Time-Resolved Energy Transfer and Conformational Distributions of Biopolymers
Energy Transfer to Multiple Acceptors in One, Two or Three-Dimensions
Protein Fluorescence
Time-Resolved Protein Fluorescence
Excited State Reactions
Fluorescence Sensing
Long Lifetime Metal-Ligand Complexes
DNA Technology
Phase Sensitive and Phase Resolved Emission Spectra
Appendixes
Corrected Emission Spectra
Fluorescent Lifetime Standards
Additional Reading
Index
Table of Contents provided by Publisher. All Rights Reserved.

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