Bioseparations Science and Engineering

by Harrison, Roger G.; Todd, Paul W.; Rudge, Scott R.; Petrides, Demetri

• ISBN13: 9780195123401
• ISBN10: 0195123409
• Format: Hardcover
• Copyright: 2002-10-31
• Publisher: Oxford University Press

Summary

Designed for undergraduates, graduate students, and industry practitioners, Bioseparations Science and Engineering fills a critical need in the field. Current, comprehensive, and concise, it covers bioseparations unit operations in greater depth than other texts on this topic. In each of the chapters, the authors use a consistent method of explaining unit operations, starting with a qualitative description noting the significance and general application of the unit operation. They then illustrate the scientific application of the operation, develop the required mathematical theory, and finally, describe the applications of the theory in engineering practice, with an emphasis on design and scaleup. Unique to this text is a chapter dedicated to bioseparations process design and economics, in which a process simular, SuperPro DesignerRG is used to analyze and evaluate the production of three important biological products. Other unique features include basic information about bioproducts and engineering analysis and a chapter with bioseparations laboratory exercises. Bioseparations Science and Engineering is ideal for students and professionals alike. Features Incorporates numerous example problems within the chapters Offers extensive sets of problems at the end of chapters Includes basic information about bioproducts Provides thorough coverage of analytical methods for bioproducts Uses the simulation software SuperPro DesignerRG to illustrate the analysis and evaluation of the production of citric acid, recombinant human insulin, and monoclonal antibodies Includes laboratory exercises that support text material Accompanied by a solutions manual available to instructors who adopt this text Supplemented by a website (www.biosep.ou.edu) with new problems and examples and links to useful databases and manufacturers of bioseparations equipment and supplies

Author Biography

Roger G. Harrison is an Associate Professor of Chemical Engineering at the University of Oklahoma.

Table of Contents

Preface

All chapters (except Chapter 12) end with the following sections: Summary, Nomenclature, Problems, and References

Preface

Introduction to Bioproducts and Bioseparations

Instructional Objectives

Broad Classification of Bioproducts

Small Biomolecules

Primary Metabolites

Secondary Metabolites

Summary of Small Biomolecules

Macromolecules: Proteins

Primary Structure

Secondary Structure

Tertiary Structure

Example

Effect of a Reducing Agent on Protein Structure and Mobility

Quaternary Structure

Prosthetic Groups and Hybrid Molecules

Functions and Commercial Uses of Proteins

Stability of Proteins

Recombinant Protein Expression

Macromolecules: Nucleic Acids and Oligonucleotides

Macromolecules: Polysaccharides

Particulate Products

Introduction to Bioseparations: Engineering Analysis

Stages of Downstream Processing

Example

:Initial Selection of Purification Steps

Basic Principles of Engineering Analysis

Process and Product Quality

Criteria for Process Development

The Route to Market

The Chemical and Applications Range of the Bioproduct

Documentation of Pharmaceutical Bioproducts

GLP and cGMP

Formulation

Analytical Methods

Instructional Objectives

Specifications

Assay Attributes

Precision

Accuracy

Specificity

Linearity, Limit of Detection, and Limit of Quantitation

Range

Robustness

Analysis of Biological Activity

Animal Model Assays

Cell-Line-Derived Bioassays

In Vitro Biochemical Assays

Example

:Coupled Enzyme Assay for Alcohol Oxidase

Analysis of Purity

Electrophoretic Analysis

Example

:Estimation of the Maximum Temperature in an Electrophoresis Gel

High Performance Liquid Chromatography (HPLC)

Mass Spectrometry

Coupling of HPLC with Mass Spectrometry

UV Absorbance

Example

:Determination of Molar Absorptivity

CHNO/Amino Acid Analysis (AAA)

Example

:Calculations Based on CHNO Analysis

Protein Assays

Enzyme-Linked Immunosorbent Assay

Gas Chromatography

DNA Hybridization

ICP/MS (AA)

Dry Weight

Microbiology Assays

Sterility

Bioburden

Endotoxin

Virus and Phage

Cell Lysis and Flocculation

Instructional Objectives

Some Elements of Cell Structure

Prokaryotic Cells

Eukaryotic Cells

Cell Lysis

Osmotic and Chemical Cell Lysis

Mechanical Methods of Lysis

Flocculation

The Electric Double Layer

Example

:Dependence of the Debye Radius on the Type of Electrolyte

Forces between Particles and Flocculation by Electrolytes

Example

:Sensitivity of Critical Flocculation Concentration to Temperature and Counter-Ion Charge Number

The Schulze-Hardy Rule

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