Cellular Programming and Reprogramming

by Ding, Sheng

• ISBN13: 9781607616900
• ISBN10: 1607616904
• Edition: 1st
• Format: Hardcover
• Copyright: 2010-04-13
• Publisher: Humana Pr Inc

Summary

Before the therapeutic potential of cell replacement therapy or the development of therapeutic drugs for stimulating the body’s own regenerative ability to repair cells damaged by disease and injury can be fully realized, control of stem cell fate, immuno-rejection, and limited cell sources must be overcome. In Cellular Programming and Reprogramming: Methods and Protocols, expert researchers cover the most recent technologies and their related mechanisms involved in the programming and reprogramming of cell fate. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and notes to highlight tips on troubleshooting and avoiding known pitfalls.Essential and cutting-edge, Cellular Programming and Reprogramming: Methods and Protocols promises to aid scientists attempting to advance stem cell biology in order to better treat devastating human diseases, including cardiovascular disease, neurodegenerative disease, musculoskeletal disease, diabetes, and cancer.

Table of Contents

Preface	p. v
Contributors	p. ix
Human Embryonic Stem Cell Derivation, Maintenance, and Differentiation to Trophoblast	p. 1
Isolation and Maintenance of Mouse Epiblast Stem Cells	p. 25
Functional Assays for Hematopoietic Stem Cell Self-Renewal	p. 45
Isolation Procedure and Characterization of Multipotent Adult Progenitor Cells from Rat Bone Marrow	p. 55
Generation of Functional Insulin-Producing Cells from Human Embryonic Stem Cells In Vitro	p. 79
Mesoderm Cell Development from ES Cells	p. 87
Directed Differentiation of Red Blood Cells from Human Embryonic Stem Cells	p. 105
Directed Differentiation of Neural-stem cells and Subtype-Specific Neurons from hESCs	p. 123
Directing Human Embryonic Stem Cells to a Retinal Fate	p. 139
Bovine Somatic Cell Nuclear Transfer	p. 155
Cell Fusion-Induced Reprogramming	p. 179
An Improved Method for Generating and Identifying Human Induced Pluripotent Stem Cells	p. 191
Using Small Molecules to Improve Generation of Induced Pluripotent Stem Cells from Somatic Cells	p. 207
Reprogramming of Committed Lymphoid Cells by Enforced Transcription Factor Expression	p. 219
Reprogramming of B Cells	p. 233
Adult Cell Fate Reprogramming: Converting Liver to Pancreas	p. 251
In Vitro Reprogramming of Pancreatic Cells to Hepatocytes	p. 285
Generation of Novel Rat and Human Pluripotent Stem Cells by Reprogramming and Chemical Approaches	p. 293
Small Molecule Screen in Zebrafish and HSC Expansion	p. 301
Zebrafish Small Molecule Screen in Reprogramming/Cell Fate Modulation	p. 317
Index	p. 329
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