Guide to Techniques in Glycobiology

by Abelson; Simon; Lennarz; Hart

• ISBN13: 9780121821319
• ISBN10: 0121821315
• Format: Hardcover
• Copyright: 1994-01-03
• Publisher: Elsevier Science

Summary

The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. Key Features * Detailed procedures newly written for this volume * Extensive practical information * Rationale and strategies for carbohydrate chain analysis Among the topics covered: * Release of Oligosaccharides from Glycoproteins by Hydrazinolysis * Mass Spectrometry of Carbohydrate-Containing Biopolymers * High-resolution Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Reducing Saccharides * Capillary Electrophoresis of Oligosaccharides * Synthesis and Uses of Azido-Substituted Nucleoside Diphosphate Sugar Photoaffinity Analogs * Structural Analysis of Glycosylphosphatidylinositol Anchors * Identification of Polysialic Acids in Glycoconjugates

Table of Contents

Carbohydrate Composition Analysis of Glycoconjugates by Gas-Liquid Chromatography/Mass Spectrometry

Metabolic Radiolabeling of Glycoconjugates

Nonmetabolic Radiolabeling and Tagging of Glyconconjugates

Enzymatic Deglycosylation of Asparagine-Linked Glycans: Purification, Properties, and Specificity of Oligosaccharide-Cleaving Enzymes from Flavobacterium meningosepticum

Release of Oligosaccharides from Glycoproteins by Hydrazinolysis

Use of Lectins in Analysis of Glycoconjugates

Saccharide Linkage Analysis Using Methylation and Other Techniques

Mass Spectrometry of Carbohydrates-Containing Bipolymers

1H Nuclear Magnetic Resonance Spectroscopy of Carbohydrate Chains of Glycoproteins

Determination of Sialic Acids

Size Fractionation of Oligosaccharides

High-pH Anion-Exchange Chromatography of Glycoprotein-Derived Carbohydrates

High-Performance Liquid Chromatography of Pyridylaminated Saccharides

High-Performance Liquid Chromatography of Oligosaccharides

High-Resolution Polyacrylamide Gel Electrophoresis of Fluorophore-Labeled Reducing Saccharides

Capillary Electrophoresis of Oligosaccharides

Glycosidases in Structural Analysis

Glycosyltransferases in Glycobiology

Glycosidase Inhibitors in Study of Glycoconjugates

Synthesis and Use of Azido-Substituted Nucleoside Diphosphate Sugar Photoaffinity Analogs

Glycoform Analysis of Glycoproteins

Isolation of Glycosphingolipids

Thin-Layer Chromatography of Glycosphingolipids

Isolation and Characterization of Proteoglycans

Structural Analysis of Glycosylphosphatidylinositol Anchors

Detection of O-Linked N-Acetylglucosamine (O-GlcNAc) on Cytoplasmic and Nuclear Proteins

Identification of Polysialic Acids in Glycoconjugates

Neoglycolipds: Probes of Oligosaccharide Structure, Antigenicity, and Function

Author Index

Subject Index

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