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Microbiology

by
Edition:
6th
ISBN13:

9780072951752

ISBN10:
0072951753
Format:
Hardcover
Pub. Date:
3/2/2004
Publisher(s):
McGraw Hill (Manual)
List Price: $194.20

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This is the 6th edition with a publication date of 3/2/2004.
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Summary

Prescott, Harley and Klein's 6th edition provides a balanced, comprehensive introduction to all major areas of microbiology. Because of this balance, Microbiology, 6/e is appropriate for students preparing for careers in medicine, dentistry, nursing, and allied health, as well as research, teaching, and industry. Biology and chemistry are prerequisites.

Table of Contents

Preface x
Visual Preview xv
About the Authors xxii
Part I Introduction to Microbiology
The History and Scope of Microbiology
1(16)
The Discovery of Microorganisms
2(1)
The Conflict over Spontaneous Generation
2(5)
The Role of Microorganisms in Disease
7(4)
Techniques & Applications 1.1: The Scientific Method
9(1)
Disease 1.2: Molecular Koch's Postulates
10(1)
Industrial Microbiology and Microbial Ecology
11(1)
Members of the Microbial World
11(1)
The Scope and Relevance of Microbiology
12(2)
The Future of Microbiology
14(3)
The Study of Microbial Structure: Microscopy and Specimen Preparation
17(22)
Lenses and the Bending of Light
18(1)
The Light Microscope
18(8)
Preparation and Staining of Specimens
26(4)
Electron Microscopy
30(5)
Newer Techniques in Microscopy
35(4)
Procaryotic Cell Structure and Function
39(34)
An Overview of Procaryotic Cell Structure
40(4)
Microbial Diversity & Ecology 3.1: Monstrous Microbes
43(1)
Procaryotic Cell Membranes
44(3)
The Cytoplasmic Matrix
47(3)
The Nucleoid
50(2)
Microbial Diversity & Ecology 3.2: Living Magnets
51(1)
The Procaryotic Cell Wall
52(7)
Protein Secretion in Procaryotes
59(2)
Components External to the Cell Wall
61(6)
Chemotaxis
67(1)
The Bacterial Endospore
68(5)
Eucaryotic Cell Structure and Function
73(20)
An Overview of Eucaryotic Cell Structure
75(1)
The Cytoplasmic Matrix, Microfilaments, Intermediate Filaments, and Microtubules
76(2)
The Endoplasmic Reticulum
78(1)
The Golgi Apparatus
78(1)
Lysosomes and Endocytosis
79(3)
Eucaryotic Ribosomes
82(1)
Mitochondria
82(2)
Chloroplasts
84(1)
Microbial Diversity & Ecology 4.1: The Origin of the Eucaryotic Cell
84(1)
The Nucleus and Cell Division
85(3)
External Cell Coverings
88(1)
Cilia and Flagella
88(2)
Comparison of Procaryotic and Eucaryotic Cells
90(3)
Part II Microbial Nutrition, Growth, and Control
Microbial Nutrition
93(16)
The Common Nutrient Requirements
94(1)
Requirements for Carbon, Hydrogen, and Oxygen
94(1)
Nutritional Types of Microorganisms
95(1)
Requirements for Nitrogen, Phosphorus, and Sulfur
96(1)
Growth Factors
96(2)
Uptake of Nutrients by the Cell
98(4)
Culture Media
102(2)
Historical Highlights 5.1: The Discovery of Agar as a Solidifying Agent and the Isolation of Pure Cultures
103(1)
Isolation of Pure Cultures
104(5)
Techniques & Applications 5.2: The Enrichment and Isolation of Pure Cultures
105(4)
Microbial Growth
109(24)
The Growth Curve
110(4)
Measurement of Microbial Growth
114(3)
The Continuous Culture of Microorganisms
117(1)
The Influence of Environmental Factors on Growth
118(10)
Microbial Diversity & Ecology 6.1: Life above 100°C
124(4)
Microbial Growth in Natural Environments
128(5)
Control of Microorganisms by Physical and Chemical Agents
133(16)
Definition of Frequently Used Terms
134(1)
Techniques & Applications 7.1: Safety in the Microbiology Laboratory
134(1)
The Pattern of Microbial Death
135(1)
Conditions Influencing the Effectiveness of Antimicrobial Agent Activity
136(1)
The Use of Physical Methods in Control
136(6)
Techniques & Applications 7.2: Universal Precautions for Microbiology Laboratories
142(1)
The Use of Chemical Agents in Control
142(4)
Evaluation of Antimicrobial Agent Effectiveness
146(3)
Part III Microbial Metabolism
Metabolism: Energy, Enzymes, and Regulation
149(18)
Energy and Work
150(1)
The Laws of Thermodynamics
151(1)
Free Energy and Reactions
152(1)
The Role of ATP in Metabolism
153(1)
Oxidation-Reduction Reactions and Electron Carriers
153(3)
Enzymes
156(4)
The Nature and Significance of Metabolic Regulation
160(1)
Metabolic Channeling
160(1)
Control of Enzyme Activity
161(6)
Metabolism: Energy Release and Conservation
167(32)
An Overview of Metabolism
168(3)
The Breakdown of Glucose to Pyruvate
171(3)
Fermentations
174(4)
Historical Highlights 9.1: Microbiology and World War I
177(1)
The Tricarboxylic Acid Cycle
178(1)
Electron Transport and Oxidative Phosphorylation
179(6)
Anaerobic Respiration
185(1)
Catabolism of Carbohydrates and Intracellular Reserve Polymers
186(1)
Lipid Catabolism
187(1)
Protein and Amino Acid Catabolism
188(1)
Oxidation of Inorganic Molecules
188(2)
Microbial Diversity & Ecology 9.2: Acid Mine Drainage
190(1)
Photosynthesis
190(9)
Metabolism: The Use of Energy in Biosynthesis
199(22)
Principles Governing Biosynthesis
200(2)
Techniques & Applications 10.1: The Identification of Anabolic Pathways
202(1)
The Photosynthetic Fixation of CO2
202(1)
Synthesis of Sugars and Polysaccharides
203(2)
The Assimilation of Inorganic Phosphorus, Sulfur, and Nitrogen
205(4)
The Synthesis of Amino Acids
209(1)
Anaplerotic Reactions
209(2)
The Synthesis of Purines, Pyrimidines, and Nucleotides
211(3)
Lipid Synthesis
214(2)
Peptidoglycan Synthesis
216(1)
Patterns of Cell Wall Formation
217(4)
Part IV Microbial Molecular Biology and Genetics
Genes: Structure, Replication, and Mutation
221(32)
DNA as Genetic Material
222(2)
Nucleic Acid Structure
224(5)
Historical Highlights 11.1: The Elucidation of DNA Structure
227(2)
DNA Replication
229(5)
The Genetic Code
234(1)
Gene Structure
235(4)
Mutations and Their Chemical Basis
239(6)
Detection and Isolation of Mutants
245(3)
DNA Repair
248(5)
Genes: Expression and Regulation
253(32)
DNA Transcription or RNA Synthesis
254(6)
Microbial Tidbits 12.1: Catalytic RNA (Ribozymes)
259(1)
Protein Synthesis
260(10)
Regulation of mRNA Synthesis
270(4)
Historical Highlights 12.2: The Discovery of Gene Regulation
272(2)
Attenuation
274(2)
Global Regulatory Systems
276(2)
Small RNAs and Regulation
278(1)
Two-Component Phosphorelay Systems
279(1)
Control of the Cell Cycle
280(5)
Microbial Recombination and Plasmids
285(26)
Bacterial Recombination: General Principles
286(2)
Bacterial Plasmids
288(3)
Disease 13.1: Virulence Plasmids
291(1)
Transposable Elements
291(5)
Bacterial Conjugation
296(3)
DNA Transformation
299(2)
Transduction
301(5)
Mapping the Genome
306(1)
Recombination and Genome Mapping in Viruses
307(4)
Part V DNA Technology and Genomics
Recombinant DNA Technology
311(24)
Historical Perspectives
312(3)
Synthetic DNA
315(1)
The Polymerase Chain Reaction
316(3)
Preparation of Recombinant DNA
319(3)
Cloning Vectors
322(4)
Inserting Genes into Eucaryotic Cells
326(1)
Expression of Foreign Genes in Bacteria
327(2)
Techniques & Applications 14.1: Gene Expression and Kittyboo Colors
329(1)
Applications of Genetic Engineering
329(3)
Techniques & Applications 14.2: Plant Tumors and Nature's Genetic Engineer
331(1)
Social Impact of Recombinant DNA Technology
332(3)
Microbial Genomics
335(16)
Introduction
336(1)
Determining DNA Sequences
336(1)
Whole-Genome Shotgun Sequencing
336(3)
Bioinformatics
339(1)
General Characteristics of Microbial Genomes
339(6)
Functional Genomics
345(4)
The Future of Genomics
349(2)
Part VI The Viruses
The Viruses: Introduction and General Characteristics
351(20)
Early Development of Virology
352(1)
Historical Highlights 16.1: Disease and the Early Colonization of America
353(1)
General Properties of Viruses
353(1)
The Cultivation of Viruses
353(2)
Virus Purification and Assays
355(3)
The Structure of Viruses
358(9)
Principles of Virus Taxonomy
367(4)
Microbial Tidbits 16.2: The Origin of Viruses
369(2)
The Viruses: Bacteriophages
371(16)
Classification of Bacteriophages
372(1)
Microbial Diversity & Ecology 17.1: An Ocean of Viruses
372(1)
Reproduction of Double-Stranded DNA Phages: The Lytic Cycle
373(5)
Reproduction of Single-Stranded DNA Phages
378(1)
Reproduction of RNA Phages
379(1)
Temperate Bacteriophages and Lysogeny
380(7)
The Viruses: Viruses of Eucaryotes
387(22)
Classification of Animal Viruses
388(1)
Reproduction of Animal Viruses
388(10)
Techniques & Applications 18.1: Constructing a Virus
396(2)
Cytocidal Infections and Cell Damage
398(1)
Persistent, Latent, and Slow Virus Infections
399(1)
Viruses and Cancer
400(1)
Plant Viruses
401(3)
Viruses of Fungi, Algae, and Protozoa
404(1)
Insect Viruses
404(1)
Viroids and Prions
405(4)
Part VII The Diversity of the Microbial World
Microbial Taxonomy and Phylogeny
409(28)
General Introduction and Overview
410(1)
Microbial Evolution and Diversity
410(2)
Taxonomic Ranks
412(2)
Classification Systems
414(2)
Major Characteristics Used in Taxonomy
416(4)
Assessing Microbial Phylogeny
420(3)
The Major Divisions of Life
423(5)
Microbial Diversity & Ecology 18.1: ``Official'' Nomenclature Lists---A Letter from Bergey's
428(1)
Bergey's Manual of Systematic Bacteriology
428(3)
A Survey of Procaryotic Phylogeny and Diversity
431(6)
The Archaea
437(16)
Introduction to the Archaea
438(4)
Phylum Crenarchaeota
442(2)
Phylum Euryarchaeota
444(9)
Microbial Diversity & Ecology 20.1: Methanotrophic Archaea
447(3)
Microbial Tidbits 20.2: Photosynthesis in Halobacterium salinarium
450(3)
Bacteria: The Deinococci and Nonproteobacteria Gram Negatives
453(20)
Aquificae and Thermotogae
454(1)
Deinococcus-Thermus
455(1)
Photosynthetic Bacteria
456(8)
Microbial Diversity & Ecology 21.1: The Mechanism of Gliding Motility
461(3)
Phylum Planctomycetes
464(1)
Phylum Chlamydiae
464(2)
Phylum Spirochaetes
466(3)
Phylum Bacteroidetes
469(4)
Bacteria: The Proteobacteria
473(30)
Class Alphaproteobacteria
474(8)
Class Betaproteobacteria
482(3)
Class Gammaproteobacteria
485(10)
Microbial Diversity & Ecology 22.1: Bacterial Bioluminescence
492(3)
Class Deltaproteobacteria
495(5)
Class Epsilonproteobacteria
500(3)
Bacteria: The Low G + C Gram Positives
503(18)
General Introduction
504(1)
Class Mollicutes (the Mycoplasmas)
504(3)
Low G + C Gram-Positive Bacteria in Bergey's Manual
507(1)
Class Clostridia
508(3)
Microbial Tidbits 23.1: Spores in Space
509(2)
Class Bacilli
511(10)
Bacteria: The High G + C Gram Positives
521(16)
General Properties of the Actinomycetes
522(2)
High G + C Gram-Positive Bacteria in Bergey's Manual
524(2)
Suborder Actinomycineae
526(1)
Suborder Micrococcineae
527(1)
Suborder Corynebacterineae
528(1)
Suborder Micromonosporineae
529(2)
Suborder Propionibacterineae
531(1)
Suborder Streptomycineae
531(2)
Suborder Streptosporangineae
533(1)
Suborder Frankineae
533(1)
Order Bifidobacteriales
534(3)
The Fungi (Eumycota), Slime Molds, and Water Molds
537(16)
Distribution
538(1)
Importance
539(1)
Structure
539(2)
Nutrition and Metabolism
541(1)
Reproduction
542(1)
Characteristics of the Fungal Divisions
543(5)
Slime Molds and Water Molds
548(5)
The Algae
553(12)
Distribution of Algae
555(1)
Classification of Algae
555(1)
Ultrastructure of the Algal Cell
556(1)
Algal Nutrition
556(1)
Structure of the Algal Thallus (Vegetative Form)
556(1)
Algal Reproduction
556(1)
Characteristics of the Algal Divisions
557(8)
Techniques & Applications 26.1: Practical Importance of Diatoms
560(2)
Disease 26.2: Toxic Algal Blooms
562(3)
The Protozoa
565(12)
Distribution
566(1)
Importance
566(1)
Morphology
566(1)
Nutrition
567(1)
Encystment and Excystment
568(1)
Locomotory Organelles
568(1)
Reproduction
568(1)
Classification
568(1)
Representative Types
569(8)
Microbial Diversity & Ecology 27.1: The Importance of Foraminiferans
572(5)
Part VIII Ecology and Symbiosis
Microorganism Interactions and Microbial Ecology
577(38)
Foundations of Microbial Ecology
578(1)
Microbial Interactions
578(15)
Microbial Diversity & Ecology 28.1: Microbial Ecology Versus Environmental Microbiology
579(5)
Microbial Diversity & Ecology 28.2: Coevolution of Animals and Their Gut Microbial Communities
584(9)
Nutrient Cycling Interactions
593(9)
The Physical Environment
602(5)
Techniques & Applications 28.3: Thermophilic Microorganisms and Modern Biotechnology
607(1)
Microbial Ecology and Its Methods: An Overview
607(8)
Microorganisms in Aquatic Environments
615(30)
Aquatic Environments and Microorganisms
616(5)
Disease 29.1: New Agents in Medicine--The Sea as the New Frontier
617(4)
The Microbial Community
621(3)
Marine Environments
624(4)
Freshwater Environments
628(2)
Waters and Disease Transmission
630(6)
Techniques & Applications 29.2: Waterborne Diseases, Water Supplies, and Slow Sand Filtration
632(4)
Wastewater Treatment
636(6)
Disease 29.3: Sewage Sludge: Long-Term Concerns with Land and Water Disposal
641(1)
Groundwater Quality and Home Treatment Systems
642(3)
Microorganisms in Terrestrial Environments
645(28)
Soils as an Environment for Microorganisms
646(1)
Microorganisms in the Soil Environment
647(1)
Microorganisms and the Formation of Different Soils
648(3)
Soil Microorganism Associations with Vascular Plants
651(11)
Microbial Diversity & Ecology 30.1: Mycorrhizae and the Evolution of Vascular Plants
656(6)
Soils, Plants, and Nutrients
662(2)
Microbial Tidbits 30.2: An Unintended Global-Scale Nitrogen Experiment
664(1)
Soils, Plants, and the Atmosphere
664(2)
Techniques & Applications 30.3: Keeping Inside Air Fresh with Soil Microorganisms
665(1)
Microbial Diversity & Ecology 30.4: Soils, Termites, Intestinal Microbes, and Atmospheric Methane
666(1)
Microorganisms and Plant Decomposition
666(1)
The Subsurface Biosphere
667(2)
Soil Microorganisms and Human Health
669(1)
Understanding Microbial Diversity in the Soil
670(3)
Part IX Nonspecific (Innate) Resistance and the Immune Response
Normal Microbiota and Nonspecific (Innate) Host Resistance
673(32)
Gnotobiotic Animals
674(1)
Normal Microbiota of the Human Body
675(5)
Techniques & Applications 31.1: Probiotics for Humans and Animals
679(1)
Overview of Host Resistance
680(1)
Cells, Tissues, and Organs of the Immune System
681(4)
Physical and Chemical Barriers in Nonspecific (Innate) Resistance
685(3)
Inflammation
688(3)
The Complement System
691(2)
Phagocytosis
693(4)
Cytokines
697(3)
Natural Killer Cells
700(5)
Specific (Adaptive) Immunity
705(34)
Overview of Specific (Adaptive) Immunity
706(2)
Antigens
708(2)
Antibodies
710(11)
Techniques & Applications 32.1: Immunotoxins
721(1)
T-Cell Biology
721(7)
Techniques & Applications 32.2: Donor Selection for Tissue or Organ Transplants
724(4)
B-Cell Biology
728(3)
Action of Antibodies
731(3)
The Classical Complement Pathway
734(1)
Acquired Immune Tolerance
734(1)
Summary: The Role of Antibodies and Lymphocytes in Resistance
734(5)
Medical Immunology
739(22)
Vaccines and Immunizations
740(4)
Historical Highlights 33.1: The First Immunizations
740(4)
Immune Disorders
744(7)
Antigen-Antibody Interactions In Vitro
751(10)
Techniques & Applications 33.2: History and Importance of Serotyping
757(4)
Part X Microbial Diseases and Their Control
Pathogenicity of Microorganisms
761(18)
Host-Parasite Relationships
762(2)
Pathogenesis of Viral Diseases
764(1)
Pathogenesis of Bacterial Diseases
765(10)
Techniques & Applications 34.1: Detection and Removal of Endotoxins
774(1)
Microbial Mechanisms for Escaping Host Defenses
775(4)
Antimicrobial Chemotherapy
779(20)
The Development of Chemotherapy
780(1)
Techniques & Applications 35.1: The Use of Antibiotics in Microbiological Research
781(1)
General Characteristics of Antimicrobial Drugs
781(2)
Determining the Level of Antimicrobial Activity
783(1)
Mechanisms of Action of Antimicrobial Agents
784(2)
Factors Influencing the Effectiveness of Antimicrobial Drugs
786(1)
Antibacterial Drugs
787(5)
Drug Resistance
792(3)
Disease 35.2: Antibiotic Misuse and Drug Resistance
793(2)
Antifungal Drugs
795(1)
Antiviral Drugs
796(3)
Clinical Microbiology
799(22)
Specimens
800(4)
Techniques & Applications 36.1: Universal Precautions for Health-Care Professionals
802(2)
Identification of Microorganisms from Specimens
804(13)
Techniques & Applications 36.2: Monoclonal Antibodies in Clinical Microbiology
813(4)
Susceptibility Testing
817(1)
Computers in Clinical Microbiology
817(4)
The Epidemiology of Infectious Disease
821(24)
Epidemiological Terminology
822(1)
Historical Highlights 37.1: John Snow---The First Epidemiologist
822(1)
Measuring Frequency: The Epidemiologist's Tools
823(1)
Infectious Disease Epidemiology
823(1)
Recognition of an Infectious Disease in a Population
824(1)
Recognition of an Epidemic
824(3)
Historical Highlights 37.2: ``Typhoid Mary''
825(2)
The Infectious Disease Cycle: Story of a Disease
827(5)
Historical Highlights 37.3: The First Indications of Person-to-Person Spread of an Infectious Disease
831(1)
Virulence and the Mode of Transmission
832(1)
Emerging and Reemerging Infectious Diseases and Pathogens
832(5)
Disease 37.4: The SARS Epidemic of 2003
835(2)
Control of Epidemics
837(1)
The Emerging Threat of Bioterrorism
837(2)
Historical Highlights 37.5: 1346---The First Recorded Biological Warfare Attack
838(1)
Global Travel and Health Considerations
839(1)
Nosocomial Infections
840(5)
Human Diseases Caused by Viruses
845(30)
Airborne Diseases
846(6)
Disease 38.1: Reye's and Guillain-Barre Syndromes
849(3)
Arthropod-Borne Diseases
852(3)
Disease 38.2: Viral Hemorrhagic Fevers---A Microbial History Lesson
853(2)
Direct Contact Diseases
855(13)
Food-Borne and Waterborne Diseases
868(2)
Historical Highlights 38.3: A Brief History of Polio
870(1)
Slow Virus and Prion Diseases
870(1)
Other Diseases
871(4)
Human Diseases Caused by Bacteria
875(42)
Airborne Diseases
876(9)
Arthropod-Borne Diseases
885(4)
Historical Highlights 39.1: The Hazards of Microbiological Research
885(4)
Direct Contact Diseases
889(13)
Disease 39.2: Biofilms
897(3)
Disease 39.3: Resistant Staphylococci
900(2)
Disease 39.4: A Brief History of Syphilis
902(6)
Food-Borne and Waterborne Diseases
905(3)
Techniques & Applications 39.5: Clostridial Toxins as Therapeutic Agents---Benefits of Nature's Most Toxic Proteins
908(3)
Sepsis and Septic Shock
911(1)
Dental Infections
911(6)
Human Diseases Caused by Fungi and Protozoa
917(20)
Fungal Diseases
918(8)
Disease 40.1: The Emergence of Candidiasis
925(1)
Protozoan Diseases
926(11)
Disease 40.2: A Brief History of Malaria
930(7)
Part XI Food and Industrial Microbiology
Microbiology of Food
937(26)
Microorganism Growth in Foods
938(2)
Microbial Growth and Food Spoilage
940(3)
Controlling Food Spoilage
943(3)
Historical Highlights 41.1: An Army Travels on Its Stomach
944(2)
Food-Borne Diseases
946(3)
Historical Highlights 41.2: Typhoid Fever and Canned Meat
947(2)
Detection of Food-Borne Pathogens
949(2)
Microbiology of Fermented Foods
951(7)
Techniques & Applications 41.3: Starter Cultures, Bacteriophage Infections, and Plasmids
951(7)
Microorganisms as Foods and Food Amendments
958(5)
Industrial Microbiology and Biotechnology
963
Choosing Microorganisms for Industrial Microbiology and Biotechnology
964(6)
Techniques & Applications 42.1: The Potential of Thermophilic Archaea in Biotechnology
965(5)
Microorganism Growth in Controlled Environments
970(4)
Major Products of Industrial Microbiology
974(5)
Microbial Growth in Complex Natural Environments
979(7)
Microbial Diversity & Ecology 42.2: Methanogens---A New Role for a Unique Microbial Group
981(4)
Microbial Diversity & Ecology 42.3: A Fungus with a Voracious Appetite
985(1)
Biotechnological Applications
986(4)
Techniques & Applications 42.4: Streptavidin-Biotin Binding and Biotechnology
987(3)
Impacts of Microbial Biotechnology
990
Appendices
Appendix I A Review of the Chemistry of Biological Molecules
A-1(1)
Appendix II Common Metabolic Pathways
A-11(1)
Appendix III Classification of Procaryotes According to the Second Edition of Bergey's Manual of Systematic Bacteriology
A-19(1)
Appendix IV Classification of Viruses
A-28(1)
Appendix V Recommended Readings
A-38(1)
Glossary G-1(1)
Credits C-1(1)
Index I-1


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