Extracellular Matrix Protocols

by Streuli, Charles H.; Grant, Michael E.

• ISBN13: 9780896036246
• ISBN10: 0896036243
• Format: Hardcover
• Copyright: 2000-04-01
• Publisher: Humana Pr Inc

Summary

Leading bench-top experimentalists from around the world present, in a proven format, their state-of-the-art methods for probing the extracellular matrix (ECM), its mechanisms of assembly and how it influences cell behavior. These carefully detailed biochemical, biophysical, genetic, molecular biological, and cell biological techniques have been optimized for the study of ECM structure and assembly, the mechanisms of cell-ECM interactions, and the molecular genetics of ECM proteins and defects in human disease, and bring to a wider audience a range of highly sophisticated methods that have hitherto been difficult even for a few specialized laboratories. Cutting-edge and highly practical, Extracellular Matrix Protocols offers today's ECM researchers a gold-standard collection of powerful, readily reproducible methods to investigate the extracellular matrix and the altered cell-ECM interactions that play so important a role in human health and disease.

Author Biography

Vitali Alexeev: Department of Dermatology and Cutaneous Biology, Jefferson Medical College, Philadelphia, PA Fiorella Altruda: Universita di Torino, Italy Nicholas C. Avery: Division of Molecular and Cellular Biology, University of Bristol, Langford, Bristol, UK Allen J. Bailey: Division of Molecular and Cellular Biology, University of Bristol, Langford, Bristol, UK Mara Brancaccio: Universita di Torino, Italy Michael D. Briggs: School of Biological Sciences, University of Manchester, UK Jeremy R. Bright: Department of Biochemistry, University of Oxford, UK Neil J. Bulleid: School of Biological Sciences, University of Manchester, UK Iain D. Campbell: Department of Biochemistry, University of Oxford, UK Christopher S. Chen: Childrens Hospital, Harvard Medical School, Boston, MA David R. Clemmons: Department of Medicine, University of North Carolina, Chapel Hill Sarah L. Dallas: Department of Medicine, University of Texas Health Science Center at San Antonio Daniel E. Emerling: Department of Molecular and Cell Biology, University of California, Berkeley Jurgen Engel: Biocenter of the University of Basel, Switzerland Eva-Maria Erb: Biocenter of the University of Basel, Switzerland Jose Maria Frade: Department of Neurobiochemistry, Max Planck Institute of Neurobiology, Martinsried, Germany Charles ffrench-Constant: Wellcome Trust Cancer Research Campaign Institute, University of Cambridge, UK Emma E. Frost: Wellcome Trust Cancer Research Campaign Institute, University of Cambridge, UK Emiliana Giacomello: Division for Experimental Oncology, The Reference Center for Oncology, Aviano, Italy Chris J. Gilpin: School of Biological Sciences, University of Manchester, UK Michael E. Grant: School of Biological Sciences, University of Manchester, UK Helen K. Graham: School of Biological Sciences, University of Manchester, UK Philip Gribbon: School of Biological Sciences, University of Manchester, UK Timothy Hardingham: School of Biological Sciences, University of Manchester, UK Emilio Hirsch: Universita di Torino, Italy David F. Holmes: School of Biological Sciences, University of Manchester, UK Martin J. Humphries: School of Biological Sciences, University of Manchester, UK Donald E. Inger: Childrens Hospital, Harvard Medical School, Boston, MA Karl E. Kadler: School of Biological Sciences, University of Manchester, UK Michele Kedinger: INSERM, Strasbourg, France Paul D. Kemp: Stockport, UK Cay M. Kielty: School of Biological Sciences, University of Manchester, UK Teresa C. M. Klinowska: School of Biological Sciences, University of Manchester, UK Paul Krebsbach: National Institute of Health, Bethesda, MD Arthur D. Lander: Department of Molecular and Cell Biology, University of California, Berkley Friedrich Laub: Brookdale Center for Developmental and Molecular Biology, Mt. Sinai Hospital, New York Ying Liu: National Institute of Health, Bethesda, MD Todd L. Mathus: Robert Wood Johnson Medical School, Piscataway, NJ Roger S. Meadows: School of Biological Sciences, University of Manchester, UK Barbara Merkl: Institute for Biochemistry, University of Cologne, Germany Richard Milner: Wellcome Trust Cancer Research Campaign Institute, University of Cambridge, UK Gradimir N. Misevic: Universite des Sciences et Technologies de Lille, Villeneuve D'Ascq, France Paul A. Mould: School of Biological Sciences, University of Manchester, UK Johanna Myllyharju: Collagen Research Unit, University of Oulu, Finland Uwe Odenthal: Institute for Biochemistry, University of Oxford, UK Emanuele Ostuni: Childrens Hospital, Harvard Medical School, Boston, MA Mats Paulsson: Institute for Biochemistry, University of Cologne, Germany Roberto Perris: Division for Experimental Oncology, The Reference Center for Oncology, Aviano, Italy Andrew R. Pickford: Department of Biochemistry, University of Oxford, UK Jennifer R. Potts: Department of Biochemistry, University of Oxford, UK Francesco Ramirez: Brookdale Center for Developmental and Molecular Biology, Mount Sinai Hospital, New York Anthony Ratcliffe: Advanced Tissue Sciences, La Jolla, CA Ronda E. Schreiber: Advanced Tissue Sciences, La Jolla, CA Michael J. Sherratt: School of Biological Sciences, University of Manchester, UK Patricia Simon-Assmann: INSERM, Strasbourg, France Trevor J. Sims: Division of Molecular and Cellular Biology, University of Bristol, UK Neil Smyth: Institute for Biochemistry, University of Cologne, Germany Paola Spessotto: Division for Experimental Oncology, The Reference Center for Oncology, Aviano, Italy Charles H. Streuli: School of Biological Sciences, University of Manchester, UK Hideaki Sumiyoshi: Brookdale Center for Developmental and Molecular Biology, Mount Sinai Hospital, New York Alfredo Rodriguez Tebar: Department of Neurobiochemistry, Max Planck Institute of Neurobiology, Martinsried, Germany Noriyuki Tsumaki: National Institute of Health, Bethesda, MD George M. Whitesides: Childrens Hospital, Harvard Medical School, Boston, MA Richard R. Wilson: School of Biological Sciences, University of Manchester, UK Yoshihiko Yamada: National Institute of Health, Bethesda, MD Kyonggeun Yoon: Department of Dermatology and Cutaneous Biology, Jefferson Medical College, Philadelphia, PA Peter D. Yurchenco: Robert Wood Johnson Medical School, Piscataway, NJ Bo Zheng: Department of Medicine, University of California, Berkeley

Table of Contents

Preface	v
Contributors	xi
Part I. Biochemistry of Extracellular Matrix
Semipermeabilized Cells to Study Procollagen Assembly	1	(10)
Richard R. Wilson
Neil J. Bulleid
Quantitative Determination of Collagen Crosslinks	11	(16)
Trevor J. Sims
Nicholas C. Avery
Allen J. Bailey
Analysis of Laminin Structure and Function with Recombinant Glycoprotein Expressed in Insect Cells	27	(12)
Todd L. Mathus
Peter D. Yurchenco
Recombinant Collagen Trimers from Insect Cells and Yeast	39	(10)
Johanna Myllyharju
Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Strep II Tag	49	(10)
Neil Smyth
Uwe Odenthal
Barbara Merkl
Mats Paulsson
Part II. Biophysics of Extracellular Matrix Proteins
Preparation of Isotopically Labeled Recombinant Fragments of Fibronectin for Functional and Structural Study by Heteronuclear Nuclear Magnetic Resonance Spectroscopy	59	(12)
Jeremy R. Bright
Andrew R. Pickford
Jennifer R. Potts
Iain D. Campbell
Reconstitution of Functional Integrin into Phopsholipid Vesicles and Planar Lipid Bilayers	71	(12)
Eva-Maria Erb
Jurgen Engel
Confocal-FRAP Analysis of ECM Molecular Interactions	83	(12)
Timothy Hardingham
Philip Gribbon
Electron Cryomicroscopy of Fibrillar Collagens	95	(16)
Roger S. Meadows
David F. Holmes
Chris J. Gilpin
Karl E. Kadler
Atomic Force Microscopy Measurements of Intermolecular Binding Strength	111	(8)
Gradimir N. Misevic
ECM Macromolecules: Rotary Shadowing and Scanning Transmission Electron Microscopy	119	(14)
Michael J. Sherratt
Helen K. Graham
Cay M. Kielty
David F. Holmes
Part III. Molecular Biology of Extracellular Matrix Genes
Screening for Mutations in Cartilage ECM Genes	133	(14)
Michael D. Briggs
Tissue-Specific KO of ECM Proteins	147	(32)
Emilio Hirsch
Mara Brancaccio
Fiorella Altruda
Homologous Gene Targeting to Study ECM Assembly	179	(8)
Francesco Ramirez
Friedrich Laub
Hideaki Sumiyoshi
Enhancer Analysis of the α1(II) and α2(XI) Collagen Genes in Transfected Chondrocytes and Transgenic Mice	187	(10)
Noriyuki Tsumaki
Ying Liu
Yoshihiko Yamada
Paul Krebsbach
Retroviral Delivery of ECM Genes to Cells	197	(12)
Kyonggeun Yoon
Vitali Alexeev
Part IV. Cell Biology of the Extracellular Matrix
Using Self-Assembled Monolayers to Pattern ECM Proteins and Cells on Subtrates	209	(12)
Christopher S. Chen
Emanuele Ostuni
George M. Whitesides
Donald E. Ingber
Methods for Preparing Extracellular Matrix and Quantifying Insulin-like Growth Factor-Binding Protein Binding to the ECM	221	(10)
Bo Zheng
David R. Clemmons
Measuring Interactions Between ECM and TGFβ-Like Proteins	231	(14)
Sarah L. Dallas
Using Organotypic Tissue Slices as Substrata for the Culture of Dissociated Cells	245	(12)
Daniel E. Emerling
Arthur D. Lander
Neuroepithelial Differentiation Induced by ECM Molecules	257	(8)
Jose Maria Frade
Alfred Rodriguez Tebar
Migration Assays for Oligodendrocyte Precursor Cells	265	(14)
Emma E. Frost
Richard Milner
Charles ffrench-Constant
Cell Adhesion Assays	279	(8)
Martin J. Humphries
Tissue Engineering and Cell-Populated Collagen Matrices	287	(8)
Paul D. Kemp
Solid Phase Assays for Studying ECM Protein-Protein Interactions	295	(6)
A. Paul Mould
Tissue Engineering of Cartilage	301	(10)
Ronda E. Schreiber
Anthony Ratcliffe
Tissue Recombinants to Study Extracellular Matrix Targeting to Basement Membranes	311	(10)
Patricia Simon-Assmann
Michele Kedinger
Fluorescence Assays to Study Cell Adhesion and Migration In Vitro	321	(24)
Paola Spessotto
Emiliana Giacomello
Roberto Perris
Analyzing Cell-ECM Interactions in Adult Mammary Gland by Transplantation of Embryonic Mammary Tissue from Knockout Mice	345	(14)
Teresa C. M. Klinowska
Charles H. Streuli
Index	359