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9781588290052

Hormone Assays in Biological Fluids

by ;
  • ISBN13:

    9781588290052

  • ISBN10:

    1588290050

  • Format: Hardcover
  • Copyright: 2006-01-16
  • Publisher: Humana Pr Inc

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Summary

Expert researchers who have developed and applied significant new assays describe in step-by-step detail a variety of methods for measuring a broad variety of hormones, related peptides, and synthetic steroids in various biological fluids. The hormones measured range from glucocorticoids in biological fluids, urinary steroids, aldosterone in blood, and plasma renin activity, to gut hormones in plasma, melatonin, prolactin, 6-sulfatoxymelatonin, and androgens in blood, saliva, and hair. The emphasis is on noncommercial assays so that investigators can set up novel methods suited to their special needs. Commercial assays are also described for comparative purposes. Tutorials on radioimmunoassay, gas chromatography-mass spectrometry, high-performance liquid chromatography, and PCR techniques help the reader to choose the best method for his or her purpose.

Table of Contents

Preface v
Contributors ix
Immunoassay Techniques
1(24)
Michael J. Wheeler
High-Performance Liquid Chromatography for Hormone Assay
25(28)
John W. Honour
Gas Chromatography-Mass Spectrometry
53(22)
John W. Honour
An Introduction to PCR Techniques
75(16)
Gill Rumsby
Methods for the Investigation of Thyroid Function
91(18)
Christine R. Squire
Assays for LH, FSH, and Prolactin
109(16)
Michael J. Wheeler
Adrenocorticotropic Hormone and Related Peptides
125(16)
Les A. Perry
Measurement of Glucocorticoids in Biological Fluids
141(18)
Geoff Holder
Urinary Steroid Profiling
159(18)
Norman F. Taylor
Measurement of Aldosterone in Blood
177(10)
Marion L. Cawood
Measurement of Plasma Renin Activity
187(10)
Marion L. Cawood
Measurement of Androgens
197(16)
Michael J. Wheeler
Measurement of Gut Hormones in Plasma
213(22)
Shahrad Taheri
Mohammad Ghatei
Steve Bloom
Measurement of Melatonin and 6-Sulphatoxymelatonin
235(20)
Benita Middleton
Index 255

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