Analytical Ultracentrifugation

by Scott, David John; Harding, Stephen E.; Rowe, Arthur J.

• ISBN13: 9780854045471
• ISBN10: 0854045473
• Format: Hardcover
• Copyright: 2006-03-30
• Publisher: Royal Society of Chemistry

Summary

"A must for all biochemists and biophysicists." Jayesh Gor, CHEMISTRY WORLD, July 2006 Analytical Ultracentrifugation has become an increasingly important technique for monitoring the size and shape of biological macromolecules. "Analytical Ultracentrifugation: Techniques and Methods" contains contributions from experts in the field, bringing together the multitude of developments that have taken place in instrumentation and analysis over the past decade into a single volume. This book covers the latest methods in analysis along with an extensive introduction for the novice user. Analysis methods in both sedimentation velocity and sedimentation equilibrium are discussed at length. Protein, Protein/DNA, membrane proteins and polymer systems are also explored, along with software developments and non-ideality.

Table of Contents

A brief introduction to the analytical ultracentrifugation of proteins for beginners	p. 1
Diffusion-deconvoluted sedimentation coefficient distributions for the analysis of interacting and non-interacting protein mixtures	p. 26
Models for direct boundary fitting of indefinite ligand-linked self-association	p. 51
Allowance for thermodynamic nonideality in sedimentation equilibrium	p. 64
Using analytical ultracentrifugation of DNA in CsCl gradients to explore large-scale properties of genomes	p. 104
Lammnum : a program to study self-associating macromolecules in sedimentation velocity experiments	p. 122
Correcting for the buoyancy of macromolecules : density increments and apparent partial specific volumes with particular reference to the study of membrane proteins	p. 133
Application of weighted robust regression to equilibrium ultracentrifugation	p. 152
The synthetic boundary technique of analytical ultracentrifugation : a valuable tool to study structure formation processes online	p. 162
Protein-DNA interactions studied at sedimentation equilibrium	p. 195
UltraScan - a comprehensive data analysis software package for analytical ultracentrifugation experiments	p. 230
Analysis of polysaccharide size, shape and interactions	p. 231
Analytical ultracentrifugation as a key complementary technique in structural biology	p. 252
Introduction to differential sedimentation	p. 270
Relating small angle scattering and analytical ultracentrifugation in multidomain proteins	p. 291
Modelling of biopolymers	p. 320
Fitting of thermodynamic data from equilibrium ultracentrifugation by robust least-squares with reduced parameter cross-correlation coefficients	p. 372
Calculation of volume, surface, and hydration properties of biopolymers	p. 389
Analysis of membrane proteins using analytical ultracentrifugation	p. 432
Solution properties of flexible macromolecules : theoretical and computational approaches	p. 449
The ELLIPS suite of whole-body protein conformation algorithms for Microsoft Windows	p. 468
Weak interactions : optimal algorithms for their study in the AUC	p. 484
Analytical ultracentrifugation of colloids	p. 501
Table of Contents provided by Blackwell. All Rights Reserved.