Microbiology Experiments : A Health Science Perspective

by Kleyn, John; Bicknell, Mary

• ISBN13: 9780072476248
• ISBN10: 0072476249
• Edition: 4th
• Format: Paperback
• Copyright: 2003-02-14
• Publisher: McGraw-Hill Science/Engineering/Math

Summary

All experiments are correlated to Nester's Microbiology: A Human Perspective, 4/e, but can be used with any non-majors/allied health microbiology textbook.

Table of Contents

Preface	vii
Laboratory Safety	viii
Laboratory Safety Agreement	ix
PART ONE Basic Microbiology	1	(146)
Introduction to Microbiology	1	(2)
Ubiquity of Microorganisms	3	(6)
Bright-field Light Microscopy, Including History and Working Principles	9	(12)
Microscopic (Bright-field and Dark-field) Determination of Cell Motility, Form, and Viability Using Wet Mount and Hanging Drop Preparations	21	(10)
Introduction to Staining of Microorganisms	29	(2)
Simple Stains: Positive and Negative Stains	31	(6)
Multiple and Differential Stains	37	(14)
Introduction to Microbial Growth	49	(2)
Pure Culture and Aseptic Technique	51	(6)
Defined, Undefined, Selective, and Differential Media	57	(4)
Quantification of Microorganisms	61	(10)
Introduction to the Environment and Microbial Growth	69	(2)
Aerobic and Anaerobic Growth	71	(4)
The Effect of Incubation Temperature on Generation Time	75	(10)
Introduction to Control of Microbial Growth	83	(2)
Moist and Dry Heat Sterilization: Thermal Death Point and Thermal Death Time	85	(10)
Control of Microbial Growth with Ultraviolet Light	95	(6)
Osmotic Pressure, and Its Effect on the Rate and Amount of Microbial Growth	101	(8)
Antiseptics and Antibiotics	109	(12)
Introduction to Microbial Genetics	119	(2)
Selection of Bacterial Mutants Resistant to Antibiotics	121	(6)
Transformation: A Form of Genetic Recombination	127	(6)
Bacterial Conjugation	133	(8)
Gene Regulation: Induction and Catabolite Repression	141	(6)
PART TWO The Other Microbial World	147	(44)
Introduction to the Other Microbial World	147	(4)
Microscopic Identification of Fungi	151	(18)
Parasitology: Protozoa and Helminths	169	(14)
Prokaryotic Viruses: Bacteriophage Isolation and Titering	183	(8)
PART THREE Microbes and Humans	191	(28)
Introduction to Medical Microbiology	191	(2)
Normal Skin Flora	193	(6)
Respiratory Microorganisms	199	(6)
Identification of Enteric Gram-Negative Rods	205	(6)
Clinical Unknown Identification	211	(8)
PART FOUR Immunology	219	(52)
Introduction to Some Immunological Principles and Techniques	219	(2)
Differential White Blood Cell Stains	221	(6)
Lysozyme, an Enzymatic Form of Natural Resistance	227	(6)
Traditional Agglutination Reactions Employing Microbial and Nonmicrobial Antigens	233	(12)
Lancefield Grouping of Pathogenic Streptococci with a Latex Slide Agglutination Test	245	(8)
Use of an Enzyme-Linked Immunosorbent Assay (ELISA) Test for Coccidioides immitis Identification	253	(8)
An Ouchterlony Double Immunodiffusion Test for Coccidioides immitis Identification	261	(10)
PART FIVE Public Health	271	(28)
Introduction to the Preention and Control of Communicable Diseases	271	(2)
Epidemiology: A Staphylococcus Carrier Study	273	(12)
Bacteriological Examination of Water: Multiple-Tube Fermentation and Membrane Filter Techniques	285	(14)
PART SIX Applications of Biotechnology	299	(14)
Introduction to Biotechnology	299	(2)
Identifying DNA with Restriction Enzymes	301	(6)
Identification of Bacteria Using the Ribosomal Data Project	307	(6)
PART SEVEN Projects	313	(24)
Introduction to the Individual Projects	313	(2)
Hydrocarbon-Degrading Bacteria, Cleaning Up After Oil Spills	315	(2)
Luminescent Bacteria: Bacteria That Produce Light	317	(2)
Methylotrophs, Organisms That Grow on One-Carbon Compounds	319	(2)
Deinococcus, Bacteria with Out-Of-this-World Capabilities	321	(16)
APPENDICES
1. Living Microorganisms (Bacteria, Fungi, Protozoa, and Helminths) Chosen for Study in this Manual	323	(2)
2. Dilution Practice Problems	325	(2)
3. Metric System, Use of with Conversions to the English System of Measurement	327	(2)
4. pH Adjustment of Liquid and Agar Growth Media	329	(2)
5. Use of the Ocular Micrometer for Measurement of Relative and Absolute Cell Size	331	(2)
6. Use of the Hemocytometer for Determining Total Cell Number in a Liquid Suspension	333	(2)
7. Preparation of Covered Slide Cultures for Study of Intact Structure of a Mold Thallus	335	(2)
Index	337