PCR Technology Principles and Applications for DNA Amplification

by Erlich, Henry A.

• ISBN13: 9780195098754
• ISBN10: 0195098757
• Format: Paperback
• Copyright: 1993-09-23
• Publisher: Oxford University Press

Summary

Polymerase chain reaction (PCR) technology is a revolutionary innovation which enables scientists to rapidly generate large amounts of genetic material from a slight trace which would otherwise be too small to analyze. With applications in both research and diagnostics, PCR is becoming a standard procedure in biotechnology and medical diagnostic laboratories. This book is an introduction and guide to the new technology, covering the basic methodologies and their applications in research and medicine, emphasizing practical aspects. Each chapter is written by pioneers in the field and most include detailed protocols and favorite PCR "recipes". Students and researchers in all areas of biotechnology and molecular biology will find this book the introduction to PCR they've been looking for.

Table of Contents

Basic Methodology

The Design and Optimization of the PCR

Taq DNA Polymerase

PCR Automatin

Simple and Rapid Preparation of Samples for PCR

Research Applications

Direct Sequencing of In Vitro Amplified DNA

Using PCR to Engineer DNA

Mutation Detection by PCR, GC-Clamps, and Denaturing Gradient Gel Electrophoresis

Detection of Gene Expression

PCR Amplification of Specific Sequences from cDNA Library

Inverse Polymerase Chain Reaction

Alu PCR: The Use of Repeat Sequence Primers for Amplification of Human DNA from Complex Sources

A New Approach to Constructing Genetic Maps: PCR Analysis of DNA Sequences in Individual Gametes

Evolutionary Analysis via PCR

Medical Applications

Use of PCR in the Diagnosis of Monogenic Disease

Diagnosis of New Mutation Diseases Using the Polymerase Chain Reaction

HLA Class II Gene Polymorphism: DNA Typing, Evolution, and Relationship to Disease Susceptibility

Applications of PCR to the Analysis of Biological Evidence

Detection of ras Oncogenes Using PCR

Application of PCR to the Detection of Human Infectious Diseases

Table of Contents provided by Publisher. All Rights Reserved.