Chloroplast Research in Arabidopsis

by Jarvis, R. Paul

• ISBN13: 9781617792366
• ISBN10: 1617792365
• Format: Hardcover
• Copyright: 2011-08-23
• Publisher: Humana Pr Inc

Summary

Chloroplast biology is essential for the survival and flourishing of life on Earth; however, over the years, it has been studied in a variety of different organisms, leading to the significant disadvantage that findings which were made by using different experimental systems or species were not always directly cross-comparable.#xC2;#xA0; The relatively recent adoption of Arabidopsis thaliana as the model organism of choice for plant science research, across the globe, has led to its emergence as a pre-eminent system for research on chloroplasts and other types of plastid.#xC2;#xA0; In Chloroplast Research in Arabidopsis: Methods and Protocols, expert researchers bring together some of the most important, modern techniques and approaches for chloroplast research, with the unifying theme of Arabidopsis as the model system. Volume II explores topics such as multiprotein complexes, protein-protein interactions, omics and large-scale analyses, proteomics and suborganellar fractionation, as well as photosynthesis and biochemical analysis.#xC2;#xA0; Written in the highly successful Methods in Molecular Biology#xE2;#x1E;#xA2; series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.#xC2;#xA0;Authoritative and convenient, Chloroplast Research in Arabidopsis: Methods and Protocols serves as an ideal reference for all researchers with a general interest in chloroplasts, plastids, or related processes.

Author Biography

Authoritative and convenient, Chloroplast Research in Arabidopsis Methods and Protocols serves as an ideal reference for all researchers with a general interest in chloroplasts plastids, or related processes.

Table of Contents

preface	p. v
Contributors	p. ix
Multiprotein Complexes and Protein-Protein Interactions
One- and Two-Dimensional Blue Native-PAGE and Immunodetection of Low-Abundance Chloroplast Membrane Protein Complexes	p. 3
Analysis of Thylakoid Protein Complexes by Two-Dimensional Electrophoretic Systems	p. 19
Preparation of Multiprotein Complexes from Arabidopsis Chloroplasts Using Tandem Affinity Purification	p. 31
Studying Interactions Between Chloroplast Proteins in Intact Plant Cells Using Bimolecular Fluorescence Complementation and Förster Resonance Energy Transfer	p. 31
Studying Chloroplast Protein Interactions In Vitro: An Overview of the Available Methods	p. 67
Omics and Large-Scale Analyses
Proteome Databases and Other Online Resources for Chloroplast Research in Arabidopsis	p. 93
Use of Transcriptomics to Analyze Chloroplast Processes in Arabidopsis	p. 117
Use of Non-aqueous Fractionation and Metabolomics to Study Chloroplast Function in Arabidopsis	p. 135
Chloroplast Phenomics: Systematic Phenotypic Screening of Chloroplast Protein Mutants in Arabidopsis	p. 189
Proteomics and Suborganellar Fractionation
Preparation of Envelope Membrane Fractions from Arabidopsis Chloroplasts for Proteomic Analysis and Other Studies	p. 189
Preparation of Stroma, Thylakoid Membrane, and Lumen Fractions from Arabidopsis thaliana Chloroplasts for Proteomic Analysis	p. 207
Preparation of Plastoglobules from Arabidopsis Plastids for Proteomic Analysis and Other Studies	p. 223
Preparation and Proteomic Analysis of Chloroplast Ribosomes	p. 241
The Workflow for Quantitative Proteome Analysis of Chloroplast Development and Differentiation, Chloroplast Mutants, and Protein Interactions by Spectral Counting	p. 265
Use of Phosphoproteomics to Study Posttranslational Protein Modifications in Arabidopsis Chloroplasts	p. 283
Photosynthesis and Biochemical Analysis
Use of a Pulse-Amplitude Modulated Chlorophyll Fluorometer to Study the Efficiency of Photosynthesis in Arabidopsis Plants	p. 299
Gas Exchange Measurements for the Determination of Photosynthetic Efficiency in Arabidopsis Leaves	p. 311
Measurement of the ¿pH and Electric Field Developed Across Arabidopsis Thylakoids in the Light	p. 327
Measurement of Chloroplast ATP Synthesis Activity in Arabidopsis	p. 343
Methods for Analysis of Photosynthetic Pigments and Steady-State Levels of Intermediates of Tetrapyrrole Biosynthesis	p. 357
Analysis of Starch Metabolism in Chloroplasts	p. 387
Analysis of Lipid Content and Quality in Arabidopsis Plastids	p. 411
Index	p. 427
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