Hepatocytes

by Maurel, Patrick; Brechot, Christian

• ISBN13: 9781607616870
• ISBN10: 1607616874
• Edition: 1st
• Format: Hardcover
• Copyright: 2010-04-20
• Publisher: Humana Pr Inc

Summary

Hepatocytes account for approximately 80% of the liver mass and play a significant role in various aspects of liver physiopathology, exhibiting unrivaled complexity and diversity of functions. In Hepatocytes: Methods and Protocols, expert researchers provide the reader with methods, technical protocols, and review chapters focusing on selected areas of hepatocyte biology including isolation, culture, differentiation and stem cells, and hepatocyte use in clinical, basic, and applied research. With a specific emphasis on human hepatocytes, the volume presents chapters covering subjects including hepatocyte culture models, cryopreservation methods, differentiation assessment, liver ontogenesis, production of hepatocytes from stem cells, drug/xenobiotic metabolism, toxicity and transport, bile acid and blood coagulation factor production, infection by HBV and HCV, humanized animals, biortificial liver devices, hepatocyte transplantation. As a volume in the highly successful Methods in Molecular Biology'„¢ series, protocol chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Hepatocytes: Methods and Protocols will be useful to all those who are currently using or planning to use human, or animal, hepatocytes to investigate any aspect of liver physiopathology or who are interested in liver development or liver stem cells and liver biotherapy.

Table of Contents

Foreword	p. v
Preface	p. vii
Contributors	p. xv
General Review on In Vitro Hepatocyte Models and Their Applications	p. 1
Human Foetal Hepatocytes: Isolation, Characterization, and Transplantation	p. 41
Isolation and Culture of Primary Hepatocytes from Resected Human Liver Tissue	p. 57
Optimisation of the Cryopreservation of Primary Hepatocytes	p. 83
Cryopreservation of Human Hepatocytes for Clinical Use	p. 107
Hepatocyte Differentiation	p. 115
Reversible Manipulation of Apoptosis Sensitivity in Cultured Hepatocytes by Matrix-Mediated Manipulation of Signaling Activities	p. 139
Markers and Signaling Factors for Stem Cell Differentiation to Hepatocytes: Lessons from Developmental Studies	p. 157
Hepatic Stem Cells	p. 167
Hepatic Stem Cells and Liver Development	p. 181
Generation of Hepatocytes from Human Embryonic Stem Cells	p. 237
Isolation and Culture of Adult Human Liver Progenitor Cells: In Vitro Differentiation to Hepatocyte-Like Cells	p. 247
The HepaRG Cell Line: Biological Properties and Relevance as a Tool for Cell Biology, Drug Metabolism, and Virology Studies	p. 261
Transdifferentiation of Pancreatic Cells to Hepatocytes	p. 273
Evaluation of Drug Metabolism, Drug-Drug Interactions, and In Vitro Hepatotoxicity with Cryopreserved Human Hepatocytes	p. 281
The Use of Human Hepatocytes to Investigate Drug Metabolism and CYP Enzyme Induction	p. 295
The Use of Hepatocytes to Investigate UDP-Glucuronosyltransferases and Sulfotransferases	p. 309
The Use of Hepatocytes to Investigate Drug Uptake Transporters	p. 327
Metabonomic Studies on Human Hepatocyte in Primary Culture	p. 355
The Application of HepRG Cells in Evaluation of Cytochrome P450 Induction Properties of Drug Compounds	p. 375
The Use of Hepatocytes to Investigate Drug Toxicity	p. 389
The Use of Human Hepatocytes to Investigate Bile Acid Synthesis	p. 417
Use of Human Hepatocytes to Investigate Blood Coagulation Factor	p. 431
Use of Human Hepatocytes to Investigate HCV Infection	p. 447
The Use of Hepatocytes to Investigate HDV Infection: The HDV/HepaRG Model	p. 463
Rodent Models of Liver Repopulation	p. 475
Chimeric Mice with Humanized Liver: Tools for the Study of Drug Metabolism, Excretion, and Toxicity	p. 491
Bioartificial Liver Support Systems	p. 511
Human Hepatocyte Transplantation	p. 525
Subject Index	p. 535
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