Cell Migration

by Guan, Jun-Lin

• ISBN13: 9781588293824
• ISBN10: 1588293823
• Format: Hardcover
• Copyright: 2004-11-15
• Publisher: Humana Pr Inc

Summary

A collection of classic, novel, and state-of-the-art methods for the study of cell migration in cultured cells, different model organisms, and specialized cells in normal development and disease. Highlights include basic assays that apply to all cell migration studies in vitro, assays in various model organisms, and assays for cancer cells, endothelial cells, and neurons both in vitro and in animal models. The protocols follow the successful Methods in Molecular Biology'„¢ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Table of Contents

Preface	v
Contributors	xiii
Part I. Introduction
Cell Migration: An Overview	3	(12)
Donna J. Webb
Huaye Zhang
Alan F. Horwitz
Part II. Basic Cell Migration and Related Assays
Boyden Chamber Assay	15	(8)
Hong-Chen Chen
Wound-Healing Assay	23	(8)
Luis G. Rodriguez
Xiaoyang Wu
Jun-Lin Guan
Analysis of Cell Migration Using the Dunn Chemotaxis Chamber and Time-Lapse Microscopy	31	(12)
Claire M. Wells
Anne J. Ridley
Cell-Adhesion Assays	43	(12)
Dennis F. Kucik
Chuanyue Wu
Cell-Spreading Assays	55	(14)
Allison L. Berrier
Susan E. LaFlamme
Cell-Scatter Assay	69	(10)
Hong-Chen Chen
Cell Migration Analyses Within Fibroblast-Derived 3-D Matrices	79	(18)
Edna Cukierman
Part III. Analysis of Cell Migration in Specialized Cell Systems
Tumor Cell Invasion Assays	97	(10)
Leslie M. Shaw
Analysis of Endothelial Cell Migration Under Flow	107	(16)
Song Li
Angiogenesis Assays in the Chick CAM	123	(14)
Chris Storgard
David Mikolon
Dwayne G. Stupack
Investigations of Neuronal Migration in the Central Nervous System	137	(22)
Michael E. Ward
Yi Rao
Part IV. Analysis of Cell Migration in Model Organisms
Analysis of Cell Migration in Caenorhabditis elegans	159	(16)
M. Afaq Shakir
Erik A. Lundquist
Analysis of Cell Migration Using Drosophila as a Model System	175	(28)
Jocelyn A. McDonald
Denise J. Montell
Imaging Cell Movement During Dorsal Closure in Drosophila Embryos	203	(8)
William Wood
Antonio Jacinto
Analysis of Cell Movements in Zebrafish Embryos: Morphometrics and Measuring Movement of Labeled Cell Populations In Vivo	211	(24)
Diane S. Sepich
Lilianna Solnica-Krezel
The Xenopus Embryo as a Model System for Studies of Cell Migration	235	(12)
Douglas W. DeSimone
Lance Davidson
Mungo Marsden
Dominique Alfandari
Neural Crest Migration Methods in the Chicken Embryo	247	(22)
Maria Elena de Bellard
Marianne Bronner-Fraser
In Vivo and In Vitro Models of Mammalian Angiogenesis	269	(20)
Mien V. Hoang
Donald R. Senger
Part V. Biochemical and Novel Approaches in Cell Migration
Measurement of Protein Tyrosine Phosphorylation in Cell Adhesion	289	(14)
Vidhya V. Iyer
Christoph Ballestrem
Joachim Kirchner
Benjamin Geiger
Michael D. Schaller
Cell-Adhesion Assays: Fabrication of an E-Cadherin Substratum and Isolation of Lateral and Basal Membrane Patches	303	(18)
Frauke Drees
Amy Reilein
W. James Nelson
Application of Microscope-Based FRET to Study Molecular Interactions in Focal Adhesions of Live Cells	321	(14)
Christoph Ballestrem
Benjamin Geiger
Use of Multiphoton Imaging for Studying Cell Migration in the Mouse	335	(12)
Andrea Flesken-Nikitin
Rebecca M. Williams
Warren R. Zipfel
Watt W. Webb
Alexander Yu. Nikitin
Using Microfluidic Channel Networks to Generate Gradients for Studying Cell Migration	347	(12)
Daniel S. Rhoads
Sharvari M. Nadkarni
Loling Song
Camilla Voeltz
Eberhard Bodenschatz
Jun-Lin Guan
Index	359