Molecular Embryology

by Sharpe, Paul T.; Mason, Ivor

• ISBN13: 9780896033870
• ISBN10: 0896033872
• Format: Hardcover
• Copyright: 1999-09-01
• Publisher: Humana Pr Inc

Summary

A comprehensive guide to cutting-edge methods used today across the dramatically growing field of molecular embryology. These powerful techniques take advantage of the most commonly used vertebrate experimental models: murine embryos for their genetics, chick embryos for in vivo manipulation, zebrafish for mutagenesis, amphibian embryos, and nonvertebrate chordates. The book emphasizes the most important methods of experimental molecular biology and the particular advantages of each different species. Detailed, easy-to-follow instructions, together with relevant background information and helpful tips, ensure easy reproducibility.

Table of Contents

Preface	v	(2)
List of Color Plates	vii	(6)
Contributors	xiii
PART I. THE MOUSE EMBRYO	1	(212)
1 The Mouse as a Developmental Model	3	(4)
Paul T. Sharpe
2 Culture of Postimplantation Mouse Embryos	7	(16)
Paul Martin
David L. Cockroft
3 Organ Culture in the Analysis of Tissue Interactions	23	(10)
Irma Thesleff
Carin Sahlberg
4 Treatment of Mice with Retinoids In Vivo and In Vitro: Skeletal Staining	33	(8)
Gillian M. Morriss-Kay
5 Cell Grafting and Labeling in Postimplantation Mouse Embryos	41	(20)
Gabriel A. Quinlan
Paul A. Trainor
Patrick P. L. Tam
6 Production of Transgenic Rodents by the Microinjection of Cloned DNA into Fertilized One-Celled Eggs	61	(40)
San Ling Si-Hoe
David Murphy
7 Cre Recombinase Mediated Alterations of the Mouse Genome Using Embryonic Stem Cells	101	(22)
Anna-Katerina Hadjantonakis
Melinda Pirity
Andras Nagy
8 Gene Trapping in Mouse Embryonic Stem Cells	123	(16)
Jane Brennan
William C. Skarnes
9 Production and Growth of Conditionally Immortal Cell Lines from the H-2K(b)tsA58 Transgenic Mouse	139	(20)
Mark Noble
10 Reporter Genes for the Study of Transcriptional Regulation in Transgenic Mouse Embryos	159	(24)
Jonathan D. Gilthorpe
Peter W. J. Rigby
11 Application of lacZ Transgenic Mice to Cell Lineage Studies	183	(18)
Paul A. Trainor
Sheila X. Zhou
Maala Parameswaran
Gabriel A. Quinlan
Monica Gordon
Karin Sturm
Patrick P. L. Tam
12 Mouse Primordial Germ Cells: Isolation and In Vitro Culture	201	(12)
Patricia A. Labosky
Brigid L. M. Hogan
PART II. CHICKEN EMBRYO	213	(116)
13 The Avian Embryo: An Overview	215	(6)
Ivor Mason
14 Chick Embryos: Incubation and Isolation	221	(4)
Ivor Mason
15 New Culture	225	(20)
Amata Hornbruch
16 Grafting Hensen's Node	245	(10)
Claudio D. Stern
17 Grafting of Somites	255	(10)
Claudio D. Stern
18 Notochord Grafts	265	(8)
Andrew Lumsden
Susanne Dietrich
19 Transplantation of Avian Neural Tissue	273	(8)
Sarah Guthrie
20 Grafting of Apical Ridge and Polarizing Region	281	(12)
Cheryl Tickle
21 Tissue Recombinations in Collagen Gels	293	(12)
Marysia Placzek
Kim Dale
22 Quail-Chick Chimeras	305	(14)
Marie-Aimee Teillet
Catherine Ziller
Nicole M. Le Douarin
23 Using Fluorescent Dyes for Fate Mapping, Lineage Analysis, and Axon Tracing in the Chick Embryo	319	(10)
Jonathan D. W. Clarke
PART III. AMPHIBIAN EMBRYO	329	(100)
24 An Overview of Xenopus Development	331	(10)
C. Michael Jones
James C. Smith
25 Mesoderm Induction Assays	341	(10)
C. Michael Jones
James C. Smith
26 Experimental Embryological Methods for Analysis of Neural Induction in the Amphibian	351	(42)
Ray Keller
Ann Poznanski
Tamira Elul
27 A Method for Generating Transgenic Frog Embryos	393	(22)
Enrique Amaya
Kristen L. Kroll
28 Axolotl/newt	415	(14)
Malcolm Maden
PART IV. ZEBRAFISH	429	(82)
29 The Zebrafish: An Overview of Its Early Development	431	(10)
Nigel Holder
Qiling Xu
30 Small-Scale Marker-Based Screening for Mutations in Zebrafish Development	441	(20)
Peter D. Currie
Thomas F. Schilling
Philip W. Ingham
31 Transgenic Zebrafish	461	(26)
Trevor Jowett
32 Microinjection of DNA, RNA, and Protein into the Fertilized Zebrafish Egg for Analysis of Gene Function	487	(4)
Nigel Holder
Qiling Xu
33 Retinoids in Nonmammalian Embryos	491	(20)
Malcolm Maden
PART V. NONVERTEBRATE CHORDATES	511	(6)
34 Protochordates	513	(4)
Peter W. H. Holland
Hiroshi Wada
PART VI. RETROVIRUSES	517	(36)
35 Gene Transfer to the Rodent Embryo by Retroviral Vectors	519	(20)
Grace K. Pavlath
Marla B. Luskin
36 Gene Transfer in Avian Embryos Using Replication-Competent Retroviruses	539	(14)
Cairine Logan
Philippa Francis-West
PART VII. MOLECULAR TECHNIQUES	553	(156)
37 Subtractive Hybridization and Construction of cDNA Libraries	555	(20)
Bruce Blumberg
Juan Carlos Izpisua Belmonte
38 Differential Display of Eukaryotic mRNA	575	(16)
Antonio Tugores
Juan Carlos Izpisua Belmonte
39 RT-PCR on Embryos Using Degenerate Oligonucleotide Primers	591	(10)
Anthony Graham
40 Single-Cell RT-PCR cDNA Subtraction	601	(10)
Damian L. Weaver
Cesar Nunez
Clare Brunet
Victoria Bostock
Gerard Brady
41 In Situ Hybridization of Radioactive Riboprobes to RNA in Tissue Sections	611	(12)
Radma Mahmood
Ivor Mason
42 In Situ Hybridization to RNA in Whole Embryos	623	(12)
Huma Shamim
Radma Mahmood
Ivor Mason
43 Wholemount In Situ Hybridization to Xenopus Embryos	635	(6)
C. Michael Jones
James C. Smith
44 Whole-Mount In Situ Hybridization to Amphioxus Embryos	641	(4)
Peter W. H. Holland
45 In Situ Hybridization to Sections (Nonradioactive)	645	(10)
Maria Rex
Paul J. Scotting
46 Immunohistochemistry Using Polyester Wax	655	(8)
Andrew Kent
47 Immunohistochemistry on Whole Embryos	663	(4)
Ivor Mason
48 Whole Embryo Assays for Programmed Cell Death	667	(6)
Anthony Graham
49 Gene Interference Using Antisense Oligodeoxynucleotides on Whole Chick Embryos: Optimal Ring and Roller-Bottle Culture Technique	673	(26)
Jonathan Cooke
Alison Isaac
50 Protein Techniques: Immunoprecipitation, In Vitro Kinase Assays, and Western Blotting	699	(10)
David I. Jackson
Clive Dickson
PART VIII. MICROSCOPY AND PHOTOGRAPHY	709	(26)
51 Microscopy and Photomicrography Techniques	711	(24)
Richard J. T. Wingate
Index	735