Methods for General and Molecular Microbiology

by Reddy, C. A.; Beveridge, T. J.; Breznak, J. A.; Marzluf, G. A.; Schmidt, T. M.

• ISBN13: 9781555812232
• ISBN10: 1555812236
• Edition: 3rd
• Format: Hardcover
• Copyright: 2007-07-01
• Publisher: Amer Society for Microbiology

Summary

Michigan State Univ., East Lansing, MI. Presents traditional methods of microbiology and commonly used modern molecular microbiological methods. Topics include an introduction to morphology and ultrastructure, light microscopy, electron microscopy, nutrition and media, and more. For microbiologists and students. Previous edition by Gerhardt, c1994. DNLM: Microbiological Techniques.

Table of Contents

Morphology and ultrastructure	p. 1
Introduction to morphology and infrastructure	p. 3
Light microscopy	p. 5
Sampling and staining for light microscopy	p. 19
Laser scanning microscopy	p. 34
Electron microscopy	p. 54
Computational image analysis and reconstruction from transmission electron micrographs	p. 82
Atomic force microscopy	p. 96
Cell fractionation	p. 108
Antigen-antibody reactions	p. 138
Growth	p. 169
Introduction	p. 171
Growth measurement	p. 172
Nutrition and media	p. 200
Enrichment and isolation	p. 215
Culture techniques	p. 270
Energetics, stoichiometry, and kinetics of microbial growth	p. 286
Physicochemical factors in growth	p. 309
Phenotypic characterization and the principles of comparative systematics	p. 330
General methods to investigate microbial symbioses	p. 394
Metabolism	p. 421
Introduction to metabolism	p. 423
Physical analysis and purification methods	p. 424
Chemical analysis	p. 462
Enzymatic activity	p. 504
Permeability and transport	p. 527
Bacterial respiration	p. 539
Carbohydrate fermentations	p. 558
Metabolism of aromatic compounds	p. 586
Cellulases, hemicellulases, and pectinases	p. 596
Lignin and lignin-modifying enzymes	p. 611
Molecular genetics	p. 621
Introduction to molecular genetics	p. 623
Similarity analysis of DNAs	p. 624
Nucleic acid analysis	p. 653
Measuring spontaneous mutation rates	p. 676
Transposon mutagenesis	p. 684
Plasmids	p. 709
Gene transfer in gram-negative bacteria	p. 735
Genetic exchange in gram-positive bacteria	p. 756
Genetics of archaea	p. 800
Genetic manipulations using phages	p. 825
Community and genomic analysis	p. 839
Introduction to community and genomic analysis	p. 841
Characterization of bacterial genome sequences by similarity searching	p. 842
Reconstructing and interpreting evolutionary relationships	p. 856
Microbial nucleotide fingerprints in nature	p. 869
Construction of BAC and fosmid libraries from naturally occurring microbial populations	p. 879
Single cell identification by fluorescence in situ hybridization	p. 886
Measurement of rRNA abundance by hybridization with oligodeoxynucleotide probes	p. 897
Analysis of microbial communities with denaturing gradient gel electrophoresis and terminal restriction fragment length polymorphism	p. 909
Mycology	p. 925
Introduction to mycology	p. 927
Methods for studying terrestrial fungal ecology and diversity	p. 929
Physiology, metabolism, and molecular aspects of filamentous fungi	p. 952
Microbiological and genetic methods for filamentous fungi	p. 965
Principles and practice of DNA microarray technology	p. 978
Laboratory safety	p. 997
Culture preservation	p. 1019
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