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This references discusses major advances in the development of novel imaging techniques that exploit new probes. It describes the photophysical properties of new fluorescent probes, particularly the important red fluorescent probes from corals. The book gives a detailed account of state-of-the-art imaging methods that exploit the characteristics of new probes. It also illustrates the applications of fluorescent probes in a variety of different optical techniques, including superresolution microscopy. Many figures throughout the text further clarify concepts.