Gene Therapy Protocols

by Morgan, Jeffrey R.

• ISBN13: 9780896038691
• ISBN10: 0896038696
• Edition: 2nd
• Format: Spiral Bound
• Copyright: 2002-01-01
• Publisher: HUMANA PRESS

Summary

In this fully updated and revised 2nd edition of Gene Therapy Protocols, leading experts from academic and industrial laboratories around the world detail their most effective viral and nonviral methods of gene transfer, as well as discuss their applications in different organ systems. These methods range from those in which new molecular conjugates show great promise for targeting targeting gene transfer and regulating transgene expression, to those used in such exciting applications as the delivery of therapeutic proteins, vaccination, and tissue engineering. Up-to-date and highly practical, Gene Therapy Protocols, 2nd Edition, offers a rich compilation of the revolutionary advances that have recently occurred in gene transfer technology, with each article providing proven the step-by-step laboratory procedures that enable its successful therapeutic application.

Table of Contents

Preface	v
Contributors	xi
Poly-L-Lysine-Based Gene Delivery Systems: Synthesis, Purification, and Application	1	(14)
Charles P. Lollo
Mariusz G. Banaszcyk
Patricia M. Mullen
Christopher C. Coffin
Dongpei Wu
Alison T. Carlo
Donna L. Bassett
Erin K. Gouveia
Dennis J. Carlo
Targeted Gene Transfer to Liver Using Protein-DNA Complexes	15	(10)
Catherine H. Wu
Cherie M. Walton
George Y. Wu
Receptor-Directed Molecular Conjugates for Gene Transfer	25	(24)
Assem G. Ziady
Pamela B. Davis
Gene Transfer into Muscle by Electroporation in Vivo	49	(14)
Jun-ichi Miyazaki
Hiroyuki Aihara
Viral Liposomes: Preparation and Use	63	(10)
Yasufumi Kaneda
LPD Nanoparticles-Novel Nonviral Vector for Efficient Gene Delivery	73	(10)
Yadi Tan
Mark Whitmore
Song Li
Peter Frederik
Leaf Huang
Solvoplex Synthetic Vector for Intrapulmonary Gene Delivery: Preparation and Use	83	(12)
Klaus Schughart
Ulla B. Rasmussen
Gene Correction Frequency by Chimeric RNA-DNA Oligonucleotide Using Nuclear Extracts	95	(14)
Olga A. Igoucheva
Kyonggeun Yoon
Regulated Expression of Plasmid-Based Gene Therapies	109	(14)
Ronald V. Abruzzese
Fiona C. MacLaughlin
Louis C. Smith
Jeffrey L. Nordstrom
Photochemical Transfection: Light-Induced, Site-Directed Gene Delivery	123	(14)
Lina Prasmickaite
Anders Hogset
Kristian Berg
Direct Gene Transfer and Vaccination Via Skin Transefection Using a Gene Gun	137	(12)
Chia-Feng Kuo
Jeng Hwan Wang
Ning-Sun Yang
Preparation of Pseudotyped Retroviral Vector	149	(12)
Hong Yu
Quantitative Measurement of the Concentration of Active Recombinant Retrovirus	161	(12)
Stelios T. Andreadis
Jeffrey R. Morgan
Minigene-Containing Retroviral Vectors Using an Alphavirus/Retrovirus Hybrid Vector System: Production and Use	173	(14)
Jarmo Wahlfors
Richard A. Morgan
Retrovirus-Mediated Gene Transfer to Human Hematopoietic Stem Cells	187	(16)
William P. Swaney
Enrico M. Novelli
Alfred B. Bahnson
John A. Barranger
Genetically Modified Skin Substitutes: Preparation and Use	203	(16)
Karen E. Hamoen
Gulsun Erdag
Jennifer L. Cusick
Hinne A. Rakhorst
Jeffrey R. Morgan
Bioartificial Muscles in Gene Therapy	219	(14)
Courtney Powell
Janet Shansky
Michael Del Tatto
Herman H. Vandenburgh
Cytokine Gene-Modified Cell-Based Cancer Vaccines	233	(26)
R. Todd Reilly
Jean-Pascal H. Machiels
Leisha A. Emens
Elizabeth M. Jaffee
HIV-Based Vectors: Preparation and Use	259	(16)
Antonia Follenzi
Luigi Naldini
Packaging Cell System for Lentivirus Vectors: Preparation and Use	275	(28)
Narasimhachar Srinivasakumar
Lentiviral Vectors: Preparation and Use	303	(16)
Lung-Ji Chang
Anne-Kathrin Zaiss
Simian Foamy Virus Vectors: Preparation and Use	319	(16)
Jeonghae Park
Ayalew Mergia
Recombinant Feline Immunodeficiency Virus Vectors: Preparation and Use	335	(16)
Michael A. Curran
Garry P. Nolan
Lentivirus Vector Based on Simian Immunodeficiency Virus: Development and Use	351	(10)
Klaus Uberla
Cytoplasmic RNA Vector Derived from Nontransmissible Sendai Virus: Producation and Use	361	(10)
Akihiro Lida
Mamoru Hasegawa
Preparation of Helper-Dependent Adenoviral Vectors	371	(18)
Philip Ng
Robin J. Parks
Frank L. Graham
Construction of First-Generation Adenoviral Vectors	389	(26)
Philip Ng
Frank L. Graham
Preparation of Ovine Adenovirus Vectors	415	(12)
Peter Loser
Daniel Kumin
Moritz Hillgenberg
Gerald W. Both
Christian Hofmann
Highly Purified Recombinant Adeno-Associated Virus Vectors: Preparation and Quantitation	427	(18)
Bruce Schnepp
K. Reed Clark
High-Titer Stocks of Adeno-Associated Virus from Replicating Amplicons and Herpes Vectors	445	(16)
Ajay R. Mistry
Mahesh De Alwis
Elisabeth Feudner
Robin R. Ali
Adrian J. Thrasher
Herpes Simplex Virus/Adeno-Associated Virus Hybrid Vectors for Gene Transfer to Neurons: Preparation and Use	461	(20)
Lauren C. Costantini
Cornel Fraefer
Xandra O. Breakefield
Ole Isacson
Development of Replication-Defective Herpes Simplex Virus Vectors	481	(28)
William F. Goins
David M. Krisky
Darren P. Wolfe
David J. Fink
Joseph C. Glorioso
Index	509