Genetics Laboratory Investigations

by Mertens, Thomas L; Hammersmith, Robert L.

• ISBN13: 9780131742529
• ISBN10: 0131742523
• Edition: 13th
• Format: Paperback
• Copyright: 2006-06-13
• Publisher: Benjamin Cummings

Summary

THEdefinitive genetics lab manual for over 50 years, this user-friendly volume stresses classical genetics, but includes some of the recent advances related to molecular and human genetics as well.Drosophilaand Maize Experiments in Genetics: Monohybrid Crosses; Dihybrid Crosses. Cell Reproduction: Mitosis. Meiosis in Animals: Oogenesis and Spermatogenesis. Meiosis in Angiosperms: Microsporogenesis and Megasporogenesis. Polytene Chromosomes fromDrosophilaSalivary Glands. Sex Chromosomes and Gene Transmission. The Sex Check: A Study of Sex Chromatin in Human Cells. Human Chromosomes. Linkage and Crossing Over. Genetics of Ascospore Color inSordaria: An Investigation of Linkage and Crossing Over Using Tetrad Analysis. Open-Ended Experiments UsingDrosophila: Locating a Mutant Gene in Its Chromosome. Isolation of DNA. Restriction Endonuclease Digestion and Gel Electrophoresis of DNA. Amplification of DNA Polymorphisms by Polymerase Chain Reaction (PCR) and DNA Fingerprinting. Transformation ofEscherichia coli.Gene Action: Synthesis of ... b-Galactosidase inEscherichia coli.Chromatographic Characterization ofDrosophilamelanogaster Mutants. Bacterial Mutagenesis. Gene Recombination in Phage. Polygenic Inheritance: Fingerprint Ridge Count. Population Genetics: The Hardy-Weinberg Principle; The Effects of Selection and Genetic Drift. Applied Human Genetics.For anyone interested in hands-on genetics work.

Table of Contents

Preface	v
Drosophila and Maize Experiments in Genetics: Monohybrid and Dihybrid Crosses	1	(18)
Principles of Probability	19	(8)
The Chi-Square Test	27	(14)
Cell Reproduction: Mitosis	41	(8)
Meiosis in Animals: Oogenesis and Spermatogenesis	49	(8)
Meiosis in Angiosperms: Microsporogenesis	57	(14)
Polytene Chromosomes from Drosophila Salivary Glands	71	(4)
Sex Chromosomes and Gene Transmission	75	(10)
The Sex Check: A Study of Sex Chromatin in Human Cells	85	(6)
Human Chromosomes	91	(32)
Linkage and Crossing Over	123	(20)
Genetics of Ascospore Color in Sordaria: An Investigation of Linkage and Crossing Over Using Tetrad Analysis	143	(8)
Open-Ended Experiments Using Drosophila: Locating a Mutant Gene in Its Chromosome	151	(6)
The Genetic Material: Isolation of DNA	157	(6)
Restriction Endonuclease Digestion and Gel Electrophoresis of DNA	163	(16)
The Amplification of DNA Polymorphisms by Polymerase Chain Reaction (PCR) and DNA Fingerprinting	179	(16)
Transformation of Escherichia coli	195	(10)
Gene Action: Synthesis of β-Galactosidase in Escherichia coli	205	(6)
Chromatographic Characterization of Drosophila melanogaster Mutants	211	(8)
Bacterial Mutagenesis	219	(6)
Gene Recombination in Phage	225	(6)
Polygenic Inheritance: Fingerprint Ridge Count	231	(10)
Population Genetics: The Hardy-Weinberg Principle	241	(12)
Population Genetics: The Effects of Selection and Genetic Drift	253	(8)
Applied Human Genetics	261	(20)
NCBI and Genomic Data Mining	281	(18)
Supplemental Laboratory Topics	299	(1)
Supplement 1 Plant Speciation by Interspecific Hybridization and Allotetraploidy	299	(2)
Supplement 2 Chromosome Translocations in Rhoeo spathacea	301	(1)
Supplement 3 Human Pedigree Analysis	302