Microbiology Experiments: A Health Science Perspective

by Kleyn Et Al

• ISBN13: 9780077315542
• ISBN10: 0077315545
• Edition: 7th
• Format: Spiral Bound
• Copyright: 2011-10-25
• Publisher: McGraw-Hill Education

Summary

All experiments are correlated to Nester's Microbiology: A Human Perspective, 7e, but can be used with any non-majors/allied health microbiology textbook.

Table of Contents

PART ONE

Basic Microbiology 1

Introduction to Microbiology 1

1. Ubiquity of Microorganisms 3

2. Pure Culture and Aseptic Technique 9

3. Introduction to the Compound Light Microscope 17

4. The Oil Immersion Lens 23Introduction to Staining of Microorganisms 29

5. Simple Stains: Positive and Negative Stains 31

6. Differential and Other Special Stains 37Introduction to Microbial Growth 45

7. Chemically Defined, Complex, Selective, and Differential Media 47

8. Quantification of Microorganisms 51Introduction to the Environment and Microbial Growth 59

9. Aerobic and Anaerobic Growth 61

10. The Effect of Incubation Temperature on Generation Time 65

Introduction to Control of Microbial Growth 73

11. Moist and Dry Heat Sterilization: Thermal Death Point and Thermal Death Time 75

12. Control of Microbial Growth with Ultraviolet Light 87

13. Changes in Osmotic Pressure Due to Salt and Sugar and Their Effects on Microbial Growth 95

14. Antibiotics 103

15. Antiseptics and Disinfectants 109

Introduction to Microbial Genetics 115

16. Selection of Bacterial Mutants Resistant to Antibiotics 117

17. Transformation: A Form of Genetic Recombination 123

18. Gene Regulation: Induction and Catabolite Repression 129

PART TWO

The Other Microbial World 135

Introduction to the Other Microbial World 135

l9. Microscopic Identification of Fungi 139

20. Parasitology: Protozoa and Helminths 155

21. Titering Prokaryotic Viruses 171

PART THREE

Microbes and Humans 179

Introduction to Medical Microbiology 179

22. Normal Skin Biota 181

23. Streptococci and Respiratory Microorganisms 189

24. Identification of Enteric Gram-Negative Rods 197

25. Clinical Unknown Identification 207

PART FOUR

Immunology 215

Introduction to Some Immunological Principles and Techniques 215

26. Differential White Blood Cell Stains 217

27. Lysozyme, an Enzyme Contributing to Innate Immunity 223

28. Enzyme-Linked Immunoabsorbent Assay (ELISA) 229

PART FIVE

Public Health 235

Introduction to the Prevention and Control of Communicable Diseases 235

29. Bacteriological Examination of Water: Multiple-Tube Fermentation and Membrane Filter Techniques 237

30. Epidemiology 251

PART SIX

Applications of Biotechnology 255

Introduction to Biotechnology 255

31. Identifying DNA with Restriction Enzymes and the Polymerase Chain Reaction (PCR) 257

32. Electrophoresis 265

33. Identification of Bacteria Using the Ribosomal Database Project 271

34. Microarray 277

PART SEVEN

Individual Projects 283

Introduction to the Individual Projects 283

35. Hydrocarbon-Degrading Bacteria: Cleaning Up after Oil Spills 285

36. Luminescent Bacteria: Bacteria That Produce Light 289

APPENDICES

1. Culture Media and Reagent Formulas 291

2. Living Microorganisms (Bacteria, Fungi, Protozoa, and Helminths) Chosen for Study in This Manual 297

3. Dilution Practice Problems 299

4. Alternative Procedures 301

5. Use of the Ocular Micrometer for Measurement of Relative and Absolute Cell Size 303

6. History and Working Principles of Light

Microscopy 305

Index 309