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Preface | p. xi |
List of Contributors | p. xiii |
Steps in Biofilm Sampling and Characterization in Biofouling Cases | p. 1 |
Introduction | p. 1 |
Sampling Strategy | p. 2 |
Field Methods In Situ | p. 3 |
Laboratory Methods | p. 4 |
Laboratory Methods to Examine Removed Biofilms | p. 10 |
Identification | p. 11 |
Chemical and Biochemical Analysis | p. 13 |
Genetic Methods | p. 16 |
Immunological Methods | p. 17 |
Conclusions and Outlook | p. 17 |
References | p. 18 |
Rotating Annular Reactors for Controlled Growth of Biofilms | p. 23 |
Introduction | p. 23 |
Material and Methods | p. 25 |
Results | p. 29 |
Discussion | p. 34 |
References | p. 39 |
Microcalorimetry for Evaluating Countermeasures Against Biofouling in Water Circulation Systems | p. 41 |
Introduction | p. 41 |
Materials and Methods | p. 42 |
Results and Discussion | p. 44 |
Conclusions | p. 48 |
Acknowledgement | p. 49 |
References | p. 49 |
Extraction and Quantification of Extracellular Polymeric Substances From Wastewater | p. 51 |
Introduction | p. 51 |
Extraction Methods | p. 53 |
Quantification | p. 57 |
Cell Lysis | p. 59 |
Applications | p. 60 |
Acknowledgements | p. 64 |
Nomenclature | p. 64 |
References | p. 65 |
Cell Biomass Determination in Sewer Biofilms and a Monospecies Pseudomonas Putida Biofilm | p. 69 |
Introduction | p. 69 |
Direct Determination | p. 71 |
Indirect Methods | p. 77 |
Conclusions | p. 80 |
References | p. 81 |
Simultaneous Morphological and Population Analyses of Environmental Biofilms | p. 83 |
Principle of the Method | p. 83 |
Range of Applications | p. 84 |
Methods | p. 85 |
Results | p. 94 |
Comparison with Other Methods | p. 98 |
Discussion | p. 100 |
Nomenclature | p. 103 |
Acknowledgements | p. 104 |
References | p. 104 |
Development of a New Method to Determine the Metabolic Potential of Bacteria in Drinking Water Biofilms: Probe Active Counts (Pac) | p. 107 |
Introduction | p. 107 |
Materials and Methods | p. 108 |
Results and Discussion | p. 111 |
References | p. 119 |
Formation and Bacterial Composition of Young, Natural Biofilms Obtained From Public Bank-Filtered Drinking Water Systems | p. 123 |
Introduction | p. 123 |
Materials and Methods | p. 124 |
Results | p. 128 |
Discussion | p. 136 |
Acknowledgements | p. 140 |
References | p. 140 |
The Use of Immunological Techniques and Scanning Confocal Laser Microscopy for the Characterization of Agrobacterium Tumefaciens and Pseudomonas Fluorescens, Atrazine-Utilizing Biofilms | p. 143 |
Introduction | p. 143 |
Materials and Methods | p. 144 |
Results | p. 147 |
Discussion | p. 151 |
Acknowledgements | p. 152 |
References | p. 152 |
The in Situ Detection of a Microbial Biofilm Community on Karst Rock Coupon in a Groundwater Habitat | p. 155 |
Introduction | p. 155 |
Materials and Methods | p. 156 |
Results and Discussion | p. 157 |
Summary | p. 161 |
References | p. 162 |
Investigation of Spatial and Temporal Gradients in Fixed-Bed Biofilm Reactors for Wastewater Treament | p. 165 |
Introduction | p. 165 |
Aim of the Study | p. 166 |
Experimental Setup | p. 167 |
Chlorophenol Elimination by Biofilms Under Different Operating Conditions | p. 168 |
Quantitative Determination of Biomass and Biological Activity | p. 171 |
Investigation of the Bacterial Community of the Biofilm | p. 178 |
The Protozoa and Metazoa in Biofilms | p. 183 |
Conclusions | p. 188 |
Nomenclature | p. 190 |
Acknowledgements | p. 191 |
References | p. 191 |
Quantitative Microscopy in Biofilm Studies | p. 195 |
Introduction | p. 195 |
Materials and Methods | p. 195 |
Results | p. 198 |
Discussion | p. 208 |
Acknowledgements | p. 208 |
References | p. 208 |
Confocal Laser Scanning Microscopy (Clsm) of Biofilms | p. 211 |
Introduction | p. 211 |
Clsm History | p. 212 |
Confocal Imaging | p. 213 |
Advantages/Disadvantages | p. 213 |
Techniques | p. 214 |
Applications | p. 217 |
Conclusions | p. 219 |
Acknowledgement | p. 219 |
References | p. 219 |
Microelectrodes and Tube Reactors in Biofilm Research | p. 225 |
Introduction | p. 225 |
Materials and Methods | p. 226 |
Results | p. 231 |
Discussion | p. 238 |
References | p. 239 |
Index | p. 241 |
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The Used, Rental and eBook copies of this book are not guaranteed to include any supplemental materials. Typically, only the book itself is included. This is true even if the title states it includes any access cards, study guides, lab manuals, CDs, etc.