Phytoplankton Pigments

by Roy, Suzanne; Llewellyn, Carole A.; Egeland, Einar Skarstad; Johnsen, Geir

• ISBN13: 9781107000667
• ISBN10: 1107000661
• Format: Hardcover
• Copyright: 2011-12-29
• Publisher: Cambridge Univ Pr

Summary

Pigments act as tracers to elucidate the fate of phytoplankton in the world's oceans and are often associated with important biogeochemical cycles related to carbon dynamics in the oceans. They are increasingly used in in situ and remote-sensing applications, detecting algal biomass and major taxa through changes in water colour. This book is a follow-up to the 1997 volume Phytoplankton Pigments in Oceanography (UNESCO Press). Since then, there have been many advances concerning phytoplankton pigments. This book includes recent discoveries on several new algal classes particularly for the picoplankton, and on new pigments. It also includes many advances in methodologies, including liquid chromatography-mass spectrometry (LC-MS) and developments and updates on the mathematical methods used to exploit pigment information and extract the composition of phytoplankton communities. The book is invaluable primarily as a reference for students, researchers and professionals in aquatic science, biogeochemistry and remote sensing.

Table of Contents

List of contributors

Preface

Acknowledgements

List of symbols

Chlorophylls and Carotenoids

Microalgal classes and their signature pigments

Recent advances in chlorophyll and bacteriochlorophyll biosynthesis

Carotenoid metabolism in phytoplankton

Methodology Guidance

New HPLC separation techniques

The importance of a quality assurance plan for method validation and minimizing uncertainties in the HPLC analysis of phytoplankton pigments

Appendix:a symbology and vocabulary for an HPLC lexicon

Quantitative interpretation of chemotaxonomic pigment data

Liquid chromatography-mass spectrometry for pigment analysis

Multivariate analysis of extracted pigments using spectrophotometric and spectrofluorometric methods

Appendix:a proven simultaneous equation assay for chlorophylls a and b using aqueous acetone and similar assays for recalcitrant algae

Water-Soluble 'Pigments'

Phycobiliproteins

UV-absorbing 'pigments': mycosporine-like amino acids

Selected Pigment Applications in Oceanography

Pigments and photoacclimation processes

Pigment-based measurements of phytoplankton rates

In vivo bio-optical properties of phytoplankton pigments

Optical monitoring of phytoplankton bloom pigment signatures

Appendix:harmful algae toxins and pigments

Future Perspectives

Perspectives on future directions

Aids for Practical Laboratory Work

Update on filtration, storage and extraction solvents

The pigment analyst's guide to HPLC hardware

Minimum identification criteria for identifying phytoplankton pigments

Phytoplankton cultures for standard pigments and their suppliers

Commercial suppliers of phytoplankton pigments

Phytoplankton pigments data sheets

Index

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