Transgenic Animal Technology : A Laboratory Handbook

by Pinkert, Carl A.

• ISBN13: 9780125571654
• ISBN10: 0125571658
• Format: Paperback
• Copyright: 1994-01-01
• Publisher: Academic Pr

Summary

Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer from molecular methods to whole animal considerations for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems.

Table of Contents

Contributors	ix
Preface	xi
PART I Overview
Introduction to Transgenic Animals	3	(12)
Carl A. Pinkert
Introduction	3	(1)
Historical Background	4	(3)
Applications and Overview of Text	7	(8)
References	11	(4)
PART II Transgenic Animal Production Focusing on the Mouse Model
DNA Microinjection and Transgenic Animal Production	15	(54)
H. Greg Polites
Carl A. Pinkert
Introduction	15	(1)
General Methods	16	(42)
Summary	58	(11)
Appendix	59	(8)
References	67	(2)
Factors Affecting Transgenic Animal Production	69	(46)
Paul A. Overbeek
Introduction	70	(1)
Getting Started	70	(20)
Troubleshooting	90	(6)
Transgenic Phenomenology	96	(11)
Summary	107	(8)
Appendix: Standardized Nomenclature for Transgenic Animals	107	(5)
References	112	(3)
Gene Transfer in Embryonic Stem Cells	115	(32)
Thomas Doetschman
Introduction to Embryonic Stem Cells	115	(3)
Gene Transfer and Expression	118	(7)
Gene Targeting in Embryonic Stem Cells	125	(8)
Methods	133	(14)
References	140	(7)
Retrovirus-Mediated Gene Transfer	147	(30)
Philip A. Wood
Introduction	147	(1)
Applications	148	(1)
General Methods	148	(14)
Specific Applications for Retroviral Transgene Expression in Vivo	162	(9)
Summary	171	(6)
References	171	(6)
Gene Transfer Technology: Alternative Techniques and Applications	177	(44)
Glenn M. Monastersky
Introduction of Transgenes into Gametes and Embryos	178	(5)
Somatic Cell Transformation	183	(18)
Strategies in Molecular Medicine	201	(20)
References	209	(12)
Molecular Approaches Involved in Mammalian Gene Transfer: Analysis of Transgene Integration	221	(14)
Brad T. Tinkle
Charles J. Bieberich
Gilbert Jay
Introduction	221	(1)
Detection of the Transgene	222	(1)
Parameters for Evaluating a Transgene	223	(1)
Analysis of the Transgene	224	(5)
Establishing a Transgenic Line	229	(2)
Derivation of Homozygous Mice	231	(2)
Summary	233	(2)
References	233	(2)
Molecular Approaches Involved in Mammalian Gene Transfer: Evaluation of Transgene Expression	235	(30)
Charles J. Bieberich
Lien Ngo
Gilbert Jay
Introduction	235	(3)
Analyses of Steady-State Levels of Transgenic RNA Transcripts	238	(11)
Analyses of Steady-State Levels of Transgenic Protein Products	249	(10)
Conclusions	259	(6)
References	260	(5)
PART III Production of Transgenic Laboratory and Domestic Animal Species
Production of Transgenic Rats and Rabbits	265	(14)
James M. Robl
Jan K. Heideman
Introduction	265	(2)
Materials and Methods	267	(12)
References	275	(4)
Production of Transgenic Poultry and Fish	279	(36)
Linda C. Cioffi
John J. Kopchick
Howard Y. Chen
Introduction	279	(1)
Transgenic Poultry	280	(17)
Transgenic Fish	297	(18)
References	308	(7)
Production of Transgenic Swine	315	(24)
Michael J. Martin
Carl A. Pinkert
Introduction and Discussion	315	(5)
Methods	320	(12)
Summary	332	(7)
References	333	(6)
Production of Transgenic Ruminants	339	(18)
Caird E. Rexroad, Jr.
Harold W. Hawk
Introduction	339	(1)
Transgenic Cattle	340	(6)
Transgenic Sheep and Goats	346	(7)
Summary	353	(4)
References	354	(3)
Index	357