Handbook of Cell-Penetrating Peptides, Second Edition

by Langel; Ulo

• ISBN13: 9780849350900
• ISBN10: 0849350905
• Edition: 2nd
• Format: Hardcover
• Copyright: 2006-08-15
• Publisher: CRC Press

Summary

Since the first Handbook of Cell-Penetrating Peptides was prepared in 2001, the wealth of new information on the use of these peptides as transport systems has in fact served to confound the field. The constant internal change in the field of cell-penetrating peptides (CPPs) is due to recent research uncovering apparent ambiguities in cellular uptake. There is still neither a common terminology nor a uniform explanation for the penetrative mechanism of cell-penetrating peptides.In this second edition of the Handbook of Cell-Penetrating Peptides, the authors summarize the current state of the field including recent reevaluations of earlier studies of CPP mechanisms. Beginning with an overview of the classes of peptides and their individual uptake mechanisms, from the earlier lipid models to the more recent endocytotic pathways, the book demonstrates the diversity and the opportunity for these biologically active proteins to serve as future drug leads. The text then covers the use of CPPs in gene modulation, addressing the application of antisense and decoy oligonucleotides, as well as the new avenue of research targeting specific tumors and other tissues-questions that had barely been asked when the first edition was published.By summarizing the diffuse information regarding CPPs, including the ambiguities and variety of mechanisms, the Handbook of Cell-Penetrating Peptides provides the most solid foundation available from which to expand the potential of this rapidly growing field of medicine.

Table of Contents

Classes and prediction of cell-penetrating peptides	p. 1
Penetratins	p. 5
Tat-derived cell-penetrating peptides : discovery, mechanism of cell uptake, and applications to the delivery of oligonucleotides	p. 29
Model amphipathic peptides	p. 43
Cell-penetrating cis-[gamma]-amino-L-proline-derived peptides	p. 61
Prediction of cell-penetrating peptides	p. 77
Mechanisms of cell-penetrating peptides	p. 87
Inverted lipid models : a pathway for peptide internalization?	p. 89
Membrane interactions of cell-penetrating peptides	p. 109
Interactions of cell-penetrating peptides with model membranes	p. 139
The import mechanism of cationic cell-penetrating peptides and its implications for the delivery of peptide inhibitors of signal transduction	p. 161
Mechanisms of cargo delivery by transportans	p. 183
Tat-mediated peptide/protein transduction in vivo	p. 201
Proteoglycans as endocytosis receptors for CPPs	p. 219
Functionality of shuttling proteins and protein-derived peptides	p. 235
Cell permeable peptides and messenger proteins, from a serendipitous observation to a new signaling mechanism	p. 239
Pharmacology and in vivo efficacy of pepducins in hemostasis and arterial thrombosis	p. 245
HOXB4 homeoprotein transfer promotes the expansion of hematopoietic stem cells	p. 259
Applications of cell-penetrating peptides as signal transduction modulators	p. 273
SynB vectors as drug carriers	p. 293
Applications of cell-penetrating peptides in gene modulation and protein transport	p. 309
Peptide conjugates of oligonucleotide analogs and siRNA for gene expression modulation	p. 313
Lego-like strategy for assembling multivalent peptide-based vehicles able to deliver molecular cargoes into cells	p. 329
Mechanistic insights into guanidinium-rich transporters entering cells	p. 353
Cell-penetrating short interfering RNAs and decoy oligonucleotides	p. 375
A noncovalent peptide-based strategy for peptide and short interfering RNA delivery	p. 387
Selective targeting to tumors and other tissues with cell-penetrating peptides : in vivo applications	p. 409
Selective delivery to vascular addresses : in vivo applications of cell-type-specific cell-penetrating peptides	p. 413
In vivo applications of cell-penetrating peptides	p. 423
Intracellular delivery of nanoparticles with CPPs	p. 439
Strategies to incorporate CPPs into synthetic viruses for tumor-targeted gene therapy	p. 455
Cell-permeable penta-peptides derived from bax-inhibiting peptide	p. 469
Cellular uptake of liposomal and micellar carriers mediated by ApoE-derived peptides	p. 483
Imaging the influx of cell-penetrating peptides into the cytosol of individual live cells	p. 505
Microbial membrane-penetrating peptides and their applications	p. 515
CPP methods	p. 535
Design and synthesis of cell-penetrating peptides	p. 537
Toxicity methods for cell-penetrating peptides	p. 553
Methods to study the translocation of cell-penetrating peptides	p. 567
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