Molecular Approaches to Soil, Rhizosphere and Plant Microorganism Analysis

by J. E. Cooper; J. R. Rao

• ISBN13: 9781845930622
• ISBN10: 1845930622
• Format: Hardcover
• Copyright: 2007-06-14
• Publisher: Cab Intl

Summary

Using contributions by international researchers, this book analyses the molecular methods used for studying microorganisms that occur in the soil or that are associated with plants, at the community, population, taxonomic and functional group levels. By providing a critical and comparative basis upon which an informed choice of technique can be made, this book empowers a prospective researcher to select a method that is most appropriate for their requirements.

Table of Contents

Contributors	p. vii
Preface	p. ix
Genomic Analyses of Microbial Processes in Biogeochemical Cycles	p. 1
Applications of Nucleic Acid Microarrays in Soil Microbial Ecology	p. 18
Metagenomics for the Study of Soil Microbial Communities	p. 42
In Vivo Expression Technology (IVET) for Studying Niche-specific Gene Expression by Plant- and Soil-colonizing Bacteria	p. 55
Analysing Microbial Community Structure by Means of Terminal Restriction Fragment Length Polymorphism (T-RFLP)	p. 84
Characterization of Phylloplane and Rhizosphere Microbial Populations Using PCR and Denaturing Gradient Gel Electrophoresis (DGGE)	p. 99
Molecular Tools for Studying Plant Growth-promoting Rhizobacteria (PGPR)	p. 116
Detection of Autotrophic Sulphur- and Iron-oxidizing Bacteria Using Labelled Fatty Acid Methyl Esters (FAMEs)	p. 132
Molecular Analyses of Soil Denitrifying Bacteria	p. 146
Ecology of Streptomyces in Soil and Rhizosphere	p. 166
Molecular Ecology of Ectomycorrhizal Fungal Communities: New Frontiers	p. 183
Molecular Ecology of Arbuscular Mycorrhizal Fungi: a Review of PCR-based Techniques	p. 198
Transcriptomics for Determining Gene Expression in Symbiotic Root-Fungus Interactions	p. 213
Differentiation of Nitrogen-fixing Legume Root Nodule Bacteria (Rhizobia)	p. 236
Molecular Markers for Studying the Ecology of Rhizobia	p. 259
Molecular Characterization of Bacterial Plant Pathogens	p. 272
Index	p. 293
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