Handbook of Nucleic Acid Purification

by Liu; Dongyou

• ISBN13: 9781420070965
• ISBN10: 1420070967
• Format: Hardcover
• Copyright: 2009-01-14
• Publisher: CRC Press

Summary

As a result of complex cellular structures and functions, purification of nucleic acids often represents a vital first step in the study of molecular biology of living organisms as well as in the evolutionary/phylogenetic analysis of ancient specimens. To this end, many innovative nucleic acids isolation methods have been designed, which are scattered over a variety of professional books and journals.This project will be an all encompassing volume on nucleic acid purification strategies for bacteria, viruses, fungi, parasites, mammals and plants, with an additional emphasis on sample preparation methods for direct molecular applications. Each chapter will comprise a concise review of historical background and purification principles, a detailed description of current techniques (including several state-or-art protocols for large and small scale preparations) and a discussion for future development trends.

Table of Contents

Preface	p. ix
Editor	p. xi
Contributors	p. xiii
Nucleic Acids: Structures, Functions, and Applications	p. 1
Purification of Nucleic Acids from Viruses
Isolation of Viral DNA from Cultures	p. 23
Isolation of Viral RNA from Cultures	p. 41
Preparation of Viral Samples for Direct Molecular Applications	p. 61
Purification of Nucleic Acids from Bacteria
Isolation of Bacterial DNA from Cultures	p. 85
Isolation of Bacterial RNA from Cultures	p. 107
Isolation of Plasmids	p. 127
Preparation of Bacterial Samples for Direct Molecular Applications	p. 147
Purification of Nucleic Acids from Fungi
Isolation of Nucleic Acids from Yeasts	p. 171
Isolation of Nucleic Acids from Filamentous Fungi	p. 191
Preparation of Fungal Specimens for Direct Molecular Applications	p. 211
Purification of Nucleic Acids from Parasites and Insects
Isolation of Nucleic Acids from Protozoa	p. 253
Isolation of Nucleic Acids from Helminthes	p. 275
Isolation of Nucleic Acids from Insects	p. 297
Preparation of Parasitic Specimens for Direct Molecular Applications	p. 317
Purification of Nucleic Acids from Mammals
Isolation of Nucleic Acids from Body Fluids	p. 381
Isolation of Nucleic Acids from Soft Tissues	p. 405
Isolation of Nucleic Acids from Hard Tissues	p. 427
Isolation of DNA from Ancient Samples	p. 449
Preparation of Forensic Samples for Direct Molecular Applications	p. 471
Purification of Nucleic Acids from Plants
Isolation of Nucleic Acids from Plants	p. 503
Isolation of Megabase-Sized DNA Fragments from Plants	p. 521
Purification of Nucleic Acids from Miscellaneous Sources
Purification of Nucleic Acids from Gels	p. 537
Index	p. 555
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