9780125571654

Transgenic Animal Technology : A Laboratory Handbook

by
  • ISBN13:

    9780125571654

  • ISBN10:

    0125571658

  • Format: Paperback
  • Copyright: 1994-01-01
  • Publisher: Academic Pr
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Supplemental Materials

What is included with this book?

Summary

Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer from molecular methods to whole animal considerations for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engineering technologies ranging from microinjection and ES cell transfer to nuclear transfer in a broad range of animal modeling systems.

Table of Contents

Contributors ix
Preface xi
PART I Overview
Introduction to Transgenic Animals
3(12)
Carl A. Pinkert
Introduction
3(1)
Historical Background
4(3)
Applications and Overview of Text
7(8)
References
11(4)
PART II Transgenic Animal Production Focusing on the Mouse Model
DNA Microinjection and Transgenic Animal Production
15(54)
H. Greg Polites
Carl A. Pinkert
Introduction
15(1)
General Methods
16(42)
Summary
58(11)
Appendix
59(8)
References
67(2)
Factors Affecting Transgenic Animal Production
69(46)
Paul A. Overbeek
Introduction
70(1)
Getting Started
70(20)
Troubleshooting
90(6)
Transgenic Phenomenology
96(11)
Summary
107(8)
Appendix: Standardized Nomenclature for Transgenic Animals
107(5)
References
112(3)
Gene Transfer in Embryonic Stem Cells
115(32)
Thomas Doetschman
Introduction to Embryonic Stem Cells
115(3)
Gene Transfer and Expression
118(7)
Gene Targeting in Embryonic Stem Cells
125(8)
Methods
133(14)
References
140(7)
Retrovirus-Mediated Gene Transfer
147(30)
Philip A. Wood
Introduction
147(1)
Applications
148(1)
General Methods
148(14)
Specific Applications for Retroviral Transgene Expression in Vivo
162(9)
Summary
171(6)
References
171(6)
Gene Transfer Technology: Alternative Techniques and Applications
177(44)
Glenn M. Monastersky
Introduction of Transgenes into Gametes and Embryos
178(5)
Somatic Cell Transformation
183(18)
Strategies in Molecular Medicine
201(20)
References
209(12)
Molecular Approaches Involved in Mammalian Gene Transfer: Analysis of Transgene Integration
221(14)
Brad T. Tinkle
Charles J. Bieberich
Gilbert Jay
Introduction
221(1)
Detection of the Transgene
222(1)
Parameters for Evaluating a Transgene
223(1)
Analysis of the Transgene
224(5)
Establishing a Transgenic Line
229(2)
Derivation of Homozygous Mice
231(2)
Summary
233(2)
References
233(2)
Molecular Approaches Involved in Mammalian Gene Transfer: Evaluation of Transgene Expression
235(30)
Charles J. Bieberich
Lien Ngo
Gilbert Jay
Introduction
235(3)
Analyses of Steady-State Levels of Transgenic RNA Transcripts
238(11)
Analyses of Steady-State Levels of Transgenic Protein Products
249(10)
Conclusions
259(6)
References
260(5)
PART III Production of Transgenic Laboratory and Domestic Animal Species
Production of Transgenic Rats and Rabbits
265(14)
James M. Robl
Jan K. Heideman
Introduction
265(2)
Materials and Methods
267(12)
References
275(4)
Production of Transgenic Poultry and Fish
279(36)
Linda C. Cioffi
John J. Kopchick
Howard Y. Chen
Introduction
279(1)
Transgenic Poultry
280(17)
Transgenic Fish
297(18)
References
308(7)
Production of Transgenic Swine
315(24)
Michael J. Martin
Carl A. Pinkert
Introduction and Discussion
315(5)
Methods
320(12)
Summary
332(7)
References
333(6)
Production of Transgenic Ruminants
339(18)
Caird E. Rexroad, Jr.
Harold W. Hawk
Introduction
339(1)
Transgenic Cattle
340(6)
Transgenic Sheep and Goats
346(7)
Summary
353(4)
References
354(3)
Index 357

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