Laboratory Applications in Microbiology: A Case Study Approach

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  • Edition: 3rd
  • Format: Spiral Bound
  • Copyright: 2/21/2014
  • Publisher: McGraw-Hill Education

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Laboratory Applications in Microbiology: A Case Study Approach includes a photo atlas with more than 250 full-color images! This lab uses real-life case studies as the basis for exercises in the laboratory. This is the only microbiology lab manual focusing on this means of instruction, an approach particularly applicable to the microbiology laboratory. The author has carefully organized the exercises so that students develop a solid intellectual base beginning with a particular technique, moving through the case study, and finally applying new knowledge to unique situations beyond the case study.

Table of Contents

Case Study Exercise 1 Safety Considerations in theMicrobiology Laboratory

Case Study Exercise 2 Microscopy and Measurement of Microscopic Specimens

Case Study Exercise 3 Identification and Classification of Algae

Case Study Exercise 4 Survey of Medically ImportantProtozoa

Case Study Exercise 5 Identification andClassification of Fungi

Case Study Exercise 6 Ubiquity ofMicroorganisms

Case Study Exercise 7 Aseptic and Pure Culture Techniques

Case Study Exercise 8 Simple Staining, Negative Staining, and Gram Staining

Case Study Exercise 9 Endospore Staining

Case Study Exercise 10 Acid-Fast and Endospore Staining

Case Study Exercise 11 Acid-Fast Staining

Case Study Exercise 12 Viable Plate Count

Case Study Exercise 13 Cultivation of Anaerobes

Case Study Exercise 14 Temperature Effects onBacterial Growth and Survival

Case Study Exercise 15 pH and Microbial Growth

Case Study Exercise 16 Effects of Osmotic Pressureon Bacterial Growth

Case Study Exercise 17 Lethal Effects of UltravioletLight

Case Study Exercise 18 Evaluation of Disinfectants

Case Study Exercise 19 Effectiveness of HandScrubbing

Case Study Exercise 20 Antimicrobic SensitivityTesting: Kirby-Bauer, Tube Dilution, and E-TestMethods

Case Study Exercise 21 Phage Typing of Bacteria

Case Study Exercise 22 Simulated Epidemic

Case Study Exercise 23 Morbidity and MortalityWeekly Report

Case Study Exercise 24 Bacterial Transformation

Case Study Exercise 25 The Ames Test

Case Study Exercise 26 DNA Extraction fromBacterial Cells

Case Study Exercise 27 DNA Profiling

Case Study Exercise 28 Measures of Water Quality:Most Probable Number Procedure

Case Study Exercise 29 Measures of Water Quality:Membrane Filtration Method

Case Study Exercise 30 Measures of Milk Quality:Methylene Blue Reductase Test

Case Study Exercise 31 Bacterial Counts of Food

Case Study Exercise 32 Isolation and Identification of Staphylococci

Case Study Exercise 33 Isolation and Identification of Streptococci

Case Study Exercise 34 Epidemiology of Gastrointestinal Illness: Differentiation of Enterobacteriaceae

Case Study Exercise 35 Blood Typing

Case Study Exercise 36 Differential White Blood

Case Study Exercise 37 Slide Agglutination

Case Study Exercise 38 Enzyme-LinkedImmunosorbent Assay (ELISA)

Case Study Exercise 39 Identification of BacterialUnknowns

Exercise 40 Colony Morphology

Exercise 41 Growth in Solid and Liquid Media

Exercise 42 Motilty Methods: Wet Mount andHanging Drop

Exercise 43 Flagella Stain

Exercise 44 Streak-Plate Isolation

Exercise 45 Loop Dilution

Exercise 46 Spread-Plate

Exercise 47 Fluid Thioglycollate Medium

Exercise 48 Mannitol Salt Agar

Exercise 49 MacConkey Agar

Exercise 50 Desoxycholate Agar

Exercise 51 Endo Agar

Exercise 52 Eosin Methylene Blue Agar

Exercise 53 Hektoen Enteric Agar

Exercise 54 Xylose Lysine Desoxycholate Agar

Exercise 55 Blood Agar

Exercise 56 Motility Medium

Exercise 57 SIM Medium

Exercise 58 Kligler's Iron Agar

Exercise 59 Triple Sugar Iron Agar

Exercise 60 Lysine Iron Agar

Exercise 61 Litmus Milk

Exercise 62 Oxidation-Fermentation Test

Exercise 63 Phenol Red Broth

Exercise 64 Purple Broth

Exercise 65 Methyl Red and Voges-ProskauerTests

Exercise 66 Catalase Test

Exercise 67 Oxidase Test

Exercise 68 Nitrate Reduction Test

Exercise 69 Coagulase Test

Exercise 70 Citrate Test

Exercise 71 Malonate Test

Exercise 72 Decarboxylation Test

Exercise 73 Phenylalanine Deaminase Test

Exercise 74 Bile Esculin Test

Exercise 75 Starch Hydrolysis

Exercise 76 ONPG Test

Exercise 77 Urease Test

Exercise 78 Casease Test

Exercise 79 Gelatinase Test

Exercise 80 DNase Test

Exercise 81 Lipase Test

Exercise 82 CAMP Test

Exercise 83 PYR Test

Exercise 84 API 20E System

Exercise 85 Enterotube II System

Exercise 86 Antibiotic Disk Sensitivity Tests

Exercise 87 B-Lactamase Test

Exercise 88 Viable Plate Count

Exercise 89 Direct Cell Count

Appendix A: Spectrophotometric Determination of BacterialGrowth: Use of the Spectrophotometer

Appendix B: Use of Pipettes in the Laboratory

Appendix C: Preparation of Culture Media

Appendix D: Media, Reagents, and Stain Formulas

Appendix E: Data Sheet for Unknown Identification

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