Basic Methods in Microscopy Protocols and Concepts from Cells: A Laboratory Manual

by Spector, David L.; Goldman, Robert D.

• ISBN13: 9780879697471
• ISBN10: 0879697474
• Edition: 1st
• Format: Hardcover
• Copyright: 2005-10-26
• Publisher: Cold Spring Harbor Laboratory Press

Summary

Imaging has become a vital tool for researchers in all aspects of biology. Recent advances in microscope technology, labeling techniques and gene and protein manipulation methods have led to breakthroughs in our understanding of biological processes. In order to take advantage of these techniques, biologists need to understand the fundamental techniques of microscopy. The methods found here, drawn from the popular laboratory standard manual Cells: A Laboratory Manual,provide a solid course in the basics of using the microscope in a biology laboratory. Basic Methods in Microscopyprovides an essential guide to light microscopy, fluorescence microscopy, confocal microscopy, multiphoton microscopy and electron microscopy, preparation of tissues and cells, labeling of specimens and analysis of cellular events. This manual is an important tool for any biology researcher employing imaging as a research method.

Table of Contents

Preface	ix
Light Microscopy	1	(42)
Ernst Keller
Robert D. Goldman
Confocal Microscopy, Deconvolution, and Structured Illumination Methods	43	(40)
John M. Murray
Multiphoton and Multispectral Laser-scanning Microscopy	83	(22)
Mary E. Dickinson
Preparation of Cells and Tissues for Fluorescence Microscopy	105	(20)
Andrew H. Fischer
Kenneth A. Jacobson
Jack Rose
Rolf Zeller
Nonimmunological Fluorescent Labeling of Cellular Structures	125	(20)
Brad Chazotte
Introduction to Immunofluorescence Microscopy	145	(10)
George McNamara
Immunostaining of Microtubules, Microtubule-associated Proteins, and Intermediate Filaments	155	(10)
Satya Khoun
Joanna Olmsted
Immunofluorescence Localization of Actin	165	(6)
Anna Spudich
Immunofluorescence Localization of Nuclear Proteins	171	(4)
David L. Spector
Immunofluorescence Methods for Saccharomyces cerevisiae	175	(8)
Pamela Silver
Immunofluorescence Methods for Drosophila Tissues	183	(11)
Jason Swedlow
Immunofluorescence Methods for Caenorhabditis elegans	194	(7)
Sarah Crittenden
Judith Kimble
Analyzing DNA Replication: Nonisotopic Labeling	201	(12)
Dean Jackson
Peter R. Cook
Analyzing RNA Synthesis: Nonisotopic Labeling	213	(10)
Dean Jackson
Peter R. Cook
Fluorescence In Situ Hybridization to DNA	223	(38)
Peter Lichter
Stefan Lampen
Johanna Bridger
Whole-Mount Fluorescence In Situ Hybridization to Drosophila Chromosomal DNA	261	(16)
Abby Dernburg
In Situ Hybridization to RNA	277	(14)
Sui Huang
Robert Singer
Whole-Mount In Situ Detection of RNAs in Vertebrate Embryos and Isolated Organs	291	(12)
Anna Haramis
Rolf Zeller
Image Production Using Transmission Electron Microscopy	303	(4)
Mark H. Ellisman
Preparative Methods for Transmission Electron Microscopy	307	(36)
Thomas J. Deerinck
Maryann Martone
Mark H. Ellisman
Immunoelectron Microscopy	343	(16)
Robert Ochs
Appendix 1 Microscopy: Lenses, Filters, and Emission/Excitation Spectra	359	(10)
Appendix 2 Cautions	369	(10)
Index	379