Benson's Microbiological Applications : Laboratory Manual in General Microbiology, Complete Version

by Benson, Harold J.

• ISBN13: 9780072992731
• ISBN10: 0072992735
• Edition: 10th
• Format: Paperback
• Copyright: 2006-03-31
• Publisher: McGraw-Hill Science/Engineering/Math

Summary

The classic resource for undergraduate microbiology laboratory courses just keeps getting better. The self-contained, clearly illustrated exercises and full-color format make Microbiological Applications: Laboratory Manual in General Microbiology the ideal lab manual. Appropriate for either a majors or non-majors lab course, this manual assumes no prior organic chemistry course has been taken.

Table of Contents

Part 1 Microscopy

Exercise 1 Brightfield Microscopy

Exercise 2 Darkfield Microscopy

Exercise 3 Phase-Contrast Microscopy

Exercise 4 Fluorescence Microscopy

Exercise 5 Microscopic Measurements

Part 2 Survey of Microorganisms

Exercise 6 Protozoans, Algae, and Cyanobacteria

Exercise 7 Ubiquity of Bacteria

Exercise 8 The Fungi: Yeasts and Molds

Part 3 Manipulation of Microorganisms

Exercise 9 Aseptic Technique

Exercise 10 Pure Culture Techniques

Part 4 Staining and Observation of Microorganisms

Exercise 11 Smear Preparation

Exercise 12 Simple Staining

Exercise 13 Negative Staining

Exercise 14 Capsular Staining

Exercise 15 Gram Staining

Exercise 16 Spore Staining: Two Methods

Exercise 17 Acid-Fast Staining: Ziehl-Neelsen Method

Exercise 18 Motility Determination

Part 5 Culture Methods

Exercise 19 Culture Media Preparation

Exercise 20 Preparation of Stock Cultures

Exercise 21 Cultivation of Anaerobes

Exercise 22 Enumeration of Bacteria: The Standard Plate Count

Exercise 23 Slime Mold Culture

Exercise 24 Slide Culture: Molds

Part 6 Bacterial Viruses

Exercise 25 Determination of a Bacteriophage Titer

Exercise 26 Burst Size Determination: A One-Step Growth Curve

Exercise 27 Isolation of Phage from Flies

Exercise 28 Phage Typing

Part 7 Enviornmental Influences and Control of Microbial Growth

Exercise 29 Temperature: Effects on Growth

Exercise 30 Temperature: Lethal Effects

Exercise 31 pH and Microbial Growth

Exercise 32 Water Activity and Osmotic Pressure

Exercise 33 Ultraviolet Light: Lethal Effects

Exercise 34 The Effects of Lysozyme on Bacterial Cells

Exercise 35 Evaluation of Alcohol: Its Effectiveness as an Antiseptic

Exercise 36 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method

Exercise 37 Evaluation of Antiseptics: The Filter Paper Disk Method

Exercise 38 Effectiveness of Hand Scrubbing

Part 8 Identification of Unknown Bacteria

Exercise 39 Morphological Study of Unknown Bacterium

Exercise 40 Cultural Characteristics

Exercise 41 Physiological Characteristics: Oxidation and Fermentation Tests

Exercise 42 Physiological Characteristics: Hydrolytic and Degradative Reactions

Exercise 43 Physiological Characteristics: Multiple Test Media

Exercise 44 Use of Bergey's Manual

Part 9 Miniaturized Multitest Systems

Exercise 45 Enterobacteriaceae Identification: The API 20E System

Exercise 46 Enterobacteriaceae Identification: The Enterotube II System

Exercise 47 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System

Exercise 48 Staphyococcus Identification: The API Staph-Ident System

Part 10 Diversity and Environmental Microbiology

Exercise 49 Isolation of an Antibiotic Producer: The Actinomyces

Exercise 50 Nitrogen Cycle: Ammonification

Exercise 51 Symbiotic Nitrogen Fixation: Rhizobium

Exercise 52 Free-Living Nitrogen Fixation: Azotobacter

Exercise 53 Denitrification: Paracoccus Denitrificans

Exercise 54 The Winogradsky Column

Exercise 55 Purple Nonsulfer Photosynthetic Bacteria

Exercise 56 Sulfate Reducing Bacteria: Desulfovibrio

Exercise 57 Bacterial Commensalism

Exercise 58 Bacterial Synergism

Exercise 59 Microbial Antagonism

Part 11 Applied Microbiology

Exercise 60 Bacterial Counts of Foods

Exercise 61 Bacteriological Examination of Water: Qualitative Tests

Exercise 62 The Membrane Filter Method

Exercise 63 Reductase Test

Exercise 64 Microbial Spoilage of Canned Food

Exercise 65 Microbiology of Alcohol Fermentation

Part 12 Bacterial Genetics and Biotechnology

Exercise 66 Mutant Isolation by Replica Plating

Exercise 67 Bacterial Transformation

Exercise 68 Polymerase Chain Reaction for Amplifying DNA

Exercise 69 Plasmid Isolation

Part 13 Medical Microbiology and Immunology

Exercise 70 The Staphylococci: Isolation and Identification

Exercise 71 The Streptococci: Isolation and Identification

Exercise 72 Gram-Negative Intestinal Pathogens

Exercise 73 Slide Agglutination Test: Serological Typing

Exercise 74 Slide Agglutination (Latex) Test: For S. aureus Identification

Exercise 75 Tube Agglutination Test: The Heterophile Antibody Test

Exercise 76 White Blood Cell Study: The Differential WBC Count

Exercise 77 Blood Grouping

Exercise 78 A Synthetic Epidemic

Appendix A Tables

Appendix B Indicators, Stains, Reagents

Appendix C Media

Appendix D Identification Charts

Appendix E The Streptococci: Classification, Habitat, Pathology, and Biochemical Characteristics