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9781588291400

Vaccine Protocols

by ; ;
  • ISBN13:

    9781588291400

  • ISBN10:

    1588291405

  • Edition: 2nd
  • Format: Hardcover
  • Copyright: 2003-09-01
  • Publisher: Humana Pr Inc

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Supplemental Materials

What is included with this book?

Summary

This extensively revised, updated, and expanded second edition of a classic laboratory manual applies new understanding of molecular mechanisms to the rational design and development of vaccines. Here the working researcher will find readily reproducible techniques for the development and production of live attenuated vaccines, and vaccines based on inactivated toxins, peptides, and polysaccharide conjugates. New to this edition are cutting-edge chapters on two different viral vectors (Vaccinia and Semliki Forest), lactic acid bacteria for delivery of foreign antigens, incorporation of immunomodulators into plasmid DNA vaccines, construction of tetramer complexes to detect CMI responses, assessment of functional antibody responses, and the use of complete genome sequences for vaccine design. There are also chapters describing more general techniques for vaccine formulation, delivery, and assessment of immune responses, and review discussions of scale-up to manufacture, vaccine quality assurance, and clinical trials.

Table of Contents

Preface v
Contributors ix
1 Overview of Vaccines
Gordon Ada
1(18)
2 Temperature-Sensitive Mutant Vaccines
Craig R. Pringle
19(18)
3 Live Viral Vectors: Construction of a Replication-Deficient Recombinant Adenovirus
Anthony R. Fooks
37(14)
4 Live Viral Vectors: Vaccinia Virus
Caroline Staib and Gerd Sutter
51(18)
5 Live Viral Vectors: Semliki Forest Virus
Gunilla B. Karlsson and Peter J. Liljeström
69(14)
6 Development of Attenuated Salmonella Strains That Express Heterologous Antigens
Frances Bowe, Derek J. Pickard, Richard J. Anderson, Patricia Londoño-Ardila, and Gordon Dougan
83(18)
7 Expression and Delivery of Heterologous Antigens Using Lactic Acid Bacteria
Mark A. Reuter, Sean Hanniffy, and Jerry M. Wells
101(14)
8 Synthetic Peptides
Michael J. Francis
115(18)
9 Genetic Detoxification of Bacterial Toxins
Mariagrazia Pizza, Maria Rita Fontana, Vincenzo Scarlato, and Rino Rappuoli
133(20)
10 Preparation of Polysaccharide-Conjugate Vaccines
Carla C.A.M. Peeters, Patrick R. Lagerman, Odo de Weers, Lukas A. Oomen, Peter Hongerhout, Michel Beurret, Karen M. Reddin, and Jan T. Poolman
153(22)
11 Adjuvant Formulations for Experimental Vaccines
Duncan E. S. Stewart-Tull
175(20)
12 Incorporation of Immunomodulators into Plasmid DNA Vaccines
Linda S. Klavinskis, Philip Hobson, and Andrew Woods
195(16)
13 Microencapsulation of Vaccine Antigens
David H. Jones
211(12)
14 Lyophilization of Vaccines: Current Trends
Gerald D.J. Adams
223(22)
15 Stimulation of Mucosal Immunity
David J.M. Lewis and Christopher M.M. Hayward
245(10)
16 Induction and Detection of T-Cell Responses
Kingston H.G. Mills
255(24)
17 Construction of MHC Class 1-Peptide Tetrameric Complexes for Analysis of T-Cell-Mediated Immune Responses
Rachel V. Samuel and Tomás Hanke
279(10)
18 Assessment of Functional Antibody Responses
Ray Borrow and Paul Balmer
289(12)
19 The Use of Complete Genome Sequences in Vaccine Design
Nigel J. Saunders and Sarah Butcher
301(12)
20 Severe Combined Immunodeficient (SCID) Mice in Vaccine Assessment
Michael J. Dennis
313(22)
21 Clinical Trials
Paddy Farrington and Elizabeth Miller
335(18)
22 Assuring the Quality and Safety of Vaccines: Regulatory Expectations for Licensing and Batch Release
Elwyn Griffiths and Ivana Knezevic
353(24)
23 DNA Vaccination: An Update
Douglas B. Lowrie
377(14)
24 From Vaccine Research to Manufacture: A Guide for the Researcher
Nigel Allison and Howard S. Tranten
391(18)
Index 409

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