Methods in Neurosciences Vol. 8 : Neurotoxins

by Conn, P. Michael

• ISBN13: 9780121852665
• ISBN10: 0121852660
• Format: Hardcover
• Copyright: 1992-03-01
• Publisher: Academic Pr

Summary

The exquisite simplicity and potency of toxins have made them valuable probes of neural systems. This book presents a comprehensive compilation of techniques used for the preparation, handling, and, particularly, for the use of neurotoxins. Model systems are described in which these neurotoxins have been extremely valuable in developing an understanding of the cellular and molecular basis of secretion and electrophysiological events leading to altered cell function. Key Features * Convenient benchtop format * Methods presented for easy adaptation to new systems * A virtual "A-B-C" of commonly used and available toxins * Comprehensive protocols included for the use of alpha toxin, apamin, batrachotoxin; botulina toxin, bungarotoxin; channel ligands: agonists and antagonists; capsaicin; charybdotoxin, ciguatoxin; Clostridium botulinum neurotoxin; cholera toxin (choleragen); conotoxin; dendrotoxin; endothelin; fasciculin; geographutoxin; latrotoxin; natural toxins; neosurgatoxin; palytoxin; pertussis toxin; resiniferatoxin; sarafotoxin; scorpion toxin; snake venom toxins

Table of Contents

Comparison of Alpha Toxin of Staphylococcus and Aerolysin for Formation of Ion-Permeable Channels

Apamin: A Probe for Low Conductance, Calcium-Activated Potassium Channels

Batrachotoxinin A [3H]Benzoate Binding to Sodium Channels

Botulinal Neurotoxins: Mode of Action on Neurotransmitter Release

Botulinum Toxin as a Tool in Neurobiology

*ga-Bungarotoxin Receptor from Chick Optic Lobe: Biochemical, Immunological, and Pharmacological Characterization

Channel Ligands Used to Probe Role of Voltage-Sensitive Calcium-Ion Channels in Neuropeptide Release

Modulation of Acetylcholine Release by Calcium Channel Antagonists

Capsaicin: A Probe for Studying Specific Neuronal Populations in Brain and Retina

Charybdotoxin in Study of Voltage-Dependent Potassium Channels

Ciguatoxin: A Tool for Research on Sodium-Dependent Mechanisms

Purification and Radiolabeling of Clostridium botulinium Type F Neurotoxin

Retrograde Tracing with Cholera Toxin B*b1Gold or with Immunocytochemically Detected Cholera Toxin B in Central Nervous System

*gu-Conotoxin: Calcium Currents and Neurosecretion

*gu-Conotoxin GVIA and Its Receptors

Dendrotoxin Acceptor Sites: Identification and Labeling of Brain Potassium Channels

Endothelins and Sarafotoxin in Neural Tissues

Fasciculin: Neuropharmacology of Potent Anticholinesterase Polypeptide

Geographutoxins

Presynaptic Activity of *ga-Latrotoxin: Purification and Properties

Natural Toxins in Study of Degeneration and Regeneration of Skeletal Muscle

Neosurugatoxin: A Probe for Neuronal Nicotinic Receptors in Adrenal Medulla, Brain, and Ganglia

Neurotoxins as Tools in Characterization of *gg-Aminobutyric Acid-Activated Chloride Channels

Palytoxin: Characterization of Mode of Action in Excitable Cells

Palytoxin: Mechanism of Acidifying Action in Excitable Cells

Pertussis Toxin in Analysis of Receptor Mechanisms

Resiniferatoxin

Scorpion Toxins Affecting Insects

Potassium Channel-Blocking Toxins from Snake Venoms and Neuromuscular Transmission

Each chapter includes references

Index

Table of Contents provided by Publisher. All Rights Reserved.