Membrane Protein Crystallization

by DeLucas

• ISBN13: 9780123749871
• ISBN10: 0123749875
• Edition: 1st
• Format: Hardcover
• Copyright: 2009-06-22
• Publisher: Elsevier Science

Summary

This volume of Current Topics in Membranes focuses on Membrane Protein Crystallization, beginning with a review of past successes and general trends, then further discussing challenges of mebranes protein crystallization, cell free production of membrane proteins and novel lipids for membrane protein crystallization. This publication also includes tools to enchance membrane protein crystallization, technique advancements, and crystallization strategies used for photosystem I and its complexes, establishing Membrane Protein Crystallization as a needed, practical reference for researchers.

Table of Contents

Contributors	p. xi
Preface	p. xv
Previous Volumes in Series	p. xvii
Introduction	p. 1
Introduction to the Crystallization of Biological Macromolecules
Overview	p. 5
Introduction	p. 6
The Requirement for Supersaturation	p. 6
References	p. 22
In Vitro Synthesis of Posttranslationally Modified Membrane Proteins
Overview	p. 25
Introduction	p. 26
Materials and Methods	p. 29
Results	p. 30
Conclusions	p. 46
References	p. 47
Harnessing Photosynthetic Bacteria for Membrane Protein Production
Overview	p. 52
Introduction	p. 52
Foreign Gene Expression in Rhodobacter sphaeroides	p. 57
Membrane Protein Preparations for Structural and Functional Studies	p. 68
Practical Aspects of Heterologous Protein Expression in Rhodobacter	p. 73
Concluding Statements	p. 77
References	p. 78
Monoacylglycerols: The Workhorse Lipids for Crystallizing Membrane Proteins in Mesophases
Overview	p. 83
Introduction	p. 84
In Meso Crystallogenesis in Molecular Detail	p. 86
Mesophase Behavior: From Lamellar to Cubic and Sponge Phases	p. 87
A Central Role for Lipid	p. 91
Lipid Synthesis and Purification	p. 95
Conclusions	p. 105
References	p. 106
Practical Aspects of Membrane Proteins Crystallization in Bicelles
Overview	p. 109
Introduction	p. 110
Bicelles	p. 112
Phase Behavior of Bicelles	p. 113
Examples of Membrane Proteins Crystallized in Bicelles	p. 114
Crystal Packing	p. 118
Practical Information	p. 118
Conclusion	p. 122
References	p. 123
Membrane Protein Crystallization: Approaching the Problem and Understanding the Solutions
Overview	p. 128
Introduction	p. 128
The Basic Crystallization Process	p. 128
Vapor Diffusion	p. 131
Microdialysis	p. 133
Microfluidics	p. 134
Counter-Diffusion	p. 134
Novel Methods using Lipids in Crystallization	p. 135
How to obtain Crystals from a Membrane Protein of Choice	p. 137
Optimization of Initial "Hits"	p. 139
Seeding	p. 140
Is it Possible to Preselect Crystallization Conditions That are Inherently More Favorable for Crystallization?	p. 144
Conclusions	p. 145
References	p. 146
Tools to Enhance Membrane Protein Crystallization
Overview	p. 152
Introduction	p. 152
Protein-Protein Interactions and Crystallization	p. 153
Self-Interaction Chromatography	p. 156
Validation Studies: SIC Accurately Quantifies B	p. 158
References	p. 175
Advances in Microfluidic Membrane Protein Crystallization Techniques
Overview	p. 179
Membrane Protein Crystallization	p. 180
Microfluidics	p. 180
Crystallization by FID in Polydimethylsiloxane (PDMS) Devices	p. 182
Membrane Protein Crystallization in Gradients Established in Microchannels	p. 184
Plug-Based Membrane Protein Crystallization in Microcapillaries	p. 185
Conclusion	p. 187
References	p. 189
Crystallization of Photosynthetic Membrane Proteins
Overview	p. 192
Overview of Crystallization of Photosynthetic Membrane Proteins	p. 192
Biological Parameters that Influence Crystallization	p. 196
Physical-Chemical Parameters that Influence Crystallization	p. 202
Crystallization Techniques	p. 209
Determination of Phase Diagrams	p. 216
Seeding Techniques	p. 221
References	p. 224
A Practical Guide to X-Ray Crystallography of ß-Barrel Membrane Proteins: Expression, Purification, Detergent Selection, and Crystallization
Overview	p. 230
Introduction	p. 230
Expression and Purification	p. 232
Preliminary Crystallization and Diffraction-Based Optimization	p. 252
Materials and Methods	p. 262
References	p. 263
Bacterial Membrane Proteins: The New Soluble Proteins?
Overview	p. 269
Structures of Omps	p. 270
Recombinant Production of Omps	p. 284
Recombinant Bacterial Inner Membrane Proteins and Eukaryotic Membrane Proteins	p. 286
Crystallization of Membrane Proteins	p. 287
New Strategies for Eukaryotic Proteins from the Study of GPCR	p. 288
Summary	p. 291
References	p. 292
Index	p. 299
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