Emerging Tools for Single-Cell Analysis Advances in Optical Measurement Technologies

by Durack, Gary; Robinson, J. Paul

• ISBN13: 9780471315759
• ISBN10: 0471315753
• Edition: 1st
• Format: Hardcover
• Copyright: 2000-05-22
• Publisher: Wiley-Liss

Summary

The resurgence of interest in high-resolution evaluation of single-cell properties has led to examining where current technology stands at the beginning of a new millennium. Engineers and scientists have produced significant advances in cytometric technologies in just the past few years. Emerging Tools for Single-Cell Analysis: Advances in Optical Measurement Technologies stresses the applications and theories behind some of these advances in cell measurement and cell- sorting technologies.Rapid assessment of the proper function of cells and molecular processes within cells is essential. To that end, new and varying technologies present important diagnostic and prognostic tools relevant to a variety of diseases. Future developments in miniaturization of electronics, micro- and nanomachines, and biomedical engineering are certain to impact cell biology. New analytical technologies are revolutionizing our ability to functionally characterize, isolate, and manipulate single cells. This timely book offers researchers and design engineers much-needed information as they further develop technologies for cell analysis.By comparing and contrasting various approaches, the authors explain how those technologies converge toward similar goals: evaluating the properties of cells and sorting cells on those properties using optically-based measurement systems. Emerging Tools for Single-Cell Analysis offers scientists and engineers a vision of the exciting possibilities that exist as new technologies are applied to single-cell analysis,

Author Biography

Gary Durack is a biomedical engineer, inventor, and successful technology entrepreneur. Gary began his career at the Coulter Corporation, a world leader in IVD instrumentation.

J. Paul Robinson is the editor of Emerging Tools for Single-Cell Analysis: Advances in Optical Measurement Technologies, published by Wiley.

Table of Contents

Preface	vii
Contributors	ix
Cell-Sorting Technology	1	(20)
Gary Durack
High-Speed Cell Sorting	21	(28)
Ger van den Engh
Rare-Event Detection and Sorting of Rare Cells	49	(24)
James F. Leary
Applications of High-Speed Sorting for CD34+ Hematopoietic Stem Cells	73	(22)
Thomas Leemhuis
David Adams
Microfabricated Fluidic Devices for Single-Cell Handling and Analysis	95	(20)
David J. Beebe
Single DNA Fragment Detection by Flow Cytometry	115	(24)
Robert C. Habbersett
James H. Jett
Richard A. Keller
Fluorescence Lifetime Imaging: New Microscopy Technologies	139	(36)
Weiming Yu
William W. Mantulin
Enrico Gratton
Fluorescence Lifetime Flow Cytometry	175	(22)
John A. Steinkamp
Application of Fluorescence Lifetime and Two-Photon Fluorescence Cytometry	197	(24)
Donald J. Weaver Jr.
Gary Durack
Edward W. Voss Jr.
Anu Cherukuri
Probing Deep-Tissue Structures by Two-Photon Fluorescence Microscopy	221	(18)
Chen-Yuan Dong
Ki Hean Kim
Christof Buehler
Lily Hsu
Hyun Kim
Peter T. C. So
Barry R. Masters
Enrico Gratton
Irene E. Kochevar
Limits of Confocal Imaging	239	(32)
James B. Pawley
Scanning Near-Field Optical Imaging and Spectroscopy in Cell Biology	271	(20)
Vinod Subramaniam
Achim K. Kirsch
Attila Jenei
Thomas M. Jovin
White-Light Scanning Digital Microscopy	291	(16)
J. Paul Robinson
Ben Gravely
Illumination Sources	307	(16)
Howard M. Shapiro
Camera Technologies for Cytometry Applications	323	(16)
Kenneth Castleman
Index	339