Animal Cell Culture Essential Methods

by Davis, John M.

• ISBN13: 9780470666586
• ISBN10: 0470666587
• Edition: 1st
• Format: Paperback
• Copyright: 2011-03-21
• Publisher: Wiley

Summary

This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.This will be the third book in the Essential Methods Series, taken on from Scion Publishing.

Author Biography

Dr John Davis: Visiting Lecturer in Biotechnology, University of Hertfordshire, previously Senior Project Scientist at Bio Products Laboratory, UK.

Table of Contents

Animal Cell Culture - Essential Methods

The Cell Culture Laboratory

Sterilization

The Microscopy of Living Cells

Basic Techniques and Media, the Maintenance of Cell Lines, and Safety

Serum-Free Culture Media

Cryopreservation and Cell Banking

Primary Culture of Specific Cell Types, and the Establishment of Cell Lines

Cloning

The Quality Control of Cell Lines and the Prevention, Detection and Cure of Contamination

Systems for Culture Scale-Up

Good Laboratory Practice (GLP) in the Cell Culture Laboratory

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