Scanning Electron Microscopy for the Life Sciences

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  • Format: Hardcover
  • Copyright: 2013-01-28
  • Publisher: Cambridge University Press
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Recent developments in scanning electron microscopy (SEM) have resulted in a wealth of new applications for cell and molecular biology, as well as related biological disciplines. It is now possible to analyze macromolecular complexes within their three-dimensional cellular microenvironment in near native states at high resolution, and to identify specific molecules and their structural and molecular interactions. New approaches include cryo-SEM applications and environmental SEM (ESEM), staining techniques and processing applications combining embedding and resin-extraction for imaging with high resolution SEM, and advances in immuno-labeling. New developments include helium ion microscopy, automated block-face imaging combined with serial sectioning inside an SEM chamber, and Focused Ion Beam Milling (FIB) combined with block-face SEM. With chapters written by experts, this guide gives an overview of SEM and sample processing for SEM, and highlights several advances in cell and molecular biology that greatly benefited from using conventional, cryo, immuno, and high-resolution SEM.

Table of Contents

The role of scanning electron microscopy in cell and molecular biology: SEM basics, past accomplishments and new frontiers
Corrosion casting technique Jerzy Walocha, Jan
Revealing the internal structure of cells in three dimensions with scanning electron microscopy
Mitochondria form continuous intracellular network-structures visualized with high-resolution field-emission scanning electron microscopy
Chapter on 3-D reconstruction of cell organelles using STEM tomography
High resolution labeling for correlative microscopy
The use of SEM to explore virus structure and trafficking
High resolution scanning electron microscopy of the nuclear surface in Herpes Simplex Virus 1 infected cells
Scanning electron microscopy of chromosomes: structural and analytical investigations
A method to visualize the microarchitecture of glycoprotein matrices with scanning electron microscopy
Scanning electron microscopy of cerebellar intrinsic circuits
Application of in vivo cryotechnique to living animal organs examined by scanning electron microscopy
SEM in dental research
SEM, teeth and palaeoanthropology: the secret of ancient human diets
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