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This book discusses the ion fluxes and electron transfer processes in biological membranes and artificial bio-films. It provides an in-depth description of the processes at the interface between the membrane/film and substrate electrode. Chapters include: ion transfer and ion channels in biological membranes in health and pathology; artificial membranes and films in electrochemical modelling of ionic fluxes in membrane potential formation; trasmembrane ion fluxes for lowering detection limits of ion-selective electrodes; voltage detection in ion channels and genetically-coded sensors; gating kinetics of voltage-dependent ion channels; connecting up to the surface: exploiting molecular wires for biosensing; electron transfer in and between redox proteins/enzymes, protein voltammetry--direct electron transfer between redox enzymes and electrodes; biological charge transfer reactions in mitochondria and illnesses related to mutations in the mitochondrion; electron transfer pathways between living bacterial cells and electrodes; biocomputing systems based on electrically wired enzymes; electron transfer pathways in photosystems, and reaction mechanism of multicopper blue oxidases related to their use in biodevices such as biofuel cells.