DNA Transfer to Cultured Cells

by Ravid, Katya; Freshney, R. Ian

• ISBN13: 9780471165729
• ISBN10: 0471165727
• Edition: 1st
• Format: Paperback
• Copyright: 1998-08-14
• Publisher: Wiley-Liss

Summary

DNA transfer to cultured cells Edited by Katya Ravid and R. Ian Freshney Rapid advances in DNA transfer technology have transformed many disciplines, ranging from molecular genetics to biotechnology. Scientists now have the means to introduce copies of genes into different cell types, then detect the expression of these genes in the cell. It is now possible to regulate cell growth that may lead to cancer, develop new biopharmaceuticals, and apply knowledge about the role of genes in cell processes to basic research in molecular genetics. DNA Transfer to Cultured Cells is the first quick reference to all of the established techniques for the transfer of genetic material to cells in vitro. Featuring contributions by leading researchers in the field, this detailed guide walks the reader through a variety of DNA transfer methods, describes their application to specific cell types, and integrates aspects of molecular biology with tissue culture. Offering overviews and detailed protocols for the techniques under discussion in each of its sections, this book covers an exceptionally broad array of topics, including: * Viral infection * Electroporation * Phosphate precipitation * DEAE Dextran * Liposomes * Yeast artificial chromosomes (YACs) * Whole chromosome transfer * Enhanced expression. Special sections at the end of each chapter list suppliers for necessary reagents and materials. This easy-to-use, self-contained guide addresses key developments of recent years as well as emerging trends in DNA transfer. For practical applications in cell biology, genetics, heredity, biotechnology, or evolution, DNA Transfer to Cultured Cells is a unique and unparalleled resource.

Author Biography

Katya Ravid is the editor of DNA Transfer to Cultured Cells, published by Wiley. R. IAN FRESHNEY, PhD, is an honorary senior research fellow in the Centre for Oncology and Applied Pharmacology, part of the Cancer Research UK Beatson Laboratories at the University of Glasgow. He is the author or editor of numerous books and a world-renowned expert on cell culture techniques.

Table of Contents

Preface	vii	(2)
Contributors	ix	(4)
List of Abbreviations	xiii
Chapter 1. Production and Use of Retroviruses	1	(26)
Geoffrey G. Hicks
Jin Chen
H. Earl Ruley
Chapter 2. Baculovirus Expression and the Study of the Regulation of the Tumor Suppressor Protein p53	27	(28)
Carol A. Midgley
Ashley L. Craig
Jeffery P. Hite
Ted R. Hupp
Chapter 3. Electroporation of DNA into Cultured Cell Lines	55	(14)
Leah M. Cataldo
Zengyu Wang
Katya Ravid
Chapter 4. DNA Transfer to Blastula-Derived Cultures from ZebraFish	69	(24)
Paul Collodi
Chapter 5. Transfection of Rat Adipose Cells by Electroporation	93	(18)
Michael J. Quon
Chapter 6. Calcium Phosphate Transfection	111	(14)
K. J. Conn
A. Degterev
M. R. Fontanilla
C. B. Rich
J. A. Foster
Chapter 7. Optimization of Calcium Phosphate Transfection for Reporter Gene Analysis	125	(22)
John V. O'Mahoney
Timothy E. Adams
Chapter 8. Strontium Phosphate Transfection	147	(10)
Roger R. Reddel
Curtis C. Harris
Douglas E. Brash
Chapter 9. In Vitro Tissue Transfection by Calcium Phosphate	157	(22)
M. F. James
C. B. Rich
V. Trinkaus-Randall
J. A. Foster
Chapter 10. DEAE-Dextran Transfection	179	(14)
John J. Schwartz
Robert Rosenberg
Chapter 11. Cationic Liposomes	193	(20)
Vadim V. Bichko
Chapter 12. Transfection of Mammalian Cells with Yeast Artificial Chromosomes	213	(24)
William M. Strauss
Chapter 13. Mapping Human Senescence Genes Using Microcell-Mediated Chromosome Transfer	237	(28)
Robert F. Newbold
Andrew P. Cuthbert
Chapter 14. Methotrexate and DHFR Amplification	265	(18)
Michael H. Ricketts
List of Suppliers	283	(4)
Index	287