Protein Purification : Principles, High Resolution Methods, and Applications

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  • Edition: 3rd
  • Format: Hardcover
  • Copyright: 2011-03-22
  • Publisher: Wiley

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This thoroughly revised and updated third edition provides an overview of the principles and methodology of the most frequently used biochemical separation techniques for proteins used today. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including: Gel filtration and ion exchange chromatography Affinity and covalent chromatography Reversed phase and hydrophobic interaction chromatography Ligands from combinatorial libraries Electrophoresis in gels Immunoelectrophoresis and capillary electrophoresis Two-dimensional electrophoresis in proteomics Protein elution and blotting techniques Large-scale electrophoresis processes

Author Biography

JAN-CHRISTER JANSON is Professor Emeritus at the Department of Physical and Analytical Chemistry, Uppsala University, Uppsala, Sweden.

Table of Contents

Introduction to Protein Purification
Introduction to Chromatography
Gel Filtration
Ion Exchange Chromatography
High-Resolution Reversed-Phase Chromatography
Hydrophobic Interaction Chromatography
Immobilized Metal Ion Affinity Chromatography
Covalent Chromatpgraphy
Affinity Chromatography
Affinity Ligands from Chemical Combinatorial Libraries
Affinity Ligands from Biological Combinatorial Libraries
Other Separation Methods and Related Techniques
Membrane Separations
Refolding of Inclusion Body Proteins from E. coli
Purification of PEGylated Proteins
Electrophoresis in Gels
Conventional Isoelectric Focusing in Gel Slabs and Capillaries and Immobilized pH Gradients
Two-Dimensional Electrophoresis in Proteomics
Protein Elution and Blotting Techniques
Capillary Electrophoretic Separations
Separation Method Optimization
High Throughput Screening Techniques on Protein Purification
Table of Contents provided by Publisher. All Rights Reserved.

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