Calcium Binding Proteins

by Permyakov, Eugene; Kretsinger, Robert H.

• ISBN13: 9780470525845
• ISBN10: 0470525843
• Edition: 1st
• Format: Hardcover
• Copyright: 2011-01-11
• Publisher: Wiley

Summary

Calcium Binding Proteins explains the unique and highly diverse functions of calcium in biology, which are realized by calcium binding proteins. The structures and physical characteristics of these calcium binding proteins are described, as well as their functions and general patterns of their evolution. Techniques that underlie the description of proteins are discussed, including NMR, circular dichroism, optical rotatory dispersion spectroscopy, calorimetry,and crystallography. The book discusses the patterns of bochmical phenomena such as calcium homeostasis, mineralization, and cell signaling that involve specific proteins. It summarizes ongoing research and presents general hypotheses that help to focus future research, and also provides a conceptual framework and a description of the underlying techniques that permits someone entering the field to become conversant.

Author Biography

Eugene A. Permyakov received his PhD in physics and mathematics at the Moscow Institute of Physics and Technology in 1976 and defended his Doctor of Science dissertation in biology at Moscow State University in 1989. From 1970 to 1994, he worked at the Institute of Theoretical and Experimental Bio-physics of the Russian Academy of Sciences. Since 1994 he has been the Director of the Institute for Biological Instrumentation of the Russian Academy of Sciences. He is known for his work on metal binding proteins and the intrinsic fluorescence method. His primary research focus is the study of physico-chemical and functional properties of metal binding (especially calcium-binding) proteins. Robert H. Kretsinger received his PhD in biophysics at the Massachusetts Institute of Technology under the supervision of Alex Rich in 1964. In 1967 he set up a protein crystallography lab in the Department of Biology at the University of Virginia, where he presently teaches, and determined the crystal structure of parvalbumin as well as describing the EF-hand domain in 1971. He has subsequently investigated the structures, functions, and evolution of various EF-hand homologs, along with other protein families. From 1978 to 1995, he directed a national facility to build multiwire area detectors for x-ray diffraction and to collect data from protein crystals.

Table of Contents

Historical Perspective

Physiological Processes Involving Calcium Binding Proteins

Comparison of the Ca²⁺ Ion with Other Metal Cations

Complexes of Calcium and Other Cations with Low Molecular Weight

Stoichiometry, Kinetics, and Thermodynamics of Calcium Binding

Experimental Methods Used to Study Calcium Binding to Proteins

Structure and Evolution of Proteins

Protein Complexes with Metals, other than Calcium

Non-essential Metals

Parvalbumin

EF-hand Proteins

Cytosolic, Calcium Binding Proteins, Lacking EF-hands

Extracellular Calcium Binding Proteins

Interactions of Calcium Binding Proteins with Other Metal Ions

Interactions of Calcium Binding Proteins with Other Proteins and with Membranes

Mutagenesis and Control of Gene Expression

Table of Contents provided by Publisher. All Rights Reserved.