Bacterial Pathogenesis

by Deleo, Frank R.; Otto, Michael

• ISBN13: 9781588297402
• ISBN10: 1588297403
• Edition: 1st
• Format: Hardcover
• Copyright: 2008-02-01
• Publisher: Humana Pr Inc

Summary

Bacterial infections affect world health today as a leading cause of morbidity and mortality. This book presents in-depth methods and state-of-the-art protocols for investigating specific mechanisms of pathogenesis for a wide range of bacteria. Written by experts in the field, this invaluable collection includes protocols to study host-pathogen interactions, animal models of infection, and novel approaches to identifying therapeutic targets designed to control infections.

Table of Contents

Preface	p. v
Contributors	p. ix
List of Color Plates	p. xiii
Mechanisms of Bacterial Pathogenesis
Isolation and Characterization of Lipopolysaccharides	p. 3
Proteomic Analysis of Proteins Secreted by Streptococcus pyogenes	p. 15
Proteomic Analysis to Investigate Regulatory Networks in Staphylococcus aureus	p. 25
Microarray Comparative Genomic Hybridization for the Analysis of Bacterial Population Genetics and Evolution	p. 47
Detection and Inhibition of Bacterial Cell-Cell Communication	p. 55
A System for Site-Specific Genetic Manipulation of the Relapsing Fever Spirochete Borrelia hermsii	p. 69
Transposon Mutagenesis of the Lyme Disease Agent Borrelia burgdorferi	p. 85
The Biofilm Exopolysaccharide Polysaccharide Intercellular Adhesin-A Molecular and Biochemical Approach	p. 97
Host-Pathogen Interaction
Analysis of Staphylococcus aureus Gene Expression During PMN Phagocytosis	p. 109
Examining the Vector-Host-Pathogen Interface With Quantitative Molecular Tools	p. 123
Intracellular Localization of Brucella abortus and Francisella tularensis in Primary Murine Macrophages	p. 133
Rate and Extent of Helicobacter pylori Phagocytosis	p. 147
Culture, Isolation, and Labeling of Anaplasma phagocytophilum for Subsequent Infection of Human Neutrophils	p. 159
Ultrastructural Analysis of Bacteria-Host Cell Interactions	p. 173
Infection of Human Monocyte-Derived Macrophages With Coxiella burnetii	p. 189
Infection of Epithelial Cells With Salmonella enterica	p. 201
Determining the Cellular Targets of Reactive Oxygen Species in Borrelia burgdorferi	p. 213
Animal Models of Bacterial Infection
Bioluminescent Imaging of Bacterial Biofilm Infections in Vivo	p. 225
The Cotton Rat as a Model for Staphylococcus aureus Nasal Colonization in Humans: Cotton Rat S. aureus Nasal Colonization Model	p. 241
A Non-Human Primate Model of Acute Group A Streptococcus Pharyngitis	p. 255
Identification of Therapeutic Targets and Typing Methods
Target-Based Antimicrobial Drug Discovery	p. 271
Sequence Analysis of the Variable Number Tandem Repeat in Staphylococcus aureus Protein A Gene: spa Typing	p. 285
Index	p. 307
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