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9781588294852

Myeloid Leukemia

by ; ;
  • ISBN13:

    9781588294852

  • ISBN10:

    1588294854

  • Format: Hardcover
  • Copyright: 2005-11-30
  • Publisher: Humana Pr Inc

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Summary

A panel of internationally recognized research scientists and clinical investigators brings together a diverse collection of readily reproducible methods for identifying and quantifying a large number of specific genetic abnormalities associated with the broad spectrum of myeloid malignancies. Highlights include techniques for the detection of BCR-ABL mutations and resistance to imatinib mesylate, detection of the FIP1L1-PDGFRA fusion in idiopathic hypereosinophilic syndrome and chronic eosinophilic leukemia, classification of AML by DNA-oligonucleotide microarrays, and detection of the V617F JAK2 mutation in myeloproliferative disorders. In addition to gene rearrangments, other prognostically relevant molecular lesions such as FLT3 mutations and WT-1 overexpression are covered.

Table of Contents

Preface v
Contributors ix
Isolation of RNA and DNA From Leukocytes and cDNA Synthesis
1(12)
Joop H. Jansen
Bert A. van der Reijden
Cytogenetic and FISH Techniques in Myeloid Malignancies
13(14)
Lynda J. Campbell
Overview of Real-Time RT-PCR Strategies for Quantification of Gene Rearrangements in the Myeloid Malignancies
27(42)
Christophe Picard
Monique Silvy
Jean Gabert
Diagnosis and Monitoring of Chronic Myeloid Leukemia by Qualitative and Quantitative RT-PCR
69(24)
Susan Branford
Timothy Hughes
Detection of BCR-ABL Mutations and Resistance to Imatinib Mesylate
93(14)
Susan Branford
Timothy Hughes
Deletion of the Derivative Chromosome 9 in Chronic Myeloid Leukemia
107(8)
Lynda J. Campbell
Diagnosis and Monitoring of PML-RARA-Positive Acute Promyelocytic Leukemia by Qualitative RT-PCR
115(12)
Vincenzo Rossi
Laura Levati
Andrea Biondi
Diagnosis and Monitoring of PML-RARα-Positive Acute Promyelocytic Leukemia by Quantitative RT-PCR
127(22)
Elisa Mokany
Alison V. Todd
Caroline J. Fuery
Tanya L. Applegate
Diagnosis and Monitoring of AML1-MTG8 (ETO)-Positive Acute Myeloid Leukemia by Qualitative and Real-Time Quantitative RT-PCR
149(14)
Khalid Tobal
John A. Liu Yin
Diagnosis and Monitoring of CBFB-MYH11-Positive Acute Myeloid Leukemia by Qualitative and Quantitative RT-PCR
163(14)
Bert A. van der Reijden
Joop H. Jansen
Detection of the FIP1L1-PDGFRA Fusion in Idiopathic Hypereosinophilic Syndrome and Chronic Eosinophilic Leukemia
177(12)
Jan Cools
Elizabeth H. Stover
D. Gary Gilliland
FLT3 Mutations in Acute Myeloid Leukemia
189(10)
Hitoshi Kiyoi
Tomoki Naoe
WT-1 Overexpression in Acute Myeloid Leukemia and Myelodysplastic Syndromes
199(14)
Daniela Cilloni
Enrico Gottardi
Giuseppe Saglio
Classification of AML by DNA-Oligonucleotide Microarrays
213(28)
Alexander Kohlmann
Wolfgang Kern
Wolfgang Hiddemann
Torsten Haferlach
Classification of AML Using a Monoclonal Antibody Microarray
241(12)
Richard I. Christopherson
Kerryn Stoner
Nicole Barber
Larissa Belov
Adrian Woolfson
Mike Scott
Linda Bendall
Stephen P. Mulligan
Methods for the Detection of the JAK2 V617F Mutation in Human Myeloproliferative Disorders
253(12)
Peter J. Campbell
Linda M. Scott
E. Joanna Baxter
Anthony J. Bench
Anthony R. Green
Wendy N. Erber
Overexpression of PRV-1 Gene in Polycythemia Rubra Vera and Essential Thrombocythemia
265(10)
Maurizio Martini
Luciana Teofili
Luigi M. Larocca
Chimerism Analysis Following Nonmyeloablative Stem Cell Transplantation
275(22)
Thomas Lion
Franz Watzinger
Index 297

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