DNA Virus Replication

by Cann, Alan J.

• ISBN13: 9780199637126
• ISBN10: 0199637121
• Edition: 1st
• Format: Paperback
• Copyright: 2000-03-16
• Publisher: Oxford University Press

Summary

This book provides an overview of current research into DNA viruses. It is essential for all laboratories and students involved in DNA virus research.

Table of Contents

List of contributors	xiii
Abbreviations	xv
Macromolecular interactions in hepatitis B virus replication and particle assembly	1	(40)
Michael Nassal
Introduction	1	(2)
The HBV infectious cycle	3	(3)
Experimental systems to study HBV replication	6	(16)
Animal models	6	(4)
Transfection of cloned hepadnavirus DNA	10	(5)
Reconstituting hepadnavirus reverse transcription in vitro	15	(7)
Assembly of virus particles	22	(6)
Envelope containing particles	22	(1)
The nucleocapsid	23	(5)
The enigma of HBx	28	(2)
Conclusions and perspectives	30	(11)
Acknowledgements	31	(1)
References	31	(10)
Protein--protein interactions in papillomavirus replication	41	(25)
Dennis J. Mccance
Introduction	41	(1)
Early HPV proteins	42	(12)
HPV-16 E6	42	(3)
HPV-16 E7	45	(5)
HPV-16 E5	50	(2)
Summary of the roles of E6, E7, and E5 in keratinocyte differentiation and cell cycle control	52	(2)
Viral proteins involved in viral DNA replication	54	(3)
E1 and E2 proteins	55	(2)
Maintenance and amplification of HPV genomes	57	(1)
Summary	57	(9)
Acknowledgements	58	(1)
References	58	(8)
Molecular interactions in herpes simplex virus DNA replication	66	(39)
Nigel D. Stow
Introduction	66	(1)
Identification of the cis-acting signals and trans-acting proteins involved in HSV-1 DNA synthesis	67	(4)
Models for HSV-1 DNA replication	71	(3)
Molecular interactions involved in HSV-1 DNA synthesis	74	(1)
Interactions involved in unwinding the replication origins	74	(9)
ICP8	75	(1)
HSV-1 DNA replication origins	76	(2)
Interaction of UL9 with the viral origins	78	(2)
DNA helicase activity of UL9 and interaction with ICP8	80	(1)
Unwinding of the replication origins	81	(1)
Regulation of origin activity	82	(1)
Interactions involved in the initiation of DNA synthesis	83	(5)
The helicase--primase complex	83	(1)
Effect of ICP8 on the helicase--primase complex	84	(1)
The HSV-1 DNA polymerase holoenzyme	84	(3)
Interaction between UL8 and UL9	87	(1)
Interactions of the HSV-1 DNA polymerase with UL8 and UL9	87	(1)
Interaction of host DNA polymerase α with UL9	88	(1)
A model for the establishment of the HSV-1 replication fork	88	(3)
Other interactions involving HSV-1 ICP8	91	(1)
Intranuclear localization of HSV-1 DNA synthesis	92	(2)
Concluding remarks	94	(11)
References	94	(11)
Epstein--Barr virus proteins involved in cell immortalization	105	(28)
Martin Rowe
J. Eike Floettmann
Introuction	105	(1)
The nuclear proteins expressed in EBV-transformed B cells	106	(8)
The EBV nuclear antigen-1, EBNA1	106	(2)
The EBV nuclear antigen-2, EBNA2	108	(2)
The EBV leader protein, EBNA-LP	110	(1)
The EBV nuclear antigen-3 family, EBNA3B, EBNA3B, and EBNA3C	111	(3)
Latent membrane proteins expressed in EBV-transformed B cells	114	(5)
The latent membrane protein-1, LMP1	114	(4)
The latent membrane protein-2, LMP2	118	(1)
Conclusions	119	(14)
References	120	(13)
Molecular piracy by KSHV: a strategy of live and let live	133	(23)
Sukhanya Jayachandra
Yuan Chang
Patrick S. Moore
Introduction	133	(1)
KSHV as a model for molecular piracy	133	(2)
Immunomodulatory proteins encoded by KSHV	135	(8)
Viral interleukin-6 (vIL-6)	135	(2)
Viral macrophage inflammatory proteins (vMIPs)	137	(2)
Viral G protein-coupled receptor (vGCR)	139	(2)
Viral interferon regulatory factor (vIRF)	141	(2)
KSHV proteins affecting the cell cycle and apoptotsis	143	(3)
Viral cyclin (v-Cyc)	143	(1)
Viral Bcl-2 (vBcl-2)	144	(1)
Viral FLIP (vFLIP)	145	(1)
Novel ORFs encoded by KSHV	146	(1)
ORF K1	146	(1)
Kaposin	147	(1)
Conclusions	147	(9)
Acknowledgements	148	(1)
References	148	(8)
Cellular sites and mechanisms of human cytomegalovirus latency	156	(17)
John Sinclair
Introduction	156	(1)
Cells of the myeloid lineage are an important site of carriage of HCMV	157	(1)
Viral gene expression associated with latency	158	(1)
Permissiveness of peripheral blood cells for HCMV infection requires differentiation	159	(1)
Bone marrow---a reservoir of virus in the healthy carrier	160	(1)
Conditionally permissive cell lines as models for factors controlling latency and reactivation	161	(4)
Many cell types are infected during viraemia---does this reflect other sites of latency?	165	(1)
Conclusions	166	(7)
Acknowledgements	167	(1)
References	168	(5)
Control of transcription by adenovirus-E1A proteins	173	(27)
Alt Zantema
Alex J. Vander EB
Introduction	173	(1)
The adenovirus E1A proteins	173	(1)
Transcriptional regulation	174	(3)
Regulation of transcription initiation	175	(2)
Binding of E1A to the retinoblastoma and related proteins	177	(7)
Effect of pocket proteins on E2F	179	(2)
Binding of pRb to other cellular proteins	181	(3)
Binding of E1A to p300 and related proteins	184	(6)
Role of p300/CBP in transcription regulation	186	(1)
The intrinsic and associated acetyltransferase activity of p300/CBP	187	(1)
Role of p300/CBP in cell growth and differentiation	188	(1)
Role of the individual p300/CBP members	189	(1)
Other proteins associating with E1A	190	(10)
Acknowledgements	190	(1)
References	191	(9)
Adenovirus proteins that regulate apoptosis	200	(33)
William S. M. Wold
G. Chinnadurai
Introduction	200	(1)
Course of adenovirus infection	200	(4)
E3-coded proteins that inhibit immune-mediated apoptosis	204	(4)
Induction of apoptosis by E1A	208	(4)
Inhibition of apoptosis by the E1B-19K protein	212	(4)
Inhibition of apoptosis by the E1B-55K protein	216	(2)
Apoptosis regulation by E4 proteins	218	(1)
Inhibition of the antiviral effects of interferons	219	(1)
Induction of cell lysis and virus release by the adenovirus death protein (ADP)	220	(1)
Concluding remarks	220	(13)
Acknowledgements	222	(1)
References	222	(11)
Index	233