Clinical Applications of Mass Spectrometry

by Garg, Uttam; Hammett-stabler, Catherine A.

• ISBN13: 9781607614586
• ISBN10: 1607614588
• Format: Hardcover
• Copyright: 2010-01-03
• Publisher: Humana Pr Inc

Summary

As mass spectrometric methods now offer a level of specificity and sensitivity unrealized by spectrophotometric- and immunoassay-based methods, mass spectrometry has entered the clinical laboratory where it is being used for a wide range of applications. In Clinical Applications of Mass Spectrometry: Methods and Protocols, expert researchers provide detailed step-by-step procedures for the analysis of number of analytes of clinical importance. This versatile and expansive volume covers mass spectrometry methods for analytes including a variety of drugs, hormones, and metabolic compounds spanning the disciplines of toxicology, therapeutic drug monitoring, endocrinology, and pediatric metabolism. Written in the highly successful Methods in Molecular Biology'„¢ series format, chapters include brief introductions to the analytes, lists of the necessary materials and reagents, readily reproducible analytical protocols, and detailed notes on troubleshooting and avoiding known pitfalls.Comprehensive and dependable, Clinical Applications of Mass Spectrometry: Methods and Protocols offers its readers a wide array of valuable methods for experienced mass spectrometric labs that are looking to introduce new analyses as well as for those laboratories currently considering the addition of this resourceful and vital technology.

Table of Contents

Preface	p. vii
Contributors	p. xiii
The Evolution of Mass Spectrometry in the Clinical Laboratory	p. 1
Measurement of Plasma/Serum Acylcarnitines Using Tandem Mass Spectrometry	p. 9
Comprehensive Determination of Amino Acids for Diagnosis of Inborn Errors of Metabolism	p. 27
Identification and Quantitation of Amphetamine, Methamphetamine, MDMA, Pseudoephedrine, and Ephedrine in Blood, Plasma, and Serum Using Gas Chromatography-Mass Spectrometry (GC/MS)	p. 37
Quantification of Antidepressants Using Gas Chromatography-Mass Spectrometry	p. 45
Quantitation of Argatroban in Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (UPLC-ESI-MS/MS)	p. 57
Quantitation of Amobarbital, Butalbital, Pentobarbital, Phenobarbital, and Secobarbital in Urine, Serum, and Plasma Using Gas Chromatography-Mass Spectrometry (GC-MS)	p. 65
Quantitation of Benzodiazepines in Blood and Urine Using Gas Chromatography-Mass Spectrometry (GC-MS)	p. 75
LC-MS/MS Analysis of 13 Benzodiazepines and Metabolites in Urine, Serum, Plasma, and Meconium	p. 89
Simultaneous Determination and Quantification of 12 Benzodiazepines in Serum or Whole Blood Using UPLC/MS/MS	p. 107
Determination of Benzoic Acid in Serum or Plasma by Gas Chromatography-Mass Spectrometry (GC/MS)	p. 121
Quantification of Busulfan in Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (HPLC-ESI-MS/MS)	p. 129
Quantitation of Total ll-Nor-9-Carboxy-Delta 9-Tetrahydrocannabinol in Urine and Blood Using Gas Chromatography-Mass Spectrometry (GC-MS)	p. 137
Quantitation of Cocaine, Benzoylecgonine, Ecgonine Methyl Ester, and Cocaethylene in Urine and Blood Using Gas Chromatography-Mass Spectrometry (GC-MS)	p. 145
Identification and Quantitation of Cocaine, Benzoylecgonine, and Cocaethylene in Blood, Serum, and Plasma Using Ultra-Performance liquid Chromatography Coupled to Tandem Mass Spectrometry (UPLC-MS/MS)	p. 157
Detection and Quantification of Cocaine and Benzoylecgonine in Meconium Using Solid Phase Extraction and UPLC/MS/MS	p. 165
Diagnosis of Creatine Metabolism Disorders by Determining Creatine and Guanidinoacetate in Plasma and Urine	p. 175
Broad Spectrum Drug Screening Using Electron-Ionization Gas Chromatography-Mass Spectrometry (EI-GCMS)	p. 187
Broad Spectrum Drug Screening Using Liquid Chromatography-Hybrid Triple Quadrupole Linear Ion Trap Mass Spectrometry	p. 203
High Sensitivity Measurement of Estrone and Estradiol in Serum and Plasma Using LC-MS/MS	p. 219
3-Hydroxy-Fatty Acid Analysis by Gas Chromatography-Mass Spectrometry	p. 229
Quantitation of Fentanyl in Blood and Urine Using Gas Chromatography-Mass Spectrometry (GC-MS)	p. 245
Determination of Total Homocysteine in Plasma Using Liquid Chromatography Coupled to Tandem Mass Spectrometry (LC/MS/MS)	p. 253
Quantitation of Homovanillic Acid (HVA) and Vanillylmandelic Acid (VMA) in Urine Using Gas Chromatography-Mass Spectrometry (GC/MS)	p. 261
Quantitation of 17-OH-Progesterone (OHPG) for Diagnosis of Congenital Adrenal Hyperplasia (CAH)	p. 271
Determination of Hydroxyurea in Serum or Plasma Using Gas Chromatography-Mass Spectrometry (GC-MS)	p. 279
Quantitation of Ibuprofen in Blood Using Gas Chromatography-Mass Spectrometry (GC-MS)	p. 289
Quantitation of Indomethacin in Serum and Plasma Using Gas Chromatography-Mass Spectrometry (GC-MS)	p. 297
Quantitation of Iothalamate in Urine and Plasma Using Liquid Chromatography Electrospray Tandem Mass Spectrometry (HPLC-ESI-MS/MS)	p. 307
Identification and Quantitation of Ketamine in Biological Matrices Using Gas Chromatography-Mass Spectrometry (GC-MS)	p. 317
Measurement of Filter Paper Bloodspot Lead by Inductively Coupled Plasma-Mass Spectrometry (ICP-MS)	p. 327
Multiplex Lysosomal Enzyme Activity Assay on Dried Blood Spots Using Tandem Mass Spectrometry	p. 339
Gas Chromatography-Mass Spectrometry Method for the Determination of Methadone and 2-Ethylidene-l,5-Dimethyl-3,3-DiphenylpyrroIidine (EDDP)	p. 351
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS-MS) Method for Monitoring Methotrexate in the Setting of Carboxypeptidase-G2 Therapy	p. 359
Quantitation of Methylmalonic Acid in Serum or Plasma Using Isotope Dilution-Selected Ion Gas Chromatography-Mass Spectrometry	p. 365
Quantitation of Methylmalonic Acid in Plasma Using Liquid Chromatography - Tandem Mass Spectrometry	p. 371
Monitoring of Mycophenolate Acid in Serum or Plasma Using LC Tandem Mass Spectrometry	p. 379
Quantitation of Nicotine, Its Metabolites, and Other Related Alkaloids in Urine, Serum, and Plasma Using LC-MS-MS	p. 389
Quantitation of Opioids in Blood and Urine Using Gas Chromatography-Mass Spectrometry (GC-MS)	p. 399
Quantitation of Morphine, Codeine, Hydrocodone, Hydromorphone, Oxycodone, Oxymorphone, and 6-Monoacetylmorphine (6-MAM) in Urine, Blood, Serum, or Plasma Using Liquid Chromatography with Tandem Mass Spectrometry Detection	p. 411
Urine Organic Acid Analysis for Inherited Metabolic Disease Using Gas Chromatography-Mass Spectrometry	p. 423
Identification of Urine Organic Acids for the Detection of Inborn Errors of Metabolism Using Urease and Gas Chromatography-Mass Spectrometry (GC-MS)	p. 433
Quantitation of Orotic Acid in Urine Using Isotope Dilution-Selected Ion Gas Chromatography-Mass Spectrometry	p. 445
Quantitation of Oxycodone in Blood and Urine Using Gas Chromatography-Mass Spectrometry (GC-MS)	p. 453
Quantitation of Phencyclidine (PCP) in Urine and Blood Using Gas Chromatography-Mass Spectrometry (GC-MS)	p. 461
Quantitation of Sirolimus Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS-MS)	p. 469
Quantitation of Tacrolimus in Whole Blood Using High Performance Liquid Chromatography-Tandem Mass Spectrometry (HPLC-MS-MS)	p. 479
Analysis of Testosterone in Serum Using Mass Spectrometry	p. 489
Determination of Tetrahydrozoline m Urine and Blood Using Gas Chromatography-Mass Spectrometry (GC-MS)	p. 501
Quantitation of 25-OH-Vitamin D (250HD) Using Liquid Tandem Mass Spectrometry (LC-MS-MS)	p. 509
Identification and Quantitation of Zolpidem in Biological Matrices Using Gas Chromatography-Mass Spectrometry (GC-MS)	p. 519
Identification and Quantitation of Zopiclone in Biological Matrices Using Gas Chromatography-Mass Spectrometry (GC-MS)	p. 527
Subject Index	p. 535
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