Drug Development

by Gaginella, Timothy S.; Guglietta, Antonio

• ISBN13: 9780896035898
• ISBN10: 0896035891
• Format: Hardcover
• Copyright: 1999-09-01
• Publisher: Humana Pr Inc

Summary

Timothy Gaginella and Antonio Guglietta bring together hands-on experts to critically review the many promising new areas of gastrointestinal pharmacology at the molecular level. Key chapters discuss such major themes as cyclooxygenase-2 inhibitors, the complexities of nitric oxide pathways in the gut, and the influence of cytokines on inflammatory bowel disease, as well as new opportunities for pharmacotherapy, including peptide growth factors, achykinins-particularly substance P and neurokinins-cholecystokinin receptors, and serotonin. Also examined as possible sites for treating motility disorders and visceral pain are the opioid receptors residing in the gut, and the H3-receptor agonists for treating gut inflammation and pain. Drug Development: Molecular Targets for Gastrointestinal Disease provides a comprehensive review of today's molecular pharmacological approaches to the discovery and development of novel drugs for gastrointestinal and liver diseases.

Author Biography

Giulio Bertaccini: Institute of Pharmacology, School of Medicine, University of Parma, Italy (deceased) Michel R. Briejer: Pharmacology Section, Yamanouchi Europe, The Netherlands Gabriella Coruzzi: Institute of Pharmacology, School of Medicine, University of Parma, Italy Massimo D'Amato: Department of Clinical Pharmacology, Rotta Research Laboratorium SpA, Monza (MI), Italy Claudio Fiocchi: Division of Gastroenterology, University Hospitals of Cleveland, Case Western Reserve University School of Medicine, Cleveland, OH Antonio Guglietta: Parke-Davis Pharmaceutical Research Division, Warner-Lambert Company, Ann Arbor, MI Peter Holzer: Department of Experimental and Clinical Pharmacology, University of Graz, Austria Jean-Louis Junien: Ferring SA, Paris, France Alan D. Levine: Division of Gastroenterology, University Hospitals of Cleveland, Case Western Reserve University School of Medicine, Cleveland, OH Francesco Makovec: Department of Preclinical Pharmacology, Rotta Research Laboratorium SpA, Monza (MI), Italy Giuseppina Morini: Institute of Pharmacology, School of Medicine, University of Parma, Italy Enzo Poli: Institute of Pharmacology, School of Medicine, University of Parma, Italy Pierre J. M. Riviere: Ferring Research Institute Inc., San Diego, CA Lucio C. Rovati, Department of Clinical Pharmacology, Rotta Research Laboratorium SpA, Monza (MI), Italy Sushil K. Sarna: Departments of Surgery and Physiology, Medical College of Wisconsin and Zablocki VA Medical Center, Milwaukee, WI Jan A. J. Schuurkes: Department of Gastrointestinal Pharmacology, Janssen Research Foundation, Beerse, Belgium B. L. Tepperman: Department of Physiology, University of Western Ontario, London, Canada Marija Veljaca: PLIVA d.d. Research Institute, Zagreb, Croatia. John L. Wallace: Department of Pharmacology and Therapeutics, University of Calgary, Alberta, Canada B. J. R. Whittle: The William Harvey Research Institute, St. Bartholomew's and the Royal London School of Medicine and Dentistry, London, UK

Table of Contents

Preface	v
Dedication	vii
Contributors	xi
Plant Cells and Tissues: Structure---Function Relationships	1	(26)
William V. Dashek
Methods for the Cytochemical/Histochemical Localization of Plant Cell/Tissue Chemicals	27	(10)
William V. Dashek
Methods in Light Microscope Radioautography	37	(22)
Alyce Linthurst
William V. Dashek
Some Fluorescence Microscopical Methods for Use with Algal, Fungal, and Plant Cells	59	(22)
Virginia A. Shepherd
Fluorescence Microscopy of Aniline Blue Stained Pistils	81	(6)
Renata Sniezko
A Short Introduction to Immunocytochemistry and a Protocol for Immunovisualization of Proteins with Alkaline Phosphatase	87	(14)
John E. Nielsen
The Fixation of Chemical Forms on Nitrocellulose Membranes	101	(12)
Rosannah Taylor
Dark-Field Microscopy and Its Application to Pollen Tube Culture	113	(8)
Tetsuya Higashiyama
Computer-Assisted Microphotometry	121	(26)
Howard J. Swatland
Isolation and Characterization of Endoplasmic Reticulum from Mulberry Cortical Parenchyma Cells	147	(14)
Norifumi Ukaji
Seizo Fujikawa
Shizuo Yoshida
Methods for the Identification of Isolated Plant Cell Organelles	161	(8)
William V. Dashek
Methods for the Detergent Release of Particle-Bound Plant Proteins	169	(16)
William V. Dashek
Scanning Electron Microscopy: Preparations for Diatoms	185	(10)
David W. Seaborn
Jennifer L. Wolny
Methods for the Ultrastructural Analysis of Plant Cells and Tissues	195	(20)
William V. Dashek
John E. Mayfield
Methods for Atomic Force and Scanning Tunneling Microscopies	215	(8)
William V. Dashek
Visualization of Golgi Apparatus by Zinc Iodide---Osmium Tetroxide (ZIO) Staining	223	(14)
Takako S. Kaneko
Mamiko Sato
Masako Osumi
Methods in Electron Microscope Autoradiography	237	(10)
William V. Dashek
Electron Microscopic Immunogold Localization	247	(16)
Eliot M. Herman
Microanalysis	263	(16)
Martin J. Hodson
The Use of Electron Microscopy in Molecular Biology	279	(14)
Roslyn A. March-Amegadzie
Summation	293
William V. Dashek
Index	295