Manual of Diagnostic Antibodies for Immunohistology

by Anthony S-Y. Leong , Kumarasen Cooper , F. Joel W-M. Leong

• ISBN13: 9781841101002
• ISBN10: 1841101001
• Edition: 2nd
• Format: Hardcover
• Copyright: 2002-12-01
• Publisher: Greenwich Medical Media

Summary

The rapid acceptance of immunohistochemistry as an important and even indispensable adjunct to morphological examination and diagnosis requires the modern anatomical pathology laboratory to be conversant with, and proficient in, immunostaining procedures, as well as methods of tissue processing and antigen retrieval and the underlying characteristics of the increasing number of antibodies and antisera available. This fully revised, expanded and updated edition provides a comprehensive list of antisera and monoclonal antibodies that have useful diagnostic applications in tissue sections and cell preparations. Various clones, which are commercially available to detect the same antigen, are listed and the sensitivities and specificities of the antibodies are discussed. Importantly, the authors, all pathologists with a wealth of experience in immunostaining procedures, provide fully referenced details and expert advice on each reagent, which the reader will find invaluable. Finally, the appendices provide easily accessible and clear summaries of selected antibody panels for specific diagnostic situations, details of heat-induced antigen/epitope retrieval (including the use of microwaves) and a useful reference to the websites of the main antibody suppliers.

Author Biography

F. Joel W.-M. Leong is Clinical Lecturer in Pathology, Nuffield Department of Clinical Laboratory Sciences, University of Oxford, UK.

Table of Contents

Preface to the First Edition	p. v
Preface to the Second Edition	p. vii
Introduction	p. xiii
Antibodies
[alpha]-Smooth Muscle Actin ([alpha]-SMA)	p. 3
[alpha]-1-antichymotrypsin	p. 5
[alpha]-1-antitrypsin	p. 7
[alpha]-Fetoprotein (AFP)	p. 11
Amyloid	p. 13
Androgen receptor	p. 17
Anti-apoptosis	p. 19
Anti-p80 (ALK-NMP fusion protein)	p. 23
Bcl-2	p. 25
Bcl-6	p. 29
Ber-EP4	p. 31
[beta]-hCG (Human Chorionic Gonadotropin)	p. 33
CA 125	p. 35
N/97-Cadherin/E-Cadherin	p. 37
Calcitonin	p. 41
Calponin	p. 43
Calretinin	p. 45
Candida Albicans	p. 49
Carcinoembryonic Antigen (CEA)	p. 51
Catenins, [alpha], [beta], [gamma]	p. 53
Cathepsin D	p. 55
CD 1	p. 59
CD 2	p. 61
CD 3	p. 63
CD 4	p. 65
CD 5	p. 67
CD 7	p. 71
CD 8	p. 73
CD 9	p. 75
CD 10 (CALLA)	p. 77
CD 103	p. 79
CD 11	p. 81
CD 15	p. 83
CD 19	p. 87
CD 20	p. 89
CD 21	p. 93
CD 23	p. 95
CD 24	p. 97
CD 30 (Ki-1)	p. 99
CD 31	p. 103
CD 34	p. 105
CD 35	p. 109
CD 38	p. 111
CD 40	p. 113
CD 43	p. 115
CD 44	p. 117
CD 45 (Leucocyte Common Antigen)	p. 121
CD 54 (ICAM-1)	p. 125
CD 56 (Neural Cell Adhesion Molecule)	p. 127
CD 57	p. 131
CD 68	p. 135
CD 74 (LN2)	p. 139
CD w75 (LN1)	p. 141
CD 79a	p. 143
CD99 (p30/32[superscript MIC2])	p. 145
CD117 (KIT)	p. 149
c-erbB-2 (Her-2, neu)	p. 153
Chlamydia	p. 157
Chromogranin	p. 159
c-Myc	p. 161
Collagen Type IV	p. 163
Cyclin D1 (bcl-1)	p. 165
Cytokeratins	p. 167
Cytokeratin 20 (CK 20)	p. 171
Cytokeratin 7 (CK 7)	p. 173
Cytokeratins-MNF 116	p. 175
Cytokeratins-CAM 5.2	p. 177
Cytokeratins-AE1/AE3	p. 179
Cytokeratins-MAK-6	p. 181
Cytokeratins-34[beta]E12	p. 183
Cytokeratin 5/6 (CK 5/6)	p. 185
Other Pan-cytokeratin Cocktails	p. 187
Cytomegalovirus (CMV)	p. 189
Cytotoxic Molecules (TIA-1, Granzyme B, Perforin)	p. 191
DBA.44 (Hairy Cell Leukemia)	p. 195
Desmin	p. 197
Desmoplakins	p. 201
Epidermal Growth Factors: TGF-[alpha] AND EGFR	p. 203
Epithelial Membrane Antigen (EMA)	p. 205
Epstein-Barr Virus, LMP	p. 209
Erythropoietin	p. 213
Estrogen Receptor (ER)	p. 215
Factor VIII RA (von Willebrand factor)	p. 219
Factor XIIIa	p. 221
Fas (CD95) and Fas-ligand (CD95L)	p. 223
Fascin	p. 225
Ferritin	p. 227
Fibrin	p. 229
Fibrinogen	p. 231
Fibronectin	p. 233
FLI-1 protein	p. 235
FMC-7	p. 237
Glial Fibrillary Acidic Protein (GFAP)	p. 239
Gross Cystic Disease Fluid Protein-15 (GCDFP-15, BRST-2)	p. 241
HAM56 (Macrophage marker)	p. 245
HBME-1 (Mesothelial Cell)	p. 247
h-Caldesmon	p. 249
Heat shock proteins (Hsps)	p. 251
Helicobacter pylori	p. 253
Hep Par 1 (Hepatocyte marker)	p. 255
Hepatitis B Core Antigen (HBcAG)	p. 257
Hepatitis B Surface Antigen (HBsAG)	p. 259
Herpes Simplex Virus I & II (HSV I & II)	p. 261
HLA-DR	p. 263
HMB-45 (Melanoma marker)	p. 265
hMLH1 and hMSH2 - Mismatch repair proteins	p. 269
Human Immunodeficiency Virus (HIV)	p. 273
Human Milk Fat Globule (HMFG)	p. 275
Human Papilloma Virus (HPV)	p. 277
Human Parvovirus B19	p. 279
Human Placental Lactogen (hPL)	p. 281
Immunoglobulins: Ig[kappa], Ig[lambda], IgA, IgD, IgE, IgG, IgM	p. 283
Inhibin	p. 287
Ki-67 (MIB 1, Ki-S5)	p. 289
Laminin	p. 293
Lysozyme (muramidase)	p. 297
MAC 387 (Macrophage marker)	p. 299
MART-1/Melan-A	p. 301
Maspin	p. 303
MDM-2 protein	p. 305
Measles	p. 307
Mel-CAM (CD146)	p. 309
Metallothioneins	p. 311
Micropthalmia transcription factor (MiTF)	p. 313
Mitochondria	p. 315
MOC-31	p. 317
Muscle Specific Actin (MSA)	p. 319
Mycobacterial Antigen	p. 321
Myelin Basic Protein (MBP)	p. 323
Myeloperoxidase	p. 325
MyoD1	p. 327
Myogenin	p. 331
Myoglobin	p. 333
Neurofilaments	p. 335
Neuron-Specific Enolase (NSE)	p. 337
Neutrophil elastase	p. 339
nm23/NME1	p. 341
Osteopontin	p. 345
P27[superscript kip1]	p. 347
p53	p. 349
Pancreatic Hormones (insulin, somatostatin, vasoactive intestinal polypeptide, gastrin, glucagon, pancreatic polypeptide)	p. 351
Parathyroid Hormone-Related Protein (PTHrP)	p. 357
Parathyroid hormone	p. 361
P-glycoprotein (P-170), Multi-Drug Resistance (MDR)	p. 363
Pituitary Hormones (ACTH, FSH, HGH, LH, PRL, TSH)	p. 365
Placental Aklaline Phosphatase (PLAP)	p. 369
Pneumocystis carinii	p. 371
Pregnancy-specific [beta]-1-glycoprotein (SP1)	p. 373
Progesterone Receptor (PR)	p. 375
Proliferating Cell Nuclear Antigen (PCNA)	p. 377
Prostate-Specific Antigen (PSA)	p. 379
Prostatic Acid Phosphatase (PAP)	p. 381
Protein Gene Product 9.5 (PGP 9.5)	p. 383
pS2	p. 385
Rabies	p. 387
Retinoblastoma Gene Protein (P110[superscript RB], Rb protein)	p. 389
S100	p. 391
Serotonin	p. 395
Simian Virus 40 (SV40 T antigen)	p. 397
Smooth muscle myosin-heavy chain	p. 399
Spectrin/Fodrin	p. 401
Surfactant apoprotein-A	p. 403
Synaptophysin	p. 405
TAG 72 (B72.3)	p. 407
Tau	p. 409
Tenascin	p. 411
Terminal Deoxynucleotidyl Transferase (TdT)	p. 413
Thrombomodulin	p. 417
Thyroglobulin	p. 419
Thyroid Transcription Factor-1 (TTF-1)	p. 421
Topoisomerase Ilalpha	p. 425
Toxoplasma gondii	p. 427
Tyrosinase	p. 429
Ubiquitin	p. 431
Ulex Europaeus Agglutinin 1 Lectin (UEA-I)	p. 433
VEGF (Vascular Endothelial Growth Factor)	p. 435
Villin	p. 437
Vimentin	p. 439
Vinculin	p. 443
VS38	p. 445
WT1	p. 447
Appendices
Selected antibody panels for specific diagnostic situations	p. 451
Bone/Soft tissue - Chondroid-like Tumors	p. 453
Brain - Metastatic Carcinoma vs Glioblastoma vs Meningioma	p. 453
Childhood Round Cell Tumors	p. 453
Gastrointestinal and Aerodigestive Tract Mucosa - Basaloid Squamous vs Adenoid Cystic vs Neuroendocrine Carcinoma	p. 453
Gonads - Germ Cell Tumors versus Somatic Adenocarcinoma	p. 454
Granulocytic sarcoma versus lymphoma versus carcinoma versus plasmacytoma	p. 454
Intracranial Tumors	p. 454
Liver - Hepatocellular Carcinoma vs Metastatic Carcinoma vs Cholangiocarcinoma	p. 454
Lung - Clear Cell Tumors	p. 455
Lymph Node - Round Cell Tumors in Adults	p. 455
Undifferentiated Round Cell Tumors	p. 455
Mediastinal tumors	p. 455
Nasal Tumors	p. 456
Pelvis - Metastatic Colonic Adenocarcinoma vs Ovarian Endometrioid Carcinoma	p. 456
Perineum - Prostatic vs Bladder vs Rectal Carcinoma	p. 456
Peritoneum - Myxoid Tumors	p. 456
Pleura - Mesothelioma vs Carcinoma	p. 457
Retroperitoneum - Renal Cell Carcinoma vs Adrenocortical Carcinoma vs Pheochromocytoma	p. 457
Retroperitoneum - Vacuolated/Clear Cell Tumor	p. 457
Skin - Adnexal Tumors	p. 457
Skin - Basal Cell Carcinoma vs Squamous Carcinoma vs Adnexal Carcinoma	p. 458
Skin - Pagetoid Tumors	p. 458
Skin - Spindle Cell Tumors	p. 458
Soft Tissue - Epithelioid Tumors	p. 459
Soft Tissue - Spindle Cell, fasciculated	p. 459
Soft Tissue - Myxoid Tumors	p. 459
Soft Tissue - Pleomorphic Tumors	p. 460
Extraskeletal Myxoid/Chondroid Tumors	p. 460
Stomach - Undifferentiated Spindle Cell Tumors	p. 460
Thyroid carcinomas	p. 460
Urinary Tract - Spindle Cell Proliferations	p. 461
Uterine cervix - Endometrial versus Endocervical Carcinoma	p. 461
Uterus - Trophoblastic Cells	p. 461
Uterus - Immunophenotyping of Syncytiotrophoblasts in Trophoblastic Proliferations	p. 462
Brain Intraventricular Tumors	p. 462
CNS Small Cell Tumors	p. 462
Tissue-Associated Antigens in "Treatable Tumors"	p. 463
Epithelial Tumors Which May Coexpress Vimentin Intermediate Filaments*	p. 463
Mesenchymal Tumors which may Coexpress Cytokeratin	p. 464
Tumors which may co-express three or more intermediate filaments	p. 464
Abbreviations to antibodies and their sources	p. 465
Antibody Panels for Lymphoid Neoplasms	p. 467
Useful Markers in B-cell Neoplasms	p. 467
Useful Markers of T-cell Neoplasms	p. 467
Markers of Reed Sternberg cells*	p. 468
Useful markers of Monocytes/macrophages	p. 468
Markers of Myeloid Cells	p. 468
Useful Markers of Natural Killer- (NK) cells	p. 469
Markers of langerhans Cells and Interdigitating Reticulum Cells	p. 469
Markers of Follicular Dendric Cells	p. 469
Panel for Small Cell Lymphomas	p. 469
Classic Hodgkin's Disease vs Lymphocyte Predominant Hodgkin's Disease vs T-cell/histiocyte-rich B-cell Lymphoma	p. 470
Antibody Applications	p. 471
Suppliers
Suppliers	p. 479
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