Flow Cytometry with Plant Cells Analysis of Genes, Chromosomes and Genomes

by Dolezel, Jaroslav; Greilhuber, Johann; Suda, Jan

• ISBN13: 9783527314874
• ISBN10: 3527314873
• Edition: 1st
• Format: Hardcover
• Copyright: 2007-04-09
• Publisher: Wiley-VCH

Summary

Targeted at beginners as well as experienced users, this handy reference explains the benefits and uses of flow cytometery in the study of plants and their genomes. Following a brief introduction that highlights general considerations when analyzing plant cells by flow cytometric methods, the book goes on to discuss examples of application in plant genetics, genomic analysis, cell cycle analysis, marine organism analysis and breeding studies. With its list of general reading and a glossary of terms, this first reference on FCM in plants fills a real gap by providing first-hand practical hints for the growing community of plant geneticists.

Author Biography

Jaroslav Dolezel is the Head of the Czech Institute of Experimental Botany in Olomouc, and Associate Professor at the Palacky University in Olomouc. He has pioneered the use of flow cytometry in plant science for the analysis of nuclear genome size and regularly holds international training courses on the subject.

Johann Greilhuber is Associate Professor at the Institute of Botany at the University of Vienna. He has studied plant genomes for almost 30 years and is an expert on intraspecific variation of genome size. He has constantly been developing novel techniques to study plant genomes, among them many methods based on flow cytometry.

Jan Suda heads the laboratory of flow and image cytometry at the Czech Academy of Sciences' Institute of Botany. He is a taxonomist specializing in plant biosystematics, including genome size determination and has co-authored a book on taxonomy analysis.

Table of Contents

Preface

List of Contributors

Cytometry and Cytometers: Development and Growth

Overview

Origins

From Absorption to Fluorescence, from Imaging to Flow

The Growth of Multiparameter Flow Cytometry

Bench-tops and Behemoths: Convergent Evolution

Image Cytometry: New Beginnings?

References

Principles of Flow Cytometry

Overview

Introduction

A Brief History of Flow Cytometry

Components of a Flow Cytometer

Flow Cytometric Informatics

Spectral Compensation

Cell Sorting

Calibration Issues

Conclusions

References

Flow Cytometry with Plants: an Overview

Overview

Introduction

Fluorescence is a Fundamental Parameter

Pushing Plants through the Flow Cytometer

Application of Flow Cytometry in Plants

A Flow Cytometer in Every Laboratory?

Conclusions and Future Trends

References

Nuclear DNA Content Measurement

Overview

Introduction

Nuclear DNA Content: Words, Concepts and Symbols

Units for Presenting DNA Amounts and their Conversion Factors

Sample Preparation for Flow Cytometric DNA Measurement

Standardization

Fluorescence Inhibitors and Coatings of Debris

Quality Control and Data Presentation

Future Directions

References

Flow Cytometry and Ploidy: Applications in Plant Systematics, Ecology and Evolutionary Biology

Overview

Introduction

Practical Considerations

Applications in Plant Systematics

Applications in Plant Ecology and Evolutionary Biology

Future Directions

References

Reproduction Mode Screening

Overview

Introduction

Analyses of the Mode of Reproduction

A Recent Innovative Method: the Flow Cytometric Seed Screen

Flow Cytometry with Mature Seeds for other Purposes

Conclusions

References

Genome Size and its Uses: the Impact of Flow Cytometry

Overview

Introduction

Why is Genome Size Important?

What is Known about Genome Size in Plants?

The Extent of Genome Size Variation across Plant Taxa

Understanding the Consequences of Genome Size Variation: Ecological and Evolutionary Implications

Methods of Estimating Genome Size in Plants and the Impact of Flow Cytometry

Recent Developments and the Future of Flow Cytometry in Genome Size Research

References

DNA Base Composition of Plant Genomes

Overview

Introduction

Analysis of Base Composition by Flow Cytometry

Conclusions

References

Detection and Viability Assessment of Plant Pathogenic Microorganisms using Flow Cytometry

Overview

Introduction

Viability Assessment

Immunodetection

Conclusions and Future Prospects

References

Protoplast Analysis using Flow Cytometry and Sorting

Overview

Introduction

Results of Protoplast Analyses using Flow Cytometry and Sorting

Walled P

Table of Contents provided by Publisher. All Rights Reserved.